Metabolomics /Infrastructures /

Helsinki University Lipidomics Unit (HiLIPID)

Welcome to Helsinki University Lipidomics Unit (HiLIPID) – Research Infrastructure of HiLIFE – Helsinki Institute of Life Science.

HiLIPID is a co-operational venture of the Faculty of Biological and Environmental Sciences and Faculty of Medicine consisting of two lipidomics laboratories. One of the laboratories located at the Molecular and Integrative Biosciences, Viikki Campus, is run by adjunct professor Reijo Käkelä (Functional Lipidomics Group), and the other laboratory at Institute of Biomedicine, Meilahti Campus, is run by adjunct professor Pentti Somerharju (The Somerharju Lipid Group).

HiLIPID is an academic research and service facility, which provides services subject to charge for research groups, companies and authorities. We are serving a range of different research fields in life sciences; customers are from medical, biological, environmental, and veterinary sciences.

Our strengths are mass spectrometry-based lipidomics and gas chromatography of fatty acids followed by multivariate data analysis. We have experience in metabolic studies utilizing labeled precursors.

Services
Instruments
Fees and Analysis
Contact

Services

ShimadzuGC-FID, HiLIPID, University of Helsinki
  • Sample preparation and derivatization by trained users or as service
  • Instrument use by trained users with specialist support and maintenance
  • Full analytical service from sample preparation to data treated by multivariate statistics
  • Other tailored combinations of user and specialist work

Instruments

ShimadzuGC-MSD, HiLIPID, University of Helsinki
  • Agilent 6490 Triple Quad LC/MS with 1290 Infinity LC
  • Waters Quattro Premier with Acquity LC
  • Shimadzu GCMS-QP2010 Ultra, GC-MSD
  • Shimadzu GC-2010 Plus system, GC-FID
  • Camag HPTLC system

Fees and Analysis

Agilent 6490 Triple Quad ESI-MS/MS, HiLIPID, University of Helsinki

HiLIPID provides various services ranging from full analysis of lipids to supported renting of the instruments. The service list describes the typical services we provide, however, please ask a quotation for your customized service.

Please note that specific long-term mass spectrometric or chromatographic full services (including metabolic studies using labeled lipid precursors, analyses of signaling lipids or other services that require reserving of machinery for a longer period for one application) should always be based on written service agreements with case-specific prices. The fees below do not take into account discounts that may follow from collaboration in terms of research, education or method development. Moreover, we offer multivariate statistical analyses.

Service

University of Helsinki research groups

Other customers
(+ VAT 24%)

Folch lipid extraction (tissue, cell or fluid sample); service

17 € / sample

42 € / sample

Preparing methyl ester derivatives for GC of fatty acids; service

17 € / sample

42 € / sample

HPTLC of lipid classes; rental at site

36 € / plate (8 samples)

72 € / plate (8 samples)

HPTLC analysis of lipid classes; full service

110 € / plate (8 samples)

240 € / plate (8 samples)

GC-FID of fatty acids; chromatogram and numeric data only for trained users performing data analysis by themselves

24 € / sample

48 € / sample

GC-FID fatty acid analysis; full service

54 € / sample

108 € / sample

GC-MSD verification of fatty acid structures; service

95 € / sample (one required for each sample type)

190 € / sample (one required for each sample type)

Triple quadrupole ESI-MS/MS of lipids with syringe infusion; rental at site

95 € / hour

180 € / hour

Triple quadrupole ESI-MS/MS of basic lipid package* with syringe infusion; mass spectra and numeric data only

72 € / sample

144 € / sample

Triple quadrupole ESI-MS/MS of basic lipid package* with syringe infusion; full service

110 € / sample

220 € / sample

*Basic lipid package includes the analysis of lipid classes depicted in the table below. We also provide analyses of other lipids upon request!

Analysis we offer

Analysis of specific lipid class is included provided its amount in the sample enables its detection with the machinery available.

BASIC LIPID PACKAGE

Scan type

Phosphatidylcholine (PC) and Lyso-PC

Precursor Ion 184

Phosphatidylethanolamine (PE) and Lyso-PE

Neutral Loss 141

Phosphatidylserine

Neutral Loss 87 / Neutral Loss 185

Phosphatidylinositol

Precursor Ion 241

Sphingomyelin

Precursor Ion 184

Cholesterol esters

Precursor Ion 369

Triacylglycerides (TAG)

MS Positive as NH3+ adducts

UPON REQUEST

 

Phosphatidylethanolamine plasmalogens

MS Negative & confirmatory Precursor Ion scans for 364 (16:0p), 390 (18:1p), and 392 (18:0p)

Ceramides

Precursor Ion 264

Hexocylceramides

Precursor Ion 264

Sulphatides

Precursor Ion 97

Diacylglycerides (DAG)

MS Positive as NH3+  adducts

Monoacylglycerides (MAG)

MS Positive as NH3+  adducts

Contact

Viikki Campus, University of Helsinki

Please contact: hilipid-info@helsinki.fi

R&D Laboratory coordinator:
Minna Holopainen, MSc
minna.holopainen@helsinki.fi

R&D Laboratory coordinator:
Hanna Ruhanen, MSc
hanna.ruhanen@helsinki.fi

Principal investigator (unit head):
Adjunct professor Reijo Käkelä
reijo.kakela@helsinki.fi
+358 (0) 504486506

Principal investigator:
Adjunct professor Pentti Somerharju
pentti.somerharju@helsinki.fi
+358 (0) 504482168

Laboratory technician:
Sanna Sihvo

 

Address

Faculty of Biological and Environmental Sciences
Helsinki University Lipidomics Unit
Viikki Campus
Biocenter 3, 2nd floor
(Viikinkaari 1)
PO BOX 65
00014 University of Helsinki
FINLAND