The incidence of oral cancer is globally increasing and tongue squamous cell carcinoma (OTSCC) is the most common malignancy of the oral cavity. OTSCC represents the 8th most common cancer worldwide. Our research on OTSCC has two main focuses: First, to investigate the effects of tumor microenvironment (TME) components, such as tumor associated fibroblasts, macrophages, inflammatory cells and matrix molecules including extracellular vesicles in the progression and chemo-radiation therapies on OTSCC. Our research group has developed human myoma tumor tissue based models (myoma discs and myogel) which already are widely used for cancer invasion studies. We are developing the model further for various applications. Including cancer invasion and drug testing. Second, to identify the clinically most useful prognostic markers for improving early stage OTSCC diagnostics.