cDNA services

GBU performs Gateway cloning to various expression vectors, and site-directed mutagenesis of our cDNA library clones.

We do cloning from the ORFeome collection, as well as from your own Gateway-compatible PCR products and plasmids to a number of destination vectors. Our selection of vectors includes a wide range of expression vectors ranging from bacterial and vertebrate expression to lentivectors. If your favourite vector is not on our list, feel free to suggest an update to our collection.

 Additionally we provide help in designing primers to create Gateway-compatible PCR products from non-Gateway-compatible templates. We can then clone the PCR products to destination vectors in two cloning steps.

The cloned products are delivered as transfection grade minipreps and glycerol stocks. Minipreps can be delivered by mail if necessary.

You can make your cloning order with the same form as the library clone orders. 

Control constructs

The following control constructs can be cloned to all destination vectors.

  • EmGFP with stop
  • EmGFP without stop
  • mCherry with stop
  • mCherry without stop
  • lacZ with stop
  • lacZ without stop
  • empty insert (the destination vectors with this insert contain no ccdb gene and can be grown in DH5alpha and stbl3 competent cells)

With our site-directed mutagenesis service, we can generate one to a few nucleotide sequence alterations to our library-derived plasmids.

The mutagenesis service fee covers the site-directed mutagenesis reaction (two tries), as well as two miniprep DNA extractions and sequencing of two mutated plasmid clones. An additional fee is charged when competent cells than standard Dh5alpha are needed (e.g. for lentiviral plasmids).

We use a Phusion HF polymerase with an error rate of 4,4 x 10-7, and while random mutations caused by the polymerase are rare, they are still possible. We confirm the mutated site by sequencing, but we do not guarantee that the construct is free of replication errors outside the mutated region.

We primarily do mutagenesis service only for the clones derived from our libraries. If you would like to have your own construct mutated, please contact us, and we will evaluate each service request case-by-case. The map and the full vector sequence are needed for the approved services. Because we can't guarantee the quality of customer's own plasmid we can't guarantee the success of the mutagenesis. We will make two trials but will need to charge them independent of the success. Failed mutagenesis is rare (<10%).


The prices have been updated and these prices are effective from March 1st 2024. 

Customer class University of Helsinki Biocenter Finland External
BP-cloning (PCR product to entry vector)      
1st item in batch 80 € 93 € 330 €
Additional items 25 € 30 € 56 €
LR-cloning (entry clone to expression vector)      
1st item in batch 65 € 77 € 293 €
Additional items 30 € 34 € 60 €
1st item in batch 150 € 173 € 584 €
Additional items 75 € 89 € 224 €
Control construct 20 € 20 € 40 €

A batch is defined as samples of one customer processed at the same time.

Examples (UH prices):
Cloning of an ORFeome clone to an expression vector: clone miniprep 36 € + LR cloning to an expression vector: 65 €, total 101 €, second clone+cloning 19 € + 30 € = 49 €.

Cloning of your own PCR product to expression vector: PCR primer design free of charge, BP cloning to entry-vector: 80 €, LR cloning to an expression vector: 65 €, total 145 €, second 25 € + 30 € = 55 €.  

Mutagenesis of library clone: Miniprep from library: 36 €, mutagenesis: 150 €, LR-cloning to expression vector: 65 €, total 251 €. 


Sample delivery

GBU also performs cloning reactions to customer-provided Gateway-compatible entry-constructs and PCR products. Please, deliver your samples to GBU sample fridge (Biomedicum Helsinki 1, 5th floor, room B505b). If you send samples by mail please use the address below:

Genome Biology Unit , 5th floor, rooms B505b ja B502a

Biomedicum Helsinki 1

Haartmaninkatu 8

P.O.Box. 63

00014 University of Helsinki 

Lenti-virus order

We collaborate with Biomedicum Virus Core (BVC) and can deliver your lenti-construct there directly. Please contact to agree on the virus production. 


If you use a clone from the GBU in your publication, please acknowledge us in the following way:

Clon­ing and mutagenesis:

“Constructs were generated by the Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland"

OR­Feome clones:

“Name of the clone (ORFeome Library; Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland)"

MGC clones:

“Name of the clone (MGC Library; Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland)"