High-Content Screening

 

3I Marianas (3I intelligent Imaging Innovations) is a fluorescence microscope designed for imaging live cells with multiple colours and techiques. Available imaging techniques are wide-field, TIRF and spinning disk confocal. There are nine light sources (5 LEDs and 4 lasers) and fast switching times between imaging techniques and light sources so that one can make almost any combination of videos. Additionally it is possible to do photomanipulation experiments (FRAP an photo activation) . Microscope has a motorised stage which can be utilized for multiposition imaging or image plates.

Room 2018a

Technical details

See LMU wiki.

 

Cell-IQ is a continuous cell culturing platform for cell lines, primary cells, co-cultures and monolayer tissue models. It identifies, visualizes and records in real time all changes in morphology and physiology down to the single cell during an incubation and translates the images into graphical outputs. We have two units. One with phase contrast and 3 channel fluorescence immaging and the other with only phase contrast imaging.

Room 2037d

Technical details

See LMU wiki.

 

SP5II HCS is a fully motorized inverted point scanning confocal. It is especially good for high resolution imaging for live samples without blur from out of focus signal. Scanner has two extra sensitive low noice detectors for dim samples and faster imaging.

Microscope has a motorised stage for multipoint or tile imaging and the system has a Leica Matrix software for high content imaging from plates. You can also aqcuire cross section images.

 

Room 2036b

Technical details and recommended well-plates

See LMU wiki.

MolecularDevices Nano can be used for automated imaging of well-plates and slides (using an adapter). It has several built-in image analysis algorithms that can use two channels. The data format is proprietary. TIFF export is planned for CRX software version 2 (due in spring 2018).

Room 2037d

Technical details and instructions

See LMU wiki.

 

Cellomics CellInsight is a compact plate reader. It accepts most well-plate types and there is an option to  design custom sample geometries for e.g. micro arrays. Due to the lack of CO2 chamber and heating the system does not suit live cell work.

For image analysis, we recommend CellProfiler. We do have a license for Thermo Bio-Applications for running image analysis either during scanning, or later on (disk-based rescan), but this software is only available on the instrument PC.

Our CellInsight is not working perfectly. There are random imaging errors where channels are either switched or missing altogether. According to Thermo, spare parts are no longer available for this instrument. You can find a more reliable CellInsight at BIU.

Room 2036a

Technical details

See LMU wiki.