HiLIPID service laboratory is located at the Viikki campus (Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences) and is run by adjunct professor Reijo Käkelä with Dr Minna Holopainen, Dr Hanna Ruhanen and Dr Andrew House, having adjunct professor Pentti Somerharju as senior adviser.
HiLIPID is an academic research and service facility, which provides services subject to charge for research groups, companies and authorities. We are serving customers from the fields of environmental, ecological, basic biosciences, veterinarian and biomedical research.
Services have been provided since 2014, based on the experience of nearly 40 years in lipid analytics. Our strengths are mass spectrometry-based lipidomics and gas chromatography of fatty acids followed by multivariate data analysis. We have experience in metabolic studies utilizing labeled precursors. Recently, lipid mediator analysis was added to our service catalog. We have obtained a high average score in HiLIFE research infrastructure user survey, and performed especially well in quality and staff support.
HiLIPID provides various services ranging from full analysis of lipids to supported renting of the instruments. The service list describes the typical services we provide, however, please ask a quotation for your customized service.
Note that specific long-term mass spectrometric or chromatographic full services (including metabolic studies using labeled lipid precursors, analyses of lipid mediators or other services that require reserving of machinery for a longer period for one application) should always be based on written service agreements with case-specific prices. Moreover, we offer multivariate statistical analyses. Please contact us for more information!
| Service | University of Helsinki research groups |
External - academic (+VAT 25.5%) |
External (+VAT 25.5%) |
| Fatty acid analysis with gas chromatography (GC) | |||
| Preparing methyl ester derivatives for GC of fatty acids; service | 15 €/sample | 29 €/sample | 58 €/sample |
| GC-FID of fatty acids; chromatogram and numeric data; only for trained users performing data analysis by themselves* | 30 €/sample | 42 €/sample | 69 €/sample |
| GC-FID fatty acid analysis; service | 52 €/sample | 91 €/sample | 176 €/sample |
| GC-MSD verification of fatty acid structures; service | 111 €/sample (one required for each sample type) | 188 €/sample (one required for each sample type) | 350 €/sample (one required for each sample type) |
| Lipid analysis with mass spectrometry (MS) | |||
| Folch lipid extraction (tissue, cell or fluid sample); service | 15 €/sample | 29 €/sample | 58 €/sample |
| Triple quadrupole ESI-MS/MS of lipids with syringe infusion; rental at site | 97 €/hour | 110 €/hour | 209 €/hour |
| Triple quadrupole ESI-MS/MS of basic lipid package** with syringe infusion; mass spectra and numeric data only; only for trained users performing data analysis by themselves | 97 €/sample | 110 €/sample | 165 €/sample |
| Triple quadrupole ESI-MS/MS of basic lipid package** with syringe infusion; service | 120 €/sample | 144 €/sample | 250 €/sample |
| Triple quadrupole LC-MS/MS of basic lipid package**; mass spectra and numeric data only; only for trained users performing data analysis by themselves | 66 €/sample | 80 €/sample | 165 €/sample |
| Triple quadrupole LC-MS/MS of basic lipid package**; service | 94 €/sample | 139 €/sample | 250 €/sample |
| Lipid class analysis hy high-performance thin layer chromatography (HPTLC) | |||
| HPTLC of lipid classes; rental at site | 67 €/plate (8 samples) | 100 €/plate (8 samples) | 140 €/plate (8 samples) |
| HPTLC analysis of lipid classes; full service | 115 €/plate (8 samples) | 175 €/plate (8 samples) | 285 €/plate (8 samples) |
| Lipid mediator analysis with mass spectrometry | |||
| Lipid mediator extraction; service | 26 €/sample | 42 €/sample | 71 €/sample |
| LC-MS/MS analysis of lipid mediators; service | 127 €/sample | 161 €/sample | 240 €/sample |
* additional fee 3 €/sample if sample concentration is required
** Basic lipid package includes the analysis of lipid classes depicted in the table below. We also provide analyses of other lipids upon request!
Analysis of the lipid species of a specific lipid class is included provided its amount in the sample enables its detection with the machinery available.
| LIPID CLASS | Scan mode |
| Phosphatidylcholines (PC) and LPCs | Precursor Ion 184 |
| Phosphatidylethanolamines (PE) and LPEs | Neutral Loss 141 |
| Phosphatidylserines (PS) | Neutral Loss 87 / Neutral Loss 185 |
| Phosphatidylinositols (PI) | Precursor Ion 241 |
| Sphingomyelins (SM) | Precursor Ion 184 |
| Cholesteryl esters (CE) | Precursor Ion 369 |
| Triacylglycerols (TG) | MS Positive as NH3+ adducts |
| UPON REQUEST | |
| Phosphatidylethanolamine plasmalogens (PE P) | MS Negative & confirmatory Precursor Ion scans for 364 (16:0p), 390 (18:1p), and 392 (18:0p) |
| Phosphatidylglycerols (PG) | Neutral Loss 189 |
| Phosphatidic acids (PA) | Neutral Loss 115 |
| Ceramides (Cer) | Precursor Ion 264 |
| Hexosylceramides (HexCer) | Precursor Ion 264 |
| Sulfatides (SHexCer) | Precursor Ion 97 |
| Diacylglycerols (DG) | MS Positive as NH3+ adducts |
Please contact: hilipid-info@helsinki.fi
R&D, Postdoctoral researcher:
Minna Holopainen, PhD
minna.holopainen@helsinki.fi
R&D, Postdoctoral researcher:
Andrew House, PhD
andrew.house@helsinki.fi
R&D, Postdoctoral researcher:
Hanna Ruhanen, PhD
hanna.ruhanen@helsinki.fi
Principal investigator (unit head):
Adjunct professor Reijo Käkelä
reijo.kakela@helsinki.fi
+358 (0) 504486506
Laboratory technician:
Sanna Sihvo
sanna.sihvo@helsinki.fi
Address
Faculty of Biological and Environmental Sciences
Helsinki University Lipidomics Unit
Viikki Campus
Biocenter 3, 2nd floor
(Viikinkaari 1)
PO BOX 65
00014 University of Helsinki
FINLAND