High throughput flow cytometry

 

FIMM high throughput flow cytometry Tanja

 

Flow cytometry -based drug screening assay provides cell population-specific drug response data.

  • The platform allows to study the activity and mechanism of action of approved and investigational drugs and to learn about cell vulnerabilities and drug resistance mechanisms. 
  • Samples are typically biobanked frozen or freshly isolated mononuclear cells from hematological malignancies or cell lines. 
  •  We use drugs or drug candidates and cell surface markers of your choice. See contact details below.
  • Oncology collection QA1B has 40 clinically relevant drugs and combinations in seven doses on a 384-well plate. QA1B is designed for acute myeloid leukemia and acute lymphoblastic leukemia. Other drug collections are available.
  • Optimized antibody panels to detect main cell types of AML and ALL contain up to 9 cell surface markers and viability dyes.
  • Efficient and economical use of detection antibodies is achieved using ECHO acoustic dispenser for accurately transferring small volumes of reagents and other lab automation for antibody staining.
  • Drug response data is reported using a web-based service (TDB).

The chart below illustrates the workflow and differences between the classical DSRT, in which all cells in a well are evaluated as a single unit, and flow DSRT, in which each cell in each well produces a data point for each marker/antibody, thus allowing to reveal population-specific drug responses:

FICAN 2018 DSRT scheme

 

With technical questions concerning HT flow cytometry contact Tanja Ruokoranta.