FIMM Meta Services

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Services

Identification and within run relative quantitation of more than 470 metabolites using high resolution orbitrap mass spectrometer.

The exact number of detected metabolites is dependent on sample matrix and sample amount.

Link to comprehensive compound list.

Throughput approximately 150 samples per week.

Collaboration article DOI:10.1126/sciadv.adf7119, check publication list for additional publications.

Sample types (minimum amount)
Biofluids
    Serum (110 µl)
    Plasma (110 µl)
    CSF (15 µl)
    Sweat
    Tears
    Whole blood
Tissues (15 mg)
    Mouse heart (0.5 mg)
    Spleen (20 mg)
    Brain
    Muscle
    Cecum
    Gut
    Skin
    Mucus
Others
    Feces
    Cells (1x10e6)
    Organoids
    Spheres
    Bacteria
    Extracellular vesicles (10 µl)
    Cell media

Quantitative LC-MS concentration measurement using internal standard with MRM

Link to comprehensive compound list.

Suitable for tissues, cells, and biofluids (https://doi.org/10.3390/metabo8030044, check publication list for additional publications). 

 

Sample types (minimum amount)
Biofluids (110 µl)
Tissues (15 mg)
Cells (1x10e6)

Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.

Compounds
Tetrahydrofolate
Dihydrofolate
5,10-methenyl tetrahydrofolate
5,10-methylene tetrahydrofolate
5-formyl tetrahydrofolate
5-methyl tetrahydrofolate

NOTE: Folate samples must be stored in amber coloured vials.

Sample types (minimum amount)
Whole blood (60 µl)
Tissues (20 mg)
Cells (1x10e6)
CSF (15 µl)

Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.

https://doi.org/10.3390/metabo10030103

Compounds.
Glycine
Alanine
Serine
Aspartate
Glutamine
Glutamate
Pyruvate
Lactate
Fumarate
Succinate
Malate
Oxoglutarate
Cis-aconitate
Citrate
Isocitrate

Sample types (minimum amount)
Biofluids (110 µl)
Tissues (15 mg)
Cells (1.5x10e6)

Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.

Compounds.
2-hydroxybutyric acid
3-hydroxybutyric acid
2-ketobutyric acid
3-ketobutyric acid (Acetoacetic acid)

Sample types (minimum amount)
Biofluids (110 µl)

Identification and absolute quantitation with internal standards using high resolution orbitrap mass spectrometer.

Throughput approximately 150 samples per week.

Compounds.
Acetic Acid
Propionic Acid
Butyric/Isobutyric Acid
Valeric Acid
Isovaleric Acid
2-Methylvaleric Acid
3-Methylvaleric Acid

Sample types (minimum amount)
Biofluids
    Serum (110 µl)
    Plasma
    Whole blood
Tissues
    Cecum (20 mg)
Others
    Feces (10 mg)
    Cells
    Organoids
    Spheres
    Bacteria
    Extracellular vesicles

Sample types (minimum amount)
Whole blood (60 µl)

Analysis of thousand lipid molecular species in a single method using the Lipidyzer platform.

More details doi: 10.1021/acs.analchem.1c02826

Link to compound list.

Sample types (minimum amount)
Biofluid (25 µl) 
Tissues (15 mg)
Cells (1x10e6), Nanovesicles (25ul)

 

 

Isotope enrichment analysis and metabolic flux analysis of central carbon metabolism, glucose, NADolome, glutamine and other pathways using 13C-, 15N- and dH-tracers.

Collaboration article https://doi.org/10.1016/j.redox.2022.102416, check publication list for additional publications.

Requires consultation and collaboration.

Sample types (minimum amount)
Biofluids (10µl)
Tissues
Cells (30000)
Organoids
Spheres
Extracellular vesicles

Select metabolites from 450+ list. Direct orders via iLAB or for further information contact fimm-metabolomics@helsinki.fi.

We are open to develop via collaboration new metabolomics analytical methods both for precision metabolomics and research purposes. Contact the head of the unit.

We are open to develop via collaboration new metabolomics analytical methods both for precision metabolomics and research purposes. Contact the head of the unit.

As an example, these compounds have been quantitated from customer samples.
Riboflavin
GABA
L-Cystine
Pyruvate
Myo-inositol
Cortisol
Biotin
3,3',5'-Triiodothyronine
Glutathione
GSSG
 

We offer via collaboration help for metabolomics/lipidomics data analysis.
Contact the head of the unit.

We are open for consulting patient and clinical metabolomics work, teaching, seminar and conference talks. 
Contact the head of the unit.

Multiomics workflows:

HiLIFE bioinformatics multi-omics data analysis service workflow will be launched 2025 ->

further information: anni.nieminen@helsinki.fi

 

HiLIFE single-cell omics service workflow will be launched 2025 ->

further information: anni.nieminen@helsinki.fi

 

General info

The compounds measured with the methods are listed under each method. If the sample amount is very low and/or the matrix is new the detectability of the compounds of interest must be checked with a test sample before running the actual samples.

Results are delivered in an excel file. Fluxomics and 450+ metabolites methods are relatively quantitative and the results are given as peak areas. In all other quantitative methods the results are given in µM or other suitable concentration unit.

The raw files are also available if required.

Leftover samples are discarded unless otherwise agreed.

Raw data and all processing files are stored for 2 years after reporting.