GoEditStem Services

GoEditStem platform cores offer a variety of services related to creating CRISPR knock-outs in cells.  

FinGEEC

(primary cells and cells from other species, if feasible) 

Consultation or +358 2941 25494 (9-16) 

BSCC

GBU

Services related to CRISPR animal models are provided by Comprehensive Model Organisms platform

HelVi-BVC

HelVi-AAV

(AAV) suitable for transduction of animals and cultured cells. 

FinGEEC

core facility provides comprehensive human pluripotent stem cell (hPSC) services at the University of Helsinki’s Meilahti campus. hPSC disease-specific models, coupled with advancements in genome editing technologies, enable valuable insights into pathophysiological mechanisms in disease-relevant systems. The is responsible for iPSC differentiation into different brain cell types. 

BSCC

NiPS

Genome Biology Unit hosts clone libraries and provides cloning services to a variety of vectors. GBU also works with pooled DNA libraries. 

Generation of transgene-expressing cells is done by . and can provide the constructs and virus preps, if applicable. 

Step 1.  Selecting a construct of interest from or use customer's own construct

Step 2. Recombinant virus preps is generated using the selected construct

Step 3. a) Customer provides mycoplasma-tested target cells to

• immortalized cell line
• iPSC cells from
• primary cells

Step 3. b) Kill-curve for antibiotic selection is generated 

Step 4. Transduction of the target cells

Step 5. Selection and expansion of the transduced cells

Step 6: Customer receives two frozen ampoules per each construct of Biosafety-level one (BSL1) ready cells 

NOTE
Only need the virus prep? Choose steps 1-2
Already have your virus? Choose steps 3-6

Generation of CRISPR-edited cells is done by or (iPSCs). and can provide the constructs and virus preps, if applicable. 

Step 1. Choose your construct with the guide from or order custom guide design and cloning (, ). 

Step 2. Recombinant virus preps is generated using the selected construct () (OPTIONAL) 

Step 3. Customer provides mycoplasma-tested target cells 

• immortalized cell line
• primary cells

Step 4. Delivery of the editing components into the target cells via transfection, electroporation, or transduction 

Step 6. Selection expansion of edited cells

NOTE
You can also provide your own construct or virus and start from step 3. 

can clone your cDNA of interest in an AAV-compatible vector using the Gateway system. can then use the vectors to produce rAAV particles. 

Step 1, choose one:
A) Select a cDNA clone from the Gateway-compatible  
B) Obtain a Gateway-compatible pENTR clone with your favourite cDNA from a commercial supplier.
C) Any cDNA template can be converted to Gateway system using PCR in .
(ideal cDNA length  1-2,5 kb)

Step 2: The cDNA is cloned to pAAV-Gateway using Gateway cloning.

Step 3: Helvi-AAV produces the virus prep of your choice. You can select the AAV serotype based on the desired cell-type specificity, and the scale of rAAV particle production (small scale 0,3 ml, large-scale 3 ml).

NOTE
You can also obtain an AAV vector containing your cDNA of interest from a commercial source and deliver it directly to HelVI-AAV. 

offers a fully equipped BSL2 room for working with lenti- and retroviruses in Biomedicum, 5th floor. 

offers a fully equipped BSL2 laboratory for growing human primary cells and iPSCs in Biomedicum, 2nd floor.

The IncuCyte S3 continuously acquires and analyzes images, supporting up to six microplates simultaneously. Users can schedule experiments with varying image acquisition frequencies and magnifications. The system also allows remote access with unlimited free licenses. Available IncuCyte S3 modules include Standard, Scratch Wound, Whole Well, Dilution Cloning, and Chemotaxis, along with Basic Analyzer and Neurotrack modules for analysis. 

provides basic training required for using the IncuCyte S3 system. User fee is time-based. 

For in vitro electrophysiology, in Biomedicum 2nd floor has with full temperature and CO2 control. The system allows for the use of Axion 24-well and 6-well plates. Simultaneous measurement of the signal in all the wells and electric stimulation are possible. The system is equipped with software for the quantification of spikes, bursts, and network bursts. Raw data can also be exported and analyzed elsewhere.

The 4D-Nucleofector® Core Unit and X Unit functional module support Nucleofection of various cell numbers (2 x 10^4 to 2 x 10^7) across different formats. Cell type-specific optimized protocols or recommendations can be found in Lonza's knowledge database, where the best Nucleofection conditions are outlined. Additionally, Lonza provides expertise and guidance for the handling of specific cell types, sharing valuable insights for improving transfection outcomes.

This device can be loaned from . 

FinGEEC core runs a digital slide scanning service (Pannoramic 250 FLASH III, 3DHISTECH) for both bright-field and fluorescent slides using either 20x or 40x air objectives. See ourfor a complete list of filters and options, as well as the scanning prices. Bring your slide for a free test scan! The scanner is located in Biomedicum, 5th floor. 

New instrument alert! 

FinGEEC will provide nucleofection optimization services, including equipment rental with reagents and full optimization, at the beginning of 2025.