Mycoplasma contamination testing service

HTB unit offers a standardized Mycoplasma contamination testing service.

We use Lonza's MycoAlert PLUS Mycoplasma Detection Kit utilizing a luminescence-based detection of Mycoplasma-specific enzymatic activity in cell culture supernatants. The assay detects all common Mollicutes contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma).

Sample collection and preparation:

  • Allow cells to grow for a minimum of 24 hours under normal culture conditions without trypsinization or medium change prior to collecting a sample.
  • Collect 0.3-1 ml of medium from the cell culture.
  • Spin the medium at 200 x g for 5 minutes. 
  • Collect 0.2 ml of the supernatant into a 1.5 ml tube. 
  • Label the sample to include at least the sample name, freezing date, and group name.
  • Freeze the sample immediately at -80°C.
  • Bring the sample to HTB for testing.

Sample submission

  • HTB carries out the testing once a week. Inquiries regarding the next scheduled testing can be sent to Toveann Ahlnäs.
  • Service requests for Mycoplasma testing must be submitted via iLab portal. Registration is required before using the portal for the first time.
  • Please make arrangements with Toveann Ahlnäs before bringing the frozen samples to HTB.

Getting back the results

  • Testing results will be communicated to the customers within 24h after the test via iLab.


  • 30.71 € / sample for University of Helsinki
  • 32.52 € / sample for other domestic universities
  • 37.15 € / sample for other domestic and international academics 
  • 58.06 € / sample for corporate