The constructs are in LV04 lentiviral vector that includes pyromycin selection cassette and a blue fluorescent protein (BFP) tag for positive-cell visualization. Ampicillin is used for selection in bacteria.
You can search for your target gene in our library from the xlsx-file below. If you don't find suitable guides, or if the library vector is not what you need, we can help you design custom clones and clone them into a vector of your choice, see
Available negative controls:
The full vector map and sequence can be found
You can find more information on the clones on the distributor website:
The clones are available as glycerol stocks, plates, DNA minipreps or midipreps. Clones are distributed to Biocenter-Finland affiliated groups only.
To order clones or DNA, fill the order form and send it to
The delivery time is approximately one week.
We always recommend to sequence the clones before the experiments. The libraries are vendor-verified but unfortunately do contain some errors. You can find the sequencing primers suitable for your clone in the info sheet we send with your order. We offer a service to verify the clones by Sanger sequencing (approximately two days) or restriction analysis (one day). Please indicate in the form if you would like to purchase also the verification service and which method.
If you need virus particles produced from the DNA preps, we can forward the DNA directly to the
DNA preps are delivered by mail. Glycerol stocks are shipped with UPS or FedEx on dry ice on the customer's expense (customer's account number required for FedEx). For plates the delivery options are pick-up and UH internal mail.
Usage of clones and acknowledgements
Please indicate GBU contribution in your publications (materials and methods section/acknowledgements) e.g. in the following way:
For CRISPR clones:
These clones (xx, yy) were from Sanger gRNA library, distributed by Genome Biology Unit core facility supported by HiLIFE, Faculty of Biological and Environmental Sciences, University of Helsinki, and Biocenter Finland.
Please note that these reagents are solely for research purposes. See MTA (Material Transfer Agreement) at the