Functional genomics screening

RNAi screening

HTB supports automated screening of cells cultured in 96-, 384- or 1536-plate formats and can be applied to multiple investigator-initiated applications throughout the academic community.

The core maintains the Ambion Silencer Select V4-library and houses instruments needed to process a large amount of samples in high throughput fashion. Our platforms enable genome-wide, sub-library or candidate gene screens. We support both plate reader assays (luminescence, fluorescence, absorbance) and high content microscopy. We also provide expertise in assay development and optimization.

The Ambion Silencer® Select Human siRNA Library V4

The library is organized into three main groups:  Druggable genome, the genome extension set, and the druggable genome extension set. The library consists of three individual siRNAs per target.

HTB Ambion siRNA library composition

 

The library is available to be screened on 384-well plates

HTB Ambion siRNA library details

 

Custom library

We can also help you with designing and ordering custom siRNA or miRNA libraries. Please inquire.

How to get started?

All projects are customized to a researchers needs. A typical workflow for siRNA screening is shown here.

If you are interested in any of our services for application of your project, please complete the project inquiry form and we will contact you.

HTB RNAi screening workflow