List of instruments available in DDCB - Faculty of Pharmacy unit. The unit is located in Biocenter 2, Viikki campus, University of Helsinki, Viikinkaari 5, E-tower, 4th floor. Instrument training requests and project requests are done through iLab system.
Varioskan LUX (Biocenter 2, room 4067)
- Multimode monochromator-based microplate reader for multiwell plates
- Three measurement technologies: Absorbance (UV-vis), Fluorescence intensity and Luminescence
- Spectral scanning for assay optimization
- Five measurement modes including end point, kinetic, spectra, kinetic spectra and multipoint
- Temperature control
- Equipped with reagent dispensing systems
- Automatic dynamic range selection, based on signal intensity of each well
- Automated instrument calibration and self-diagnostics for reliable performance
- Self-use is possible after short training/introduction
ChemiDoc MP (Biocenter 2, room 4067)
- Full-feature instrument for imaging and analyzing gels and western blots
- Can be used for multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs
- Chemidoc accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose Gels and various blots.
- This is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization.
- Self-use is possible after short training/introduction
NanoDrop One (Biocenter 2, room 4069)
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Microvolume UV-Vis Spectrophotometer
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Simple, rapid, accurate quantification and qualification of DNA, RNA, and protein
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Quantify DNA, RNA, and protein samples in seconds with only 1–2 µL your sample
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Measures also peptides at A205
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Self-use is possible after short training/introduction
Biomek i7 (Biocenter 2, room 4068)
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Automated pipetting workstation
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Liquid handling including pipetting, dilution, dispensing and integration
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Workstation can accomodate multiple plates at the same time
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96- and 384-pipetting heads
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Equipped with HEPA-filter (all procedures can be performed under sterile conditions)
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Washing station (tips can be washed and used again)
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Equipped with grippers to lid/unlid plates and move labware
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When using Biomek it is possible to purchase Biomek suitable tips and multi well plates as well
Mantis Liquid Handler (Biocenter 2, room 4080)
- Automated low-volume reagent dispensing system
- Accurate and precise dispensing up to 6 reagents in same set-up into 6-1536 well plates
- Dispensing volumes from 100nL at CV < 2%
- Located inside the BSL II class cabinet (Laminar 3) to enable aseptic workflows
- Miniaturize reactions, scale up throughput, save on reagents, and remove pipette tip cost
- The Mantis liquid dispenser dispenses volumes from 100 nL to 100s of mL allowing researchers to commonly run 2-5 µL reaction volumes in genomics and screening workflows
- To minimize dead volume, reagents plug directly into the microfluidic chip. Dead volumes are further reduced to just 6 μL by using pipette tips as reagent reservoirs, making the Mantis ideal for dispensing even the most precious reagents
Cytation 5 / BioSpa / MultiFlo FX (Biocenter 2, room 4068)
Each device can be used as separate unit or all linked together.
- Biospa: Automated incubator linked to the Cytation 5 imaging reader and MultiFlo FX Multimode Dispenser for fully automated workflows
- Temperature, CO2/O2 control, humidity monitoring, and lid handling ensure an ideal environment for cell cultures
- Multiflo FX: Automated multimode reagent dispenser for 6- to 1536-well microplates
Electroporator (Biocenter 2, room 4069)
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BTX Gemini X2 generator incorporates both square and exponential decay waves for electroporation of eukaryotic and prokaryotic cells
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Preset protocols – includes the most common eukaryotic and prokaryotic cell types
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User-defined protocols – unlimited ability to add and modify protocols
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Equipped with petri dish electrode for tissue electroporation, electrode kit for electroporation of adherent cells, and 96-well plate HTS electroporation module
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When using electroporator it is possible to purchase electroporation cuvettes and plates as well
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Self-use is possible after short training/introduction
Omnilog (Biocenter 2, room 4080)
- The Biolog Microbial ID System for incubation and identification of aerobic bacteria
- Enables testing and microbial identification of aerobic Gram-negative and Gram-positive bacteria in the same test panel. Gram stain and other pre-tests are no longer needed
- Possibility to incubate 50 microplates at same time
- Incubation temperature range 22° to 45° C
- Reading cycle time up to 25 trays with 50 Microplates in 15 mins
- Self-use is possible after short training/introduction
- To see more info from Biolog website: https://www.biolog.com/products/microbial-identification-microplates/ge…
LC-MS
- Xevo TQ-S tandem quadrupole mass spectrometer for the quantitative UPLC/MS applications
- Broad range of ion source available
- Qualitative and quantitaive analysis of small molecules and peptides
- Analytes are separated by UPLC and monitored by tandem mass spectrometry using multiple reaction monitoring (MRM)
- Both positive and negative ionizing compounds can be detected