FIMM Meta Services

All open access services include short discussion and consultation for suitable method, sample preparation/delivery guidelines and prices and delivery of the concentration or peak area data in excel report and transfer of raw files upon customer request. Paid services do not include experiment design, statistical analysis, creating publication images or result interpretation. The analyses are always monitored with inhouse Quality control and missing values for certain metabolites or in certain samples can appear in all methods.
Services

Identification and within run relative quantitation of more than 470 metabolites using high resolution orbitrap mass spectrometer.

The exact number of detected metabolites is dependent on sample matrix and sample amount.

Link to comprehensive compound list.

Throughput approximately 150 samples per week.

Collaboration article DOI:10.1126/sciadv.adf7119, check publication list for additional publications.

Sample types (minimum amount)

Biofluids

    Serum (110 µl)

    Plasma (110 µl)

    CSF (15 µl)

    Sweat

    Tears

    Whole blood

Tissues (15 mg)

    Mouse heart (0.5 mg)

    Spleen (20 mg)

    Brain

    Muscle

    Cecum

    Gut

    Skin

    Mucus

Others

    Feces

    Cells (1x10e6)

    Organoids

    Spheres

    Bacteria

    Extracellular vesicles (10 µl)

    Cell media

Quantitative LC-MS concentration measurement using internal standard with MRM

Link to comprehensive compound list.

Suitable for tissues, cells, and biofluids (https://doi.org/10.3390/metabo8030044, check publication list for additional publications). 

 

Sample types (minimum amount)

Biofluids (110 µl)

Tissues (15 mg)

Cells (1x10e6)

Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.

Compounds

Tetrahydrofolate

Dihydrofolate

5,10-methenyl tetrahydrofolate

5,10-methylene tetrahydrofolate

5-formyl tetrahydrofolate

5-methyl tetrahydrofolate

NOTE: Folate samples must be stored in amber coloured vials.

Sample types (minimum amount)

Whole blood (60 µl)

Tissues (20 mg)

Cells (1x10e6)

CSF (15 µl)

Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.

https://doi.org/10.3390/metabo10030103

Compounds.

Glycine

Alanine

Serine

Aspartate

Glutamine

Glutamate

Pyruvate

Lactate

Fumarate

Succinate

Malate

Oxoglutarate

Cis-aconitate

Citrate

Isocitrate

Sample types (minimum amount)

Biofluids (110 µl)

Tissues (15 mg)

Cells (1.5x10e6)

Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.

Compounds.

2-hydroxybutyric acid

3-hydroxybutyric acid

2-ketobutyric acid

3-ketobutyric acid (Acetoacetic acid)

Sample types (minimum amount)

Biofluids (110 µl)

Identification and absolute quantitation with internal standards using high resolution orbitrap mass spectrometer.

Throughput approximately 150 samples per week.

Compounds.

Acetic Acid

Propionic Acid

Butyric/Isobutyric Acid

Valeric Acid

Isovaleric Acid

2-Methylvaleric Acid

3-Methylvaleric Acid

Sample types (minimum amount)

Biofluids

    Serum (110 µl)

    Plasma

    Whole blood

Tissues

    Cecum (20 mg)

Others

    Feces (10 mg)

    Cells

    Organoids

    Spheres

    Bacteria

    Extracellular vesicles

Sample types (minimum amount)

Whole blood (60 µl)

Analysis of thousand lipid molecular species in a single method using the Lipidyzer platform.

More details doi: 10.1021/acs.analchem.1c02826

Link to compound list.

Sample types (minimum amount)
Biofluid (25 µl) 
Tissues (15 mg)
Cells (1x10e6), Nanovesicles (25ul)

 

 

Isotope enrichment analysis and metabolic flux analysis of central carbon metabolism, glucose, NADolome, glutamine and other pathways using 13C-, 15N- and dH-tracers.

Collaboration article https://doi.org/10.1016/j.redox.2022.102416, check publication list for additional publications.

Requires consultation and collaboration.

Sample types (minimum amount)

Biofluids (10µl)

Tissues

Cells (30000)

Organoids

Spheres

Extracellular vesicles

Select metabolites from 450+ list. Direct orders via iLAB or for further information contact fimm-metabolomics@helsinki.fi.

We are open to develop via collaboration new metabolomics analytical methods both for precision metabolomics and research purposes. Contact the head of the unit.

We are open to develop via collaboration new metabolomics analytical methods both for precision metabolomics and research purposes. Contact the head of the unit.



As an example, these compounds have been quantitated from customer samples.

Riboflavin

GABA

L-Cystine

Pyruvate

Myo-inositol

Cortisol

Biotin

3,3',5'-Triiodothyronine

Glutathione

GSSG

 

We offer via collaboration help for metabolomics/lipidomics data analysis.

Contact the head of the unit.

We are open for consulting patient and clinical metabolomics work, teaching, seminar and conference talks. 

Contact the head of the unit.

Multiomics workflows:

HiLIFE bioinformatics multi-omics data analysis service workflow will be launched 2025 ->

further information: anni.nieminen@helsinki.fi

 

HiLIFE single-cell omics service workflow will be launched 2025 ->

further information: anni.nieminen@helsinki.fi

 
General info

The compounds measured with the methods are listed under each method. If the sample amount is very low and/or the matrix is new the detectability of the compounds of interest must be checked with a test sample before running the actual samples.

Results are delivered in an excel file. Fluxomics and 450+ metabolites methods are relatively quantitative and the results are given as peak areas. In all other quantitative methods the results are given in µM or other suitable concentration unit.

The raw files are also available if required.

Leftover samples are discarded unless otherwise agreed.

Raw data and all processing files are stored for 2 years after reporting.