Biomedicum Functional Genomics Unit

Biomedicum Functional Genomics Unit (FuGU) provides a wide range of Illumina next-generation sequencing (NGS), NanoString, microarray and qRT-PCR services nationally and internationally. Our focus is in transcriptomics services as well as services for targeted genomics covering exome sequencing and focused gene panels. Our services cover consultation in experiment design, sample QC, sample processing for NGS or microarrays, sequencing using Illumina technology (NextSeq 500 and MiSeq FGx) and computational data analysis.

In case you are interested in FuGU/Libraries services, such as Biomedicum Virus Core (BVC), shRNA/guide RNAs or viral particles, please click here: https://www.helsinki.fi/en/infrastructures/genome-editing-function-and-s...

Contact persons:

For general enquires: fugu-support@helsinki.fi + 358 2 941 25188

Director Outi Monni: outi.monni@helsinki.fi +358 40 7639302

Lab Coordinator Martyn James: martyn.james@helsinki.fi +358 50 4486426

Location:

Biomedicum Functional Genomics Unit, Rooms C502b (Office)/C504b (lab), 5 krs, Biomedicum I building, Haartmaninkatu 8, University of Helsinki, 00290 Helsinki, Finland

Ordering form:

https://fugu.innolims.fi/fugu/public/julkinen/w/fugu/AnalyysiTilaus.aspx?kustnro=GenProf&lomake=BC&kieli=ENG

Services:

Illumina-based Next-Generation sequencing including:

  • RNAseq (whole transciptome, coding transcriptome, 3'RNAseq, miRNAs and other small RNAs, gene panels)
  • DNAseq (exome-seq, targeted custom or ready-made gene panels, WGS small genomes)
  • Methylseq (bisulfite sequencing)
  • ForenSeq (200 STR and SNP markers for identity testing, estimation of ancestry, population genetics, tracking sample mix-ups)

NanoString nCounter SPRINT

  • Gene Expression panels, Vantage 3D RNA and protein panels, miRNA panels

Agilent gene expression microarrays and copy number analysis (arrayCGH)

Fluidigm Biomark HD-based assays including:

  • Olink Proteomics’ Biomarker Panels
  • High-throughput qPCR

Microarray slide scanning

For reservation: https://www.brownbearsw.com/cal/scanner_bbc

Nucleic acid QC analyses

For reservation: https://www.brownbearsw.com/cal/bioanalyzer1

Bioinformatics data analysis

Instruments:

  • Illumina NextSeq500 and MiSeqFGx
  • Agilent SureScan Dx Microarray Scanner and Hybridization Oven
  • Fluidigm Biomark HD
  • NanoString nCounter SPRINT
  • Agilent 2100 Bioanalyzer
  • Agilent 4200 Tapestation
  • Qubit Fluorometer
  • Victor Nivo multiplate reader
  • Thermo Nanodrop
  • Covaris S2 Sonicator
  • Three PCR machines (Bio-Rad T100, S1000 and Finnzyme DNA Engine)

User Fees:

Pricing is calculated according to the guidelines provided by the University of Helsinki. Pricing has to be approved by the Director of the University of Helsinki’s Research Programs Unit. The research infrastructure is supported by University of Helsinki (HiLIFE) and Biocenter Finland.

Example prices for University of Helsinki customers (VAT 0%). Please contact fugu-support@helsinki.fi to get exact up-to-date pricing and full list of available services.

Illumina sequencing:

  • DNAseq library preps: Prices start from 63€/sample
  • RNAseq library preps:
    • 3'RNAseq/Bulkseq from 25€/sample
    • Coding transcriptome from 145€/sample
    • Whole transcriptome from 213€/sample
  • Exome-seq library prep and capture: Prices start from 300€/sample
  • MiSeq V3 (2 x 300 bp) approx. 2000€/run
  • NextSeq 500 High Output (1 x 75 bp) 400 M SE reads -  1945€/run
  • NextSeq 500 High Output (2 x 75 bp) 400 M PE reads -  3476€/run

Fluidigm Biomark HD

  • Olink Proteomics’ Biomarker Panels - approx 7500€/panel
  • 96.96 Gene Expression array (9216 data points) - 2700€/array