Instruments - Pharmacy

List of instruments available in DDCB - Faculty of Pharmacy unit. The unit is located in Biocenter 2, Viikki campus, University of Helsinki, Viikinkaari 5, E-tower, 4th floor. Instrument training requests and project requests are done through iLab system.
Varioskan LUX (Biocenter 2, room 4067)
  • Multimode monochromator-based microplate reader for multiwell plates
  • Three measurement technologies: Absorbance (UV-vis), Fluorescence intensity and Luminescence
  • Spectral scanning for assay optimization
  • Five measurement modes including end point, kinetic, spectra, kinetic spectra and multipoint
  • Temperature control
  • Equipped with reagent dispensing systems
  • Automatic dynamic range selection, based on signal intensity of each well
  • Automated instrument calibration and self-diagnostics for reliable performance
  • Self-use is possible after short training/introduction
ChemiDoc MP (Biocenter 2, room 4067)
  • Full-feature instrument for imaging and analyzing gels and western blots
  • Can be used for multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs
  • Chemidoc accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose Gels and various blots.
  • This is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization.
  • Self-use is possible after short training/introduction
NanoDrop One (Biocenter 2, room 4069)
  • Microvolume UV-Vis Spectrophotometer

  • Simple, rapid, accurate quantification and qualification of DNA, RNA, and protein

  • Quantify DNA, RNA, and protein samples in seconds with only 1–2 µL your sample

  • Measures also peptides at A205

  • Self-use is possible after short training/introduction

Biomek i7 (Biocenter 2, room 4068)
  • Automated pipetting workstation

  • Liquid handling including pipetting, dilution, dispensing and integration

  • Workstation can accomodate multiple plates at the same time

  • 96- and 384-pipetting heads

  • Equipped with HEPA-filter (all procedures can be performed under sterile conditions)

  • Washing station (tips can be washed and used again)

  • Equipped with grippers to lid/unlid plates and move labware

  • When using Biomek it is possible to purchase Biomek suitable tips and multi well plates as well

Mantis Liquid Handler (Biocenter 2, room 4080)
  • Automated low-volume reagent dispensing system
  • Accurate and precise dispensing up to 6 reagents in same set-up into 6-1536 well plates 
  • Dispensing volumes from 100nL at CV < 2%
  • Located inside the BSL II class cabinet (Laminar 3) to enable aseptic workflows
  • Miniaturize reactions, scale up throughput, save on reagents, and remove pipette tip cost
  • The Mantis liquid dispenser dispenses volumes from 100 nL to 100s of mL allowing researchers to commonly run 2-5 µL reaction volumes in genomics and screening workflows
  • To minimize dead volume, reagents plug directly into the microfluidic chip. Dead volumes are further reduced to just 6 μL by using pipette tips as reagent reservoirs, making the Mantis ideal for dispensing even the most precious reagents
Cytation 5 / BioSpa / MultiFlo FX (Biocenter 2, room 4068)

Each device can be used as separate unit or all linked together.

  • Cytation: Imaging multimode reader for automated imaging and/or conventional microplate detection
  • The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast
  • The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection
  • Temperature, CO2 control 
  • Linked to automated BioSpa incubator to enable kinetic imaging of up to 8 multiwell plates in parallel
  • When using Cytation it is possible to purchase suitable imaging plates as well

 

  • Biospa: Automated incubator linked to the Cytation 5 imaging reader and MultiFlo FX Multimode Dispenser for fully automated workflows 
  • Temperature, CO2/O2 control, humidity monitoring, and lid handling ensure an ideal environment for cell cultures

 

  • Multiflo FX: Automated multimode reagent dispenser for 6- to 1536-well microplates
Electroporator (Biocenter 2, room 4069)
  • BTX Gemini X2 generator incorporates both square and exponential decay waves for electroporation of eukaryotic and prokaryotic cells

  • Preset protocols – includes the most common eukaryotic and prokaryotic cell types

  • User-defined protocols – unlimited ability to add and modify protocols

  • Equipped with petri dish electrode for tissue electroporation, electrode kit for electroporation of adherent cells, and 96-well plate HTS electroporation module

  • When using electroporator it is possible to purchase electroporation cuvettes and plates as well

  • Self-use is possible after short training/introduction

Omnilog (Biocenter 2, room 4080)
  • The Biolog Microbial ID System for incubation and identification of aerobic bacteria
  • Enables testing and microbial identification of aerobic Gram-negative and Gram-positive bacteria in the same test panel. Gram stain and other pre-tests are no longer needed
  • Possibility to incubate 50 microplates at same time
  • Incubation temperature range 22° to 45° C
  • Reading cycle time up to 25 trays with 50 Microplates in 15 mins
  • Self-use is possible after short training/introduction
  • To see more info from Biolog website: https://www.biolog.com/products/microbial-identification-microplates/ge…
LC-MS
  • Xevo TQ-S tandem quadrupole mass spectrometer for the quantitative UPLC/MS applications 
  • Broad range of ion source available 
  • Qualitative and quantitaive analysis of small molecules and peptides
  • Analytes are separated by UPLC and monitored by tandem mass spectrometry using multiple reaction monitoring (MRM)
  • Both positive and negative ionizing compounds can be detected