Clones and Cloning

GBU's reagent collection contains a library of open reading frames (ORFeome collaboration) consisting of about 18 600 fully verified clones, and a collection of full-length cDNAs (MGC collection) consisting of about 29 000 clones. The ORF library clones are available in Gateway-based entry vectors and we provide cloning service into various Gateway destination vectors for Biocenter Finland research groups.

Libraries

orfeome

ORFeome Collaboration (full ORFs in Gateway entry vectors):

human clones      16934

mouse clones       1758

MGC

MGC collection (cDNA clones in various vector backbones):

human clones      20 945

mouse clones       8290

Clones in the libraries are glycerol stocks, provided as streaks on bacterial plates.

We also have control vectors with EmGFP or mCherry as the insert in various Gateway expression vectors. Enquire for more information on the control vectors. 

Cloning service

We do cloning from the ORFeome collection, as well as from your own Gateway-compatible PCR products and plasmids to a number of destination vectors. Additionally we provide help in designing primers to create Gateway-compatible PCR products. The cloned products are delivered as transfection grade minipreps and glycerol stocks. Minipreps can be delivered by mail if necessary.

GBU gateway cloning

Feel free to suggest updates to our collection of destination vectors. 

Clone requests

Look up your gene of interest in the libraries and copy paste the data to the following request form. If cloning to another vector is needed, please specify the vector in the form. Email the form to bch-gbu (a) helsinki.fi.

Sample delivery

GBU also performs cloning reactions to customer-provided Gateway-compatible entry-constructs and PCR products. Please, deliver your samples to GBU sample fridge (Biomedicum Helsinki 1, 5th floor, room B505b). If you send samples by mail please use the address below:

Genome Biology Unit (GBU)
Haartmaninkatu 8
Biomedicum Helsinki 1
00014 University of Helsinki
5th floor, room B505b
P.O.Box. 63

Prices

The clones from the libraries are provided only to Biocenter Finland research groups. The clones are provided free of charge but a packaging fee of 20€ is charged for delivered clones. Cloning reactions are 40€ / cloning. 

Examples:
Cloning of an ORFeome clone to an expression vector: processing fee of the clone: 20€, LR cloning to an expression vector: 40€, total 60€. 

Cloning of your own PCR product to expression vector: PCR primer design free of charge, BP cloning to entry-vector: 40€, LR cloning to an expression vector: 40€, total 80€.

Control vectors: 20€ / miniprep. 
 

Citation

If you use a clone from the GBU in your publication, please acknowledge us in the following way:

Clon­ing:

“Constructs were generated by the Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland"

OR­Feome clones:

“Name of the clone (ORFeome Library; Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland)"

MGC clones:

“Name of the clone (MGC Library; Genome Biology Unit supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland)"