Gateway cloning (cDNA)

Gateway® Cloning Technology is a cloning method developed by (originally Invitrogen) that allows for the transfer of DNA fragments between different cloning vectors while maintaining orientation and reading frame. It is a two-step, high-throughput system based on site-specific recombination (att sites) rather than traditional restriction enzyme digestion and ligation. 

Gateway cloning to your destination vector of choice.

We clone inserts from the ORFeome collection and other Gateway‑compatible PCR products or plasmids into a broad panel of destination vectors. Our vector portfolio spans bacterial and vertebrate expression systems as well as viral platforms, including lentiviral and AAV vectors. If you don’t see your preferred destination vector listed, we can consider adding it to our collection.

Need Gateway compatibility? We can generate it.

We can convert non‑Gateway templates into Gateway‑compatible PCR products, and then deliver your construct in the destination vector via a two‑step Gateway workflow.

The following control constructs can be cloned to all destination vectors.

• EmGFP with stop
• EmGFP without stop
• mCherry with stop
• mCherry without stop
• lacZ with stop
• lacZ without stop
• empty insert (the destination vectors with this insert contain no ccdb gene and can be grown in DH5alpha and stbl3 competent cells)

GBU also performs cloning reactions to customer-provided Gateway-compatible entry-constructs and PCR products. Please, deliver your samples to GBU in Biokeskus 1, room 3023. If you want to bring the samples yourself, please make an appointment to make sure we are in. 

You can also send your constructs by mail. .  

We collaborate with other cores in to provide services like virus production and creating over-expression cell lines. Together we can help you plan and execute the best experiment. You can contact the cores individually or reach us all by . 

If you use a clone from the GBU in your publication, please acknowledge us in the following way:

Clon­ing and mutagenesis:

“Constructs were generated by the Genome Biology Unit supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland"

OR­Feome clones:

“Name of the clone (ORFeome Library; Genome Biology Unit supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland)"

MGC clones:

“Name of the clone (MGC Library; Genome Biology Unit supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland)"