Custom shRNA cloning

Design and order your oligos according to by Addgene. Note that the ends of the oligos must be exactly as detailed in the protocol for the cloning to work. We can verify your oligo sequences if needed. Deliver the oligos to us and we can proceed with the cloning. 

Oligonucleotide ordering tips: Standard de-salted oligos (usually the most inexpensive synthesis) are sufficient for cloning. If not already resuspended, dilute each oligo to 100 μM in DNAse-free water.

The shRNA oligos are annealed and cloned to TRC library vector (), a lentivector with puromycin resistance for selection in mammalian cells. The clones are verified with Sanger sequencing, and the customer will receive a DNA miniprep of each of the the clones. Bacterial glycerol stocks will also be prepared and can be picked up from GBU if needed. 

The clones can be submitted directly to for virus production. Please contact for more information and orders. 

A batch is defined as samples of one customer processed at the same time. Control constructs cost 20 € /miniprep. 

If you use a clone from the GBU in your publication, please acknowledge us in the following way:

“shRNA constructs were generated by the Genome Biology Unit supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland"

Please also cite the creators of the vector backbone of your construct: ()