In addition to DNA sequencing, small to medium scale fragment analysis service is also available. Sequencing system used is Applied Biosystems ABI3730XL DNA Analyzer. As a starting material we use purified PCR products mixed with sequencing primer or ready made sequencing/fragment analysis reactions. Maximum read length (QV>20) for high quality sequencing product is around 600bp.
SeqLab uses HiLife iLab online system for managing orders and invoices. In iLab you will find the sample information form along with up-to-date pricing for available services. We are using iLab as the primary communication channel regarding possible breaks in sample processing due to instrument maintenance, staff holidays etc.
Below are instructions on how to register to iLab and create a service request for Sanger and fragment analysis services.
BigDye v.3.1 sequencing reaction for customer provided template-primer mix
Sequencing reaction purification: dye terminator removal with Optima DTR 96-Well filter plates
Electrophoresis with ABI3730xl DNA Analyzer
Basecalling with Sequencing Analysis 7
Results delivered via e-mail (.ab1 file format
Sequencing reaction purification: dye terminator removal with Optima DTR 96-Well filter plates
Electrophoresis with ABI3730xl DNA Analyzer
Basecalling with Sequencing Analysis 7
Results delivered via e-mail (.ab1 file format)
Electrophoresis with ABI3730xl DNA Analyzer
Initial electrophoresis run quality assessment
Results delivered via e-mail (.ab1 file format)