Pooled Libraries

Do you need your plasmid library expanded, or are you planning to create a custom overexpression library?
We can help design and carry out tailored pooled‑library projects to support your research.

Pooled Library Expansion from DNA Preparations

Service includes:

Pooled library delivered to us as DNA (e.g. )
Electroporation of the DNA into suitable E. coli cells
Collection of colonies ensuring at least 1000× library coverage
Preparation of pooled plasmid DNA using midipreps

Pooled DNA Preparation from Glycerol Stocks

We can prepare pooled DNA preparation directly from glycerol stocks. You can choose any clones from our collection:

: 16 934 human and 1 758 mouse cDNA clones
of shRNA clones
gRNA library

Or you can provide us:

Commercial pooled libraries as glycerol stocks, such as: Horizon

Pool Cloning of Synthetic Libraries

We can construct pooled libraries by cloning synthetic pooled constructs (e.g from ) into the expression vector of your choice.

For inquiries or project planning, please contact:

If your constructs are intended for viral delivery, we can forward the pooled plasmids directly to for virus production.

Acknowledging GBU in Publications

For publications containing pooled libraries expanded or cloned by the Genome Biology Unit (GBU), please include the following acknowledgement:

“Pooled library was expanded/cloned by the Genome Biology Unit supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland"