TRC shRNA collection

The clones are available as glycerol stocks, plates, DNA minipreps or midipreps ().

If you need viral particles produced from the DNA preps, we can forward the DNA directly to the for lentivirus production. Please contact them directly to arrange for the virus production.

Clones are distributed to Biocenter-Finland affiliated groups only.

Our collection comprises 81 534 human shRNAs and 77 994 mouse shRNAs. There are usually five different constructs that target the same gene. The constructs are in pLKO.1 lentiviral vector with puromycin selection. Carbenicillin selection is used for the growth and maintenance of the vector in bacterial cells. See the vector map and sequence . 

More detailed information on the constructs can be found at the .

If our library does not cover your gene of interest, we can clone a custom shRNA into pLKO.1 vector or into inducible Tet-pLKO-puro vector. See for details.

Negative controls in our stock: 
SCH001: 
SHC002: 
SHC016:
Positive knockdown control:
shRNA targeting eGFP in pLKO.1 (request detailed information)
Positive control:
SHC003: 

To order clones or DNA,  fill the order form and send it to .

The delivery time is approximately one week.

We always recommend to sequence the clones before the experiments. The libraries are vendor-verified but unfortunately do contain some errors. You can find the sequencing primers suitable for your clone in the info sheet we send with your order. We offer a service to verify the clones by Sanger sequencing (approximately two days) or restriction analysis (one day). Please indicate in the form if you would like to purchase also the verification service and which method.

Please indicate in the form if you would like to submit the DNA directly to the for virus production. We will provide BVC with enough DNA for the desired product. Please contact for more information and orders. 

DNA preps are delivered by mail. Glycerol stocks are shipped with UPS or FedEx on dry ice on the customer's expense (customer's account number required for FedEx). For plates the delivery options are pick-up and UH internal mail. 

Usage of clones and acknowledgements

Please indicate GBU contribution in your publications (materials and methods section/acknowledgements) e.g. in the following way:

For TRC1 shRNA clones:
These clones (xx, yy) were from Sigma Aldrich MISSION shRNA library, distributed by Genome Biology Unit core facility supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland.

Please note that these reagents are solely for research purposes. See MTA (Material Transfer Agreement) at the .