The Digital Geography Lab explores the use of novel big (and open) data sources and cutting-edge methods in supporting sustainable spatial planning and decision-making. Our main focus is on two intertwined areas of research: human mobility/accessibility and biodiversity conservation. We take advantage of new data sources, such as social media content, mobile phone call detail records, and public transport timetables, combined with remotely-sensed and register data, which we use for carrying out advanced spatial analyses of accessibility and mobility patterns, conservation opportunities and threats, and tracking illegal wildlife trade trafficking. Our research is conducted at various spatial scales in Finland, Estonia, South Africa and Uruguay, extending across continental and global scales. The Digital Geography Lab is also a forerunner in open science and actively shares its research tools and data with different stakeholders both within and outside academia.