Location: Room 1411.2
High Pressure Freezing (HPF) is the way to localise or characterise organelles, subcellular components and gene products in EM without the disadvantages of classical fixation methods:
Location: Room 1411.2
The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins.
The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications.
Location: Room 1411.2
For the preparation of vitrified fluid samples or extremely thin samples for cryo-TEM, including biological suspensions and industrial emulsions in both, aqueous and inorganic solvents.
The Leica EM GP plunge freezes samples into liquid ethane after removing excess fluid by automatic blotting. This is the bare grid technique. Prior to freezing the sample is maintained in a temperature and humidity controlled environmental chamber, adjustable between +4°C and +60°C and room humidity to 99%.
The EM GP can be used to plunge freeze samples not only on EM grids, but also sapphire discs and samples in freeze fracture planchettes.
Please contact CryoEM personnel for details of use.
Low temperature sectioning system for biological and industrial samples at temperatures as low as -185°C. The Leica EM FCS cryo-chamber is installed onto the Ultracut UCT. Sections can be made on either glass or diamond knives.
Location: Room 1412
(Haug Gmbh & Co. Switzerland)
Diatome cryotrim & cryoP diamond knives (Diatome, Switzerland)