Widefield Microscopes

Widefield microscopes at BIU.
EVOS

Inverted epifluorescence microscope with long working distance objectives for bright-field, phase contrast, and fluorescence microscopy. 

Mainly used for cells growing on plastic bottom dishes.

Fluorescence filters are integrated with LED light sources into 'light cubes'. The system has space for 4 such cubes or 3 + transmitted light.

Location: Room B504b

Tech­nical de­tails: BIU wiki

Reservations: Open IRIS

Zeiss Axio Im­ager 1

Motorized upright epifluorescence microscopes with transmitted light (phase and dark field) and a motorized stage.

Location: Room B502b

Tech­nical de­tails: BIU wiki

Reservations: Open IRIS

Zeiss Axio Im­ager 2 with ApoTome

Motorized upright epifluorescence microscopes with transmitted light (phase and dark field) and a motorized stage.

Zeiss ApoTome for optical sectioning with structured illumination.

Location: Room B502b

Tech­nical de­tails: BIU wiki

Reservations: Open IRIS

Leica Thunder Imager Model Organism

A Leica M 205 FCA stereo microscope with computational clearing, motorized stage, and stage top incubator for live imaging. The THUNDER Imager Model Organism allows fast and easy 3D exploration of whole organisms for developmental or molecular biology research. It is the optimal instrument for studying, for example, Drosophila, C. elegans, zebrafish, plants, and mice.

Location: Room A123b

Tech­nical de­tails: BIU wiki

Reservations: Open IRIS

Leica Thunder Imager 3D Cell Culture

Motorized Leica DMi8 inverted epifluorescence microscope with  motorized stage. 

  • Leica Thunder computational clearing for 3D imaging
  • Fast multichannel imaging
  • LAS X Navigator for multipoint imaging and large area stitching
  • OKOLab full environmental chamber for live cell imaging (10.11.2023)

Location: Room B115a2

Tech­nical de­tails: BIU wiki

Reservations: Open IRIS

Nikon Ec­lipse Ti-E for Op­to­gen­et­ics

Fully motorized inverted widefield microscope for fluorescence and transmitted light imaging with full environmental chamber,  Perfect Focus System for long time series and Lumencor Spectra X light engine

The NIS-Elements software modules allow the following imaging modes:

  • 2 channel ratio imaging
  • Fast imaging
  • High-Content Screening (HCS)

Location: Room B115a1

Tech­nical de­tails: BIU wiki

Reservations: Open IRIS

Nikon Eclipse Ti-E with Alveole Primo

Fully motorized inverted widefield microscope for fluorescence and transmitted light. Equipped with long working distance objectives, motorized stage, and Nikon JOBS module for automated high content image acquisition.

Alveole Primo micropatterning device for fast multi-protein photopatterning.

Location: Room B502b

Tech­nical de­tails: BIU wiki

Reservations: Open IRIS

Nikon Ec­lipse Ti-E with Andor Dragonfly Spinning Disk Confocal

Fully motorized inverted Nikon Eclipse Ti2 microscope with full environmental chamber and Perfect Focus System for long time series.

Andor Dragonfly 505 high speed spinning disc confocal.

  • 2 cameras
  • 5 laser lines (400, 488, 561, 642, and 730nm)
  • 2 confocal pinhole options (40 and 25µm)
  • TIRF
  • SRRF Stream super-resolution

Location: Room A509a2

Tech­nical de­tails: BIU wiki

Reservations: Open IRIS