ORF libraries

GBU's reagent collection contains a library of open reading frames (ORFeome collaboration) consisting of about 18 600 fully verified clones, and a collection of full-length cDNAs (MGC collection) consisting of about 29 000 clones.

The ORF library clones are available in Gateway-based entry vectors and we also provide cloning service into various Gateway destination vectors for Biocenter Finland research groups.
ORFeome collection

ORFeome Collaboration (full ORFs in Gateway entry vectors):

  • human clones      16934
  • mouse clones       1758

Each gene has usually two versions within the library: with and without stop codon. If you plan to make use of a vector containing C-terminal tags, select the version without stop codon.  
MGC collection

MGC collection (cDNA clones in various vector backbones):

  • human clones      20 945
  • mouse clones       8290

The MGC clones are not in Gateway-compatible vectors but we can design PCR-primers which add attB-sites to the insert and enable BP-cloning to a Gateway entry vector. 
Order and delivery

Look up your gene of interest in the libraries and copy paste the data to the following request form. If cloning to another vector is needed, please specify the vector in the form. Email the form to bch-gbu@helsinki.fi.

The clones are usually delivered as bacterial plates to customers in the University of Helsinki. If the plates can't be picked up or delivered by internal mail, we can do DNA minipreps and mail them to other Biocenter-Finland universities. DNA preps are delivered by mail. Glycerol stocks are shipped with UPS or FedEx on dry ice on the customer's expense (customer's account number required for FedEx). For plates the delivery options are pick-up and UH internal mail. The delivery time is usually within a week from order. 
Price

The clones from the libraries are provided only to Biocenter Finland research groups. The clones are provided free of charge but a packaging fee of 20 € is charged for delivered plates. For minipreps an additional 20 € is charged. Glycerol stocks are available for 15 € for the first clone in the order and 10 € for the subsequent ones. 

Control constructs: 20 € / miniprep. 
Citation

If you use a clone from the GBU in your publication, please acknowledge us in the following way:

OR­Feome clones:

“Name of the clone (ORFeome Library; Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland)"

MGC clones:

“Name of the clone (MGC Library; Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland)"