Site-directed mutagenesis

The mutagenesis service fee covers the site-directed mutagenesis reaction (two tries), as well as two miniprep DNA extractions and sequencing of two mutated plasmid clones. An additional fee is charged when competent cells other than standard Dh5alpha are needed (e.g. for lentiviral plasmids).

We use a Phusion HF polymerase with an error rate of 4,4 x 10-7, and while random mutations caused by the polymerase are rare, they are still possible. We recommend whole-plasmid sequencing to exclude replication errors outside the mutated region. 

We primarily do mutagenesis service only for the clones derived from our libraries. If you would like to have your own construct mutated, please contact us, and we will evaluate each service request case-by-case. The map and the full vector sequence are needed for the approved services. Because we can't guarantee the quality of customer's own plasmid we can't guarantee the success of the mutagenesis. We will make two trials but will need to charge them independent of the success. Failed mutagenesis is rare (<10%).

The mutated gene can then be cloned to expression vectors or viral vectors using or . 

If you use a clone from the GBU in your publication, please acknowledge us in the following way:

Clon­ing and mutagenesis:

“Constructs were generated by the Genome Biology Unit supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland"

OR­Feome clones:

“Name of the clone (ORFeome Library; Genome Biology Unit supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland)"

MGC clones:

“Name of the clone (MGC Library; Genome Biology Unit supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland)"