Viikki Metabolomics Unit (ViMU)

Welcome to Viikki Metabolomics Unit (ViMU) – Research Infrastructure of HiLIFE – Helsinki Institute of Life Science.

ViMU is focused on plant metabolomics, but additionally unit offers analytical services for microbial applications and for pharmacokinetics and novel drug development. The unit offers both targeted and non-targeted analysis using MS-based techniques: UPLC-PDA-QTOF/MS, UPLC-QTRAP/MS and GC-QQQ/MS.

The laboratory is located at Viikki Campus. It is part of Organismal and Evolutionary Biology Research Programme (OEB) of the Faculty of Biological and Environmental Science. ViMU is run by Professor Teemu Teeri and laboratory coordinator Nina Sipari.

ViMU offers analytical services subject to charge for academic research groups, mainly for Finnish Plant Research community, but also for companies.

Publications of ViMU can be found in research database Tuhat.

 

  • Full analytical service from sample preparation to RAW-data
  • Sample prices include: sample preparation including derivatization (if needed) and internal standards (ISTD), QC’s and blank runs.
  • Targeted analysis: ISTD’s, STD’s and QC’s (calibration curve) and quantification calculations included to the price.
  • Non-targeted analysis: Basic multivariate statistical analysis (PCA, PLS-DA, OPLS-DA) included to the price, identification of (novel) metabolites etc. not included, ask N. Sipari for details.
  • Other customized analysis and data processing contact N.Sipari for details.

Non-Targeted metabolite profiling/metabolic fingerprinting

  • UPLC-QTOF/MS analysis
  • GC-QQQ/MS; small biomolecules (sugars and sugar phosphates, organic acids, amino acids etc.)

Targeted, quantitative analysis

  • UPLC-QTRAP/MS (MRM)
  • GC-QQQ/MS (MRM)

Exact mass measurements (MS, MS/MS, MSe)

  • UPLC-QTOF/MS

Ap­plic­a­tions

  • Analysis of plant, microbe, algae, cyanobacteria metabolites (both primary and secondary metabolites): standard assays available: phytohormones, central carbon & nitrogen metabolism, plant sec. metabolites
  • Metabolic profiling/phenotyping (plant lines, treatments, biomarkers)
  • Pharmaceutical area (synthesis products, pharmacokinetics, metabolism)
  • Customized analysis (contact N.Sipari for more detail)
  • Waters Acquity UPLC-PDA-Synapt G2 HDMS/QTOF
  • AbSciex UPLC-QTRAP 6500+
  • Agilent GC-QQQ/MS 7000

The research infrastructure is supported by University of Helsinki (HiLIFE, Faculty of Biological and Environmental Sciences) and Biocenter Finland.

The service list below describes the most typical services provided by ViMU. For customized service and method development and industrial customers, please ask a quotation.

Note that specific long-term mass spectrometric or chromatographic full services (including metabolic studies using labeled lipid precursors, analyses of signaling lipids or other services that require reserving of machinery for a longer period for one application) should always be based on written service agreements with case-specific prices.

The academic prices (excl. VAT) are listed below and include only consumables and maintenance.

Service

€/sample

Instrument

Sample price includes

Non-targeted analysis

50

UPLC-QTOF or GC-QQQ/MS**

Sample, ISTD and QC runs*

Targeted analysis

80

UPLC-QTRAP (MRM) or GC-QQQ/MS (MRM) **

Sample, ISTD, QC and STD runs (calib. curve)

Customized analysis

 

GC-QQQ/MS, UPLC-QTOF, UPLC-QQQ/QTRAP

 

Data analysis

50€/h

 

 

* Data provided as RAW, NetCDF or MzXML format or txt, csv or xlsx-files. ** Primary metabolites

Different analysis require different amounts of sample, usually around 10-100mg (FW).

Sample tubes: preferably in 1.5 or 2.0 ml eppendorf tubes.

Sample Collections: Quenching in liquid nitrogen immediately after collection and weighing.

Sample storage: at -80°C immediately after the sample collection.

Sample delivery: frozen samples on dry ice if delivered by post/DHL etc. and in liquid nitrogen if delivered personally within Viikki campus.

Sample list: Please send us the sample list (nina.sipari@helsinki.fi) in an excel file format with all the relevant details i.e., sample names, (fresh/dry) weights, genotype, phenotype, type of sample, age, treatment information, time series data, plus additional information. Please, use running numbers (1, 2, 3…etc.) if possible for naming the samples.

Types of samples and requirements per sample: Contact laboratory coordinator N. Sipari for more details.

Please contact:

R&D Laboratory coordinator
Nina Sipari
nina.sipari@helsinki.fi
+358 (0) 50 415 0337

Principal investigator (Head of the unit)
Professor Teemu Teeri
teemu.teeri@helsinki.fi
+358 (0) 50 4480 480

Location and Shipping Address
Viikki Metabolomics Unit
Nina Sipari
Viikki Campus
Biocenter 2, 1st floor (office 1105 or MS-lab 1079)
(Viikinkaari 5 E)
PO BOX 65
00014 University of Helsinki
FINLAND