General fearfulness, sensitivity to noise as well as hyperactivity and impulsiveness are the most common behavioural problems in dogs. At their worst, they can have a very negative impact on the wellbeing of the dog and owner alike.
“Behaviour and behavioural disorders often develop as a combination of hereditary and environmental factors, which makes studying them challenging. Metabolomics, or the study of the metabolism, provides us with new clues on the biological issues underpinning behavioural disorders while promoting genetic research. At the moment, metabolomics research in dogs is rare, and the purpose of this pilot study was to examine new approaches and attain information on any metabolic abnormalities associated with hyperactivity in dogs," explains Professor Lohi.
Abnormal metabolic blood test results in hyperactive dogs
Determining the blood metabolites in hyperactive and normally behaved German Shepherds revealed a significant link between hyperactivity and lower blood phospholipid levels.
“We knew to expect this discovery from research on the human side, as several studies have recorded lower blood lipid and fatty acid levels in ADHD patients than in control groups. However, the causal relationship is not clear and requires further studies, particularly ones with more extensive research data. Our discovery supports the existing belief that human and canine diseases are similar, which suggests dogs can serve as excellent models for human illnesses,” states doctoral student Jenni Puurunen.
“It is significant that the dog’s age, sex or fasting had little impact on the link between behaviour and metabolites. We also controlled for dietary changes by feeding all dogs the same food for two weeks before testing,” explains Puurunen.
Intestinal health can impact canine behaviour
One of the most interesting discoveries in the study was the negative correlation between hyperactive behaviour and the levels of the metabolites of tryptophan, a vital amino acid. This metabolite is only produced when intestinal bacteria process the tryptophan received in food. The discovery suggests differences in the gut bacteria of hyperactive and normally behaved dogs, which is very significant in light of the discovery made a few years ago about the connection between the brain and the intestines.
“We know that the composition of the gut microbiota significantly influences the creation of neurotransmitters, for example, those which regulate mood and behaviour. The effect also works vice-versa, so that a stress reaction in the brain can have an adverse effect on the gut microbiota. Consequently, we cannot tell whether our discovery is the cause of canine hyperactivity or its consequence,” Puurunen says.
A globally unique metabolomics project is underway
Earlier this year, Lohi’s research group released an article on a study of the metabolomics of fearful dogs, which revealed differences between the blood counts of fearful and fearless dogs. However, more extensive research is required to confirm these pilot-stage findings. The research group has launched an extensive collection of samples to test new metabolomics technology together with the company Genoscoper. If successful, the new system could become a significant tool to speed up genetic research, particularly as it relates to behavioural studies.
The study is part of a more extensive canine behaviour project underway at the research group. The project seeks to determine the environmental and hereditary factors as well as metabolic changes relating to behaviour and behavioural disorders, and map their similarities with corresponding illnesses in humans.
Professor Hannes Lohi’s research group works in the University of Helsinki’s Faculty of Veterinary Medicine and Faculty of Medicine as well as at the Folkhälsan Research Centre. Professor Lohi’s research has received support from the University of Helsinki, the Academy of Finland, Biocentrum Helsinki, the Jane and Aatos Erkko Foundation, the Canine Health Research Fund and the Folkhälsan Research Centre.
Puurunen J, Sulkama S, Tiira K, Araujo C, Lehtonen M, Hanhineva K and Lohi H. A non-targeted metabolite profiling pilot study suggests that tryptophan and lipid metabolisms are linked with ADHD-like behaviours in dogs. Behav Brain Funct, 12:27, 2016. DOI: 10.1186/s12993-016-0112-1
Puurunen J, Tiira K, Lehotinen M, Hanhineva K and Lohi H. Non-targeted metabolite profiling reveals changes in oxidative stress, tryptophan and lipid metabolisms in fearful dogs. Behav Brain Funct 2016, 12(1):7-016-0091-2