Mycoplasma contamination testing service

We use Lonza's MycoAlert PLUS Mycoplasma Detection Kit utilizing a luminescence-based detection of Mycoplasma-specific enzymatic activity in cell culture supernatants. The assay detects all common Mollicutes contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma).

Sample collection and preparation:

• Allow cells to grow for a minimum of 24 hours under normal culture conditions without trypsinization or medium change prior to collecting a sample.
• Collect 0.3-1 ml of medium from the cell culture.
• Spin the medium at 200 x g for 5 minutes. 
• Collect 0.2 ml of the supernatant into a 1.5 ml tube. 
• Label the sample to include at least the sample name, freezing date, and group name.
• Freeze the sample immediately at -80°C.
• Bring the sample to HTB for testing.

Sample submission

• HTB carries out the testing once a week. Inquiries regarding the next scheduled testing can be sent to Toveann Ahlnäs.
• Service requests for Mycoplasma testing must be submitted via iLab portal. Registration is required before using the portal for the first time.
• Please make arrangements with Toveann Ahlnäs before bringing the frozen samples to HTB.

Getting back the results

• Testing results will be communicated to the customers within 24h after the test via iLab.

Pricing

• 30.71 € / sample for University of Helsinki
• 32.52 € / sample for other domestic universities
• 37.15 € / sample for other domestic and international academics 
• 58.06 € / sample for corporate