Instruments and services

Located at Biomedicum flow cytometry unit.

The BD Accuri C6 Flow Cytometer analyser can be used after training. The instrument has a blue (488 nm) and a red (640 nm) laser. Simultaneously you can analyse with 4 different fluorescent and 2 scatter parameters.

The selectable laser module allows 3 different filter configurations: 3 blue and 1 red (standard setting), 2 blue and 2 red or 4 blue. User changeable optical filters available are: 530/30 nm, 585/40 nm, 610/20 nm, 670 nm (LP), 675/25 nm and 780/60 nm. There are also optional 99 % attenuation filters for each detector.

Located at Biomedicum flow cytometry unit.

The SH800Z Cell Sorter can be used after training. The instrument has two lasers: Blue (488 nm) and Yellow-Green (561nm).
You can analyze up to 6 fluorescence and 2 scatter parameters.
The instrument is equipped with PMTs with optimal filters for detecting fluorescent proteins.
Three differnt nozzle chips are avilable, 70µm, 100µm and 130µm nozzle chips with automatic alignment for sorting. Cells can be sorted into 12 x 75 mm tubes or 15 ml falcon tubes with two way sort. It is also possible to sort on to various plates up to 384 well format (single or multiple cells/well).

The sort chips combined with the possibility to have your own sample line ensures pure sorts.

SH800z Operators Guide

SH800z General operation

Detectors

Optical Filters

Located at Biomedium and Viikki sorting flow cytometry units.

The BD FACS Aria II Flow Cytometer sorter can be used after training. Cells can be sorted into 1 ml tubes, 12 x 75 mm tubes, 15 ml falcon tubes, into various plates (single or multiple cells/well), or onto microscope slides. Aria is capable of simultaneous 4-way sorting. Sample temperature can be adjusted during analysis and sorting (+4°C - +37°C). FACSAria uses BD FACSDiVa Software v 8.0.

FACSAria User Guide

Biomedicum: 12 detectors: FSC, SSC, 5 fluorescence detectors from the 488 mn, 3 fluorescence detectors from the 633 nm, and 2 fluorescence detectors from the 405 nm laser.

Detectors

Optical Filters

Viikki: 11 detectors: FSC, SSC, 5 fluorescence detectors from the 488 mn, 2 fluorescence detectors from the 633 nm, and 2 fluorescence detectors from the 375 nm laser.

Specifications

Located at Biomedicum flow cytometry unit.

BD Influx Cell sorter is avilable only with an operator.

The instrument has five lasers: Violet (405nm), Blue (488 nm), Yellow-Green (561nm), Red (640nm) and Near Infrared (785nm). You can analyze up to 18 fluorescence and 2 scatter parameters. Nozzles sizes available: 70, 86, 100 and 140 µm. Cells can be sorted into 0,5-50 ml tubes and onto various plates, up to 384-well plates (single or multiple cells/well), or onto microscope slides. The system can be setup for simultaneous 6-way sorting (into tubes).

Located at Biocenter flow cytometry unit.

The BD LSR II Flow Cytometer analyser can be used after training. BD LSR II has two lasers: blue 488 nm and red 633 nm. You can analyze up to 6 fluorescence and 2 scatter parameters simultaneously. Samples can be analyzed with 3 different flow rates: low (12 µL/min), medium (35 µL/min) and high (60 µL/min). Flow rate can also be adjusted with sample fine adjustment (6-24, 17.5-70 and 30-120 µL/min, respectively). LSR II uses BD FACSDiva Software v 8.0.

BD LSR II User's Guide

Detectors

Optical Filters

Located at Biocenter flow cytometry unit.

The BD LSRFortessa Flow Cytometer analyser can be used after training. BD LSRFortessa has 4 lasers: violet 405 nm, blue 488 nm, yellow green 561 nm and red 640 nm. You can analyze up to 16 fluorescence and 2 scatter parameters simultaneously. Samples can be analyzed with 3 different flow rates: low (12 µL/min), medium (35 µL/min) and high (60 µL/min). Flow rate can also be adjusted with sample fine adjustment (6-24, 17.5-70 and 30-120 µL/min, respectively). BD LSRFortessa uses BD FACSDiva Software v 8.0

BD LSRFortessa Cell Analyzer User’s Guide

Detectors

Optical Filters

Located at Biomedicum flow cytometry unit.

The Beckman Coulter Flow Cytometer analyser can be used after training. The instrument has a blue (488 nm) and a red (638nm) laser. Simultaneously you can analyse with 6 different fluorescent and 2 scatter parameters.

Optical filters available are: 525/40 nm, 575/30 nm, 620/30 nm, 695/30 nm, 755 (LP) nm and on the red side 660/20 nm.

Lazers and Detectors

Manual

Located at Biomedicum flow cytometry unit.

You can run two samples at a time with preinstalled programs for different applications. Use C tubes for dissociation of tissues to obtain single-cell suspension and M tubes for homogenization of tissues or cells to isolate subcellular material. Tubes are not provided. No training necessary, but permission and reservation is required.

gentleMACS Dissociator

MACS tissue Dissociation kits.