Services routinely provided by FuGU include targeted DNA sequencing (exome, ready-made and custom gene panels) using a number of different library preparations systems and Miseq or NextSeq500 instrumentation. Sequencing read length ranges from 36 to over 300 bases depending on the instrument. The service includes sample QC, library preparation, sequencing, data pre-processing and analysis if requested by the customer.
FuGU provides a wide variety of RNA-seq services (e.g. whole transcriptome, 3' counting, smallRNA) using Illumina sequencing instrumentation (MiSeq, NextSeq500, access to NovaSeq). Gene expression profiling using Agilent microarray systems is also possible. Transcriptome analysis includes nucleic acid QC, sample labelling, hybridization or sequencing and pre-processing of the data. Downstream data analysis is offered using a number of different options depending on the application.
NanoString nCounter assays can be applied for targeted mRNA, miRNA and protein expression, SNV, CNV and gene fusion analyses. The technology is based on direct hybridization of target-specific color-coded probes and therefore, no cDNA synthesis or amplification of RNA/DNA is required. Starting material can originate from FFPE samples, tissue, cells, or plasma.
We provide genotyping services by MiSeq FGx™ Forensic Genomics System which is designed to detect more than 200 DNA markers in a single reaction. The markers have been designed for identity testing, estimation of ancestry, and population genetic analysis. Thus, the system is versatile and can be applied to track the mix-up of samples, such as cell lines or biopsies and formalin-fixed samples from pathology or service laboratories. The ForenSeq system is based on the detection of short tandem repeats and single-nucleotide polymorphisms specific for sex chromosomes, as well as e.g. hair and eye colour. The minimum amount of starting material is as low as 100 pg of DNA.
FuGU has low and high-throughput options for RT-qPCR using Roche LightCycler 4800 and Fluidigm Biomark HD and NGS to e.g. validate the findings from whole transcriptome analyses. Roche LightCycler is cost-effective in small projects (up to 12 samples and 12 genes), whereas Fluidigm Biomark HD, where reactions are carried out in nanoliter volumes, becomes the cheapest and fastest option when the number of samples and genes is high.
This service has been set-up in 2015 and it is based on detecting specific protein expression by antibodies that are linked with oligos. Each protein is detected by two antibodies, which are linked with complementary oligos. Binding of the oligos can be detected and protein expression from up to 90 proteins from 92 samples is quantified using qPCR.
The QC analysis from thousands of different samples is carried out in FuGU each year. We have different instruments (Qubit, NanoDrop Agilent 2100 Bioanalyzer and TapeStation) for measuring the concentration, purity and size of nucleic acids depending on the application. We also provide RNA or DNA extraction from blood, cells or fresh frozen tissue sample. RNA and DNA can also be extracted from the same sample if requested by the customer.
We provide computational analyses for both next-generation sequencing and microarray data. The analysis services are categorized into basic and advanced data analysis according to the scope and objectives of the study.