CRISPR gRNA Collection

GBU offers pre-made CRISPR knockout plasmids from the Sanger gRNA library for lentiviral gene disruption. These clones are available to research groups within the Biocenter Finland network.
CRISPR gRNA Collection

The Sanger CRISPR gRNA library (Sigma-Aldrich) covers 40,803 mouse and 34,334 human gRNA constructs, usually two guides per gene.  All constructs are cloned into the LV04 lentiviral vector that includes pyromycin selection cassette and a blue fluorescent protein (BFP) tag for positive-cell visualization. 

You can search for your gene of interest using the list provided below.
The full are available at the distributor´s website.
Additional clone information can be obtained by searching for each specific gene at the website.

If suitable guides are not available, or if you need the guide cloned into another vector backbone, we can help design and clone custom gRNA constructs. See for details.

Controls

Available negative controls in LV04 vector:

  • Universal Non‑Target Control () that does not target known human, mouse, or rat genes.
  • Universal Non‑Target Control () that does not target known human, mouse, or rat genes.
Ordering, Delivery, and Prices

Please fill in the order form and send it to .

Please indicate in the form if you would like to submit the DNA directly to the for virus production. We will provide BVC with enough DNA for the desired product. Please contact for more information and orders.

Available delivery formats:

  • Plates
  • DNA mini/midipreps
  • Glycerol stocks

Delivery options:

  • Local pickup at Biocenter 1, Viikki
  • Internal mail (University of Helsinki)
  • External mail for DNA
  • UPS shipment on dry ice for glycerol stocks (shipping fees added to invoice)

Typical delivery time: within one week.

If your constructs are intended for viral delivery, we can forward them directly to HelVi‑BVC for virus production.
Clone Verification

Although the libraries are vendor‑verified, occasional errors may occur.
We strongly recommend sequencing all clones.

You will receive an info sheet with your delivery that lists the recommended Sanger sequencing primer for shRNA clones.

We also offer verification as a service, which can include:

  • Restriction analysis
  • Sanger sequencing
  • Whole‑plasmid sequencing

For sequencing, alignment of your construct sequence to the original library reference includes the service, as well as a full sequencing report.

Faulty clones will not be charged.
Acknowledging GBU in Publications

If you use a clone from the GBU in your publication, please acknowledge us in the following way:

For CRISPR clones:
These clones (xx, yy) were from Sanger gRNA library, distributed by Genome Biology Unit core facility supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland.

Please note that these reagents are solely for research purposes. See MTA (Material Transfer Agreement) at the .