Confocal and Multiphoton Microscopes

The spinning disc, laser scanning confocal, and multiphoton microscopes at the BIU. The multiphoton techniques include: two-photon excited fluorescence (TPEF), second harmonic generation (SHG), third harmonic generation (THG), coherent anti-Stokes Raman scattering (CARS).
Zeiss LSM 880

Inverted confocal microscope with GaAsP detector, AiryScan (Fast) detector, PicoQuant FLIM detector and full environmental chamber.

  • 2 PMT detectors (one with extra cooling for increased infrared sensitivity)
  • 1 GaAsP detector

Definite Focus hardware autofocus system

Scanning stage for multipoint or mosaic imaging

Location: Room B502b

Tech­nical de­tails:

Reservations:

Leica TCS SP8 CARS

Inverted confocal microscope with two HyD detectors, one PMT detector, resonant scanner, and full environmental chamber. The system is equipped with a tunable dual wavelength picosecond light source for multi-photon excitation (CARS, TPEF, SHG).

Location: Room B116a

Tech­nical de­tails:

Reservations:

Leica TCS SP8 X

Inverted confocal microscope with tunable white light laser source, two HyD detectors, two PMT detectors, and full environmental chamber.

  • HyVolution automated deconvolution
  • Matrix module for high-content screening

Location: Room A123b

Tech­nical de­tails:

Reservations:

Leica Stellaris 8 FALCON / DLS

Inverted confocal microscope with tunable white light laser source, 5 high end HyD detectors, and full environmental chamber. Equipped with additional resonant scanner for fast imaging with relatively low phototoxicity. Motorized stage for montage images.

  • DLS Digital Light Sheet ()
  • FALCON Fast Lifetime Contrast for Fluorescent Lifetime Imaging for FLIM experiments ()

Location: Room B501b

Tech­nical de­tails:

Reservations:

Andor Dragonfly Spinning Disc

Fully motorized inverted Nikon Eclipse Ti2 microscope with full environmental chamber and Perfect Focus System for long time series.

Andor Dragonfly 505 high speed spinning disc confocal.

  • 2 cameras
  • 5 laser lines (400, 488, 561, 642, and 730nm)
  • 2 confocal pinhole options (40 and 25µm)
  • TIRF
  • SRRF Stream super-resolution

Location: Room A509a2

Tech­nical de­tails:

Reservations: