Sanger sequencing

FIMM Technology Centre's SeqLab provides DNA sequencing services for the scientific community nationally and internationally. In addition to DNA sequencing, small to medium scale fragment analysis service is also available. Sequencing system used is Applied Biosystems ABI3730XL DNA Analyzer. As a starting material we use purified PCR products mixed with sequencing primer or ready made sequencing/fragment analysis reactions. Maximum read length (QV>20) for high quality sequencing product is around 600bp.

For more detailed instructions please contact fimm-seqlab(at)helsinki.fi

 

 

  • Optional BigDye v.3.1 sequencing reaction for customer's template-primer cocktails.
  • Sequencing reaction purification: dye terminator removal with Optima DTR 96-Well filter plates.
  • Electrophoresis with ABI3730xl DNA Analyzer.
  • Basecalling with Sequencing Analysis 5.2.
  • Results delivered via e-mail (.ab1 file format).