Instruments - Pharmacy

List of instruments available in DDCB - Faculty of Pharmacy unit. The unit is located in Biocenter 2, Viikki campus, University of Helsinki, Viikinkaari 5, E-tower, 4th floor. Instrument training requests and project requests are done through iLab system.
Varioskan LUX (Biocenter 2, room 4067)
  • Multimode monochromator-based microplate reader for multiwell plates
  • Three measurement technologies: Absorbance (UV-vis), Fluorescence intensity and Luminescence
  • Spectral scanning for assay optimization
  • Five measurement modes including end point, kinetic, spectra, kinetic spectra and multipoint
  • Temperature control
  • Equipped with reagent dispensing systems
  • Automatic dynamic range selection, based on signal intensity of each well
  • Automated instrument calibration and self-diagnostics for reliable performance
  • Self-use is possible after short training/introduction
ChemiDoc MP (Biocenter 2, room 4058a)
  • Full-feature instrument for imaging and analyzing gels and western blots
  • Can be used for multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs
  • Chemidoc accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose Gels and various blots.
  • This is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization.
  • Self-use is possible after short training/introduction
NanoDrop One (Biocenter 2, room 4069)
  • Microvolume UV-Vis Spectrophotometer

  • Simple, rapid, accurate quantification and qualification of DNA, RNA, and protein

  • Quantify DNA, RNA, and protein samples in seconds with only 1–2 µL your sample

  • Measures also peptides at A205

  • Self-use is possible after short training/introduction

Biomek i7 (Biocenter 2, room 4068)
  • Automated pipetting workstation

  • Liquid handling including pipetting, dilution, dispensing and integration

  • Workstation can accomodate multiple plates at the same time

  • 96- and 384-pipetting heads

  • Equipped with HEPA-filter (all procedures can be performed under sterile conditions)

  • Washing station (tips can be washed and used again)

  • Equipped with grippers to lid/unlid plates and move labware

  • When using Biomek it is possible to purchase Biomek suitable tips and multi well plates as well

Mantis Liquid Handler (Biocenter 2, room 4080)
  • Automated low-volume reagent dispensing system
  • Accurate and precise dispensing up to 6 reagents in same set-up into 6-1536 well plates 
  • Dispensing volumes from 100nL at CV < 2%
  • Located inside the BSL II class cabinet (Laminar 3) to enable aseptic workflows
  • Miniaturize reactions, scale up throughput, save on reagents, and remove pipette tip cost
  • The Mantis liquid dispenser dispenses volumes from 100 nL to 100s of mL allowing researchers to commonly run 2-5 µL reaction volumes in genomics and screening workflows
  • To minimize dead volume, reagents plug directly into the microfluidic chip. Dead volumes are further reduced to just 6 μL by using pipette tips as reagent reservoirs, making the Mantis ideal for dispensing even the most precious reagents
Cytation 5 / BioSpa / MultiFlo FX (Biocenter 2, room 4068)

Each device can be used as separate unit or all linked together.

  • Cytation: Imaging multimode reader for automated imaging and/or conventional microplate detection
  • The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast
  • The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection
  • Temperature, CO2 control 
  • Linked to automated BioSpa incubator to enable kinetic imaging of up to 8 multiwell plates in parallel
  • When using Cytation it is possible to purchase suitable imaging plates as well

 

  • Biospa: Automated incubator linked to the Cytation 5 imaging reader and MultiFlo FX Multimode Dispenser for fully automated workflows 
  • Temperature, CO2/O2 control, humidity monitoring, and lid handling ensure an ideal environment for cell cultures

 

  • Multiflo FX: Automated multimode reagent dispenser for 6- to 1536-well microplates
Electroporator (Biocenter 2, room 4069)
  • BTX Gemini X2 generator incorporates both square and exponential decay waves for electroporation of eukaryotic and prokaryotic cells

  • Preset protocols – includes the most common eukaryotic and prokaryotic cell types

  • User-defined protocols – unlimited ability to add and modify protocols

  • Equipped with petri dish electrode for tissue electroporation, electrode kit for electroporation of adherent cells, and 96-well plate HTS electroporation module

  • When using electroporator it is possible to purchase electroporation cuvettes and plates as well

  • Self-use is possible after short training/introduction

Omnilog (Biocenter 2, room 4080)
  • The Biolog Microbial ID System for incubation and identification of aerobic bacteria
  • Enables testing and microbial identification of aerobic Gram-negative and Gram-positive bacteria in the same test panel. Gram stain and other pre-tests are no longer needed
  • Possibility to incubate 50 microplates at same time
  • Incubation temperature range 22° to 45° C
  • Reading cycle time up to 25 trays with 50 Microplates in 15 mins
  • Self-use is possible after short training/introduction
  • To see more info from Biolog website: 
LC-MS
  • Xevo TQ-S tandem quadrupole mass spectrometer for the quantitative UPLC/MS applications 
  • Broad range of ion source available 
  • Qualitative and quantitaive analysis of small molecules and peptides
  • Analytes are separated by UPLC and monitored by tandem mass spectrometry using multiple reaction monitoring (MRM)
  • Both positive and negative ionizing compounds can be detected
Maestro Pro MEA system (Biocenter 2, room 4067)

The Axion Biosystems Maestro Pro is a label-free, real-time live-cell analysis platform that combines microelectrode array (MEA) and impedance technologies. It enables monitoring of cellular function, including neuronal and cardiomyocyte electrophysiology, as well as cell adhesion, proliferation, morphology, and barrier integrity.