The ORF library clones are available in Gateway-based entry vectors and we also provide cloning service into various Gateway destination vectors for Biocenter Finland research groups.
ORFeome Collaboration (full ORFs in Gateway entry vectors):
Each gene has usually two versions within the library: with and without stop codon. If you plan to make use of a vector containing C-terminal tags, select the version without stop codon.
MGC collection (cDNA clones in various vector backbones):
The MGC clones are not in Gateway-compatible vectors but we can design PCR-primers which add attB-sites to the insert and enable BP-cloning to a Gateway entry vector.
Look up your gene of interest in the libraries and copy paste the data to the following request form. If cloning to another vector is needed, please specify the vector in the form. Email the form to bch-gbu@helsinki.fi.
The clones are usually delivered as bacterial plates to customers in the University of Helsinki. If the plates can't be picked up or delivered by internal mail, we can do DNA minipreps and mail them to other Biocenter-Finland universities. DNA preps are delivered by mail. Glycerol stocks are shipped with UPS or FedEx on dry ice on the customer's expense (customer's account number required for FedEx). For plates the delivery options are pick-up and UH internal mail. The delivery time is usually within a week from order.
We always recommend to sequence the clones before the experiments. The libraries are vendor-verified but unfortunately do contain some errors. You can find the sequencing primers suitable for your clone in the info sheet we send with your order.
We can also verify the clones by Sanger sequencing with forward and reverse primers. For longer sequences this does not necessarily cover the whole gene. If you order this service, we'll align your construct against the original library clone for you and provide you the sequencing result as a link to Benchling.
Faulty clones will not be charged.
The clones from the libraries are provided only to Biocenter Finland research groups. The clones are provided free of charge but a processing fee depending on delivery format is charged.
The prices have been updated and these prices are effective from March 1st 2024.
| Service / Customer class | University of Helsinki |
Biocenter Finland |
| Plate | ||
| 1st item in batch | 20 € | 23 € |
| additional items | 15 € | 18 € |
| Glycerol stock | ||
| 1st item in batch | 20 € | 23 € |
| additional items | 15 € | 18 € |
| DNA MINIprep | ||
| 1st item in batch | 36 € | 40 € |
| additional items | 19 € | 23 € |
| DNA MIDIprep | ||
| 1st item in batch | 60 € | 67 € |
| additional items | 30 € | 37 € |
| Control construct | 20 € | 20 € |
| Clone verification by Sanger sequencing |
||
| 1st item in batch | 20 € | 22 € |
| additional items | 15 € | 17 € |
A batch is defined as samples of one customer processed at the same time.
If you use a clone from the GBU in your publication, please acknowledge us in the following way:
ORFeome clones:
“Name of the clone (ORFeome Library; Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland)"
MGC clones:
“Name of the clone (MGC Library; Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland)"