We design and optimize CRISPR/Cas9 single or double guide RNAs for genome editing projects in mammalian cell lines. The service can be requested together with gRNA validation or analytical services (see below for specification and prices) on CRISPR/Cas9 edited cell pools or clones.
FINGEEC provides following analytical services on CRISPR/Cas9 edited cell pools or monoclonal cells:
Mutation detection analysis identifies and measures genome editing efficiency by detecting indels (insertions or deletions). Our services include:
Clonal sequence analysis with sanger sequencing is used to identify mutation (mismatches, insertions or deletions of nucleotides) within the single or double guide RNAs target region in single cell clones. The genomic DNA must be extracted from single cell clones after CRISPR/Cas9 editing in desired cells for a clean sequencing result. Our service includes:
Validation services of genome editing (DNA) |
Fee (€) University of Helsinki |
Fee (€) Biocenter Finland |
---|---|---|
CRISPR guide RNA+PCR primer design and optimization | 93 | 116 |
CRISPR guide RNA+PCR primer design and optimization (Extra order) | 31 | 39 |
T7 nuclease mutation detection full service | 202 | 251 |
T7 nuclease mutation detection full service (Extra order) | 105 | 130 |
T7 nuclease mutation detection with PCR optimization (w/o gDNA isolation) | 188 | 233 |
T7 nuclease mutation detection with PCR optimization (w/o gDNA isolation) (Extra order) | 97 | 120 |
T7 nuclease mutation detection only (w/o gDNA isolation) | 136 | 168 |
T7 nuclease mutation detection only (w/o gDNA isolation) (Extra order) | 44 | 55 |
Clone sequence analysis with gDNA isolation | 185 | 230 |
Clone sequence analysis with gDNA isolation (Extra order) | 80 | 99 |
TOPO Clone analysis (single clone) | 305 | 389 |
TOPO Clone analysis (single clone) (Extra order) | 168 | 209 |
TOPO Clone analysis (polyclonal sample) | 402 | 500 |
TOPO Clone analysis (polyclonal sample) (Extra order) | 263 | 328 |
Finnish Genome Editing Center (FinGEEC) should be mentioned in the acknowledgment in research publications containing data from CRISPR analytics performed by FinGEEC. Below are the templates of sentences that can be used:
For analytical services including gRNA validation, T7 nuclease (SURVEYOR) assay, Sanger sequence analysis or TOPO assay:
"The xx analysis of xx (genes/KO/KIs) was performed by Finnish Genome Editing Center (FinGEEC), Research Programs Unit, Faculty of Medicine, University of Helsinki, supported by HiLIFE, University of Helsinki and Biocenter Finland."