On this page you can get acquainted with basic flow cytometry and find some help for planning your experiments.
Flow Cytometry Protocols Editors: Hawley, Teresa S., Hawley, Robert G. (Eds.)
Modern Concepts of Flow Cytometry Barbara Roth (editor)
How to prepare my sample?
Sample needs to be in a single cell suspension. Preferably in PBS or low seerum. The ideal concentration, which would work for all cell types, is impossible to define. The starting point could be 1-10 million cells / ml. Usually, the easiest way is to start with too high concentration and then dilute the suspension when needed (in this case, don’t forget to bring appropriate buffer with you).
Always filter samples before sort and remember to have buffer for the sort collection.
For set up, it is always advisable to have an unstained control sample and multicolor experiments will need all separately stained controls as well