We use Lonza's MycoAlert PLUS Mycoplasma Detection Kit utilizing a luminescence-based detection of Mycoplasma-specific enzymatic activity in cell culture supernatants. The assay detects all common Mollicutes contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma).

Sample collection and preparation:

• Allow cells to grow a minimum of 24 hours under normal culture conditions without trypsinization or changing of media prior to collecting a sample
• Collect at least 2 ml of media from the cell culture
• Spin the media sample at 1500 rpm (200 x g) for 5 minutes. Collect and freeze the supernatant immediately at -80°C.
• Label the sample to include at least the cell line information, freezing date and group name.
• Provide at least 300 µL of the sample in a 1.5 ml tube to HTB for testing.

Sample submission

• HTB carries out the testing once every 2 (two) weeks. Inquiries regarding the next scheduled testing can be sent to Toveann Ahlnäs.
• Service requests for Mycoplasma testing must be submitted via iLab portal. You would need to register before using the portal for the first time.
• Please make arrangements with Toveann Ahlnäs before bringing the frozen sample to HTB.

Getting back the results

• Testing results will be communicated to the customers within 24h after the test via iLab

Pricing

• 22.73 € / sample for domestic customers (The price is a little higher for customers abroad)