Matrix profiling (100-200 metabolites) with Identification and relative quantitation (peak area) of metabolites using HRMS and inhouse library for RTs of 470 run standards and m/z.
The exact number of detected metabolites is dependent on sample matrix and sample amount.
Throughput approximately 150 samples per week. Service available in iLAB.
Collaboration article DOI:
Sample types (minimum amount)
Biofluids
Serum (15-110 µl)
Plasma (15-110 µl)
CSF (15-110 µl)
Sweat (10 µl)
Tears (10 µl)
Whole blood (40µl)
Tissues (0.5-20 mg, suggested 20mg)
Mouse heart (0.5 mg-)
Spleen (20 mg)
Brain (5-10mg)
Muscle (2-20mg)
Cecum
Gut
Skin (2-5mg)
Mucus
Others
Feces 30mg
Cells (10 000-2x10e6)
Organoids
Spheres
Bacteria, Yeast
Extracellular vesicles (10 µl)
Cell media (10-110µl)
Isotope enrichment analysis and metabolic flux analysis of central carbon metabolism, glucose, NADolome, glutamine and other pathways using 13C-, 15N- and dH-tracers.
Suitability (matrix protocol existing, tracer analytics existing, experimental set up, adequate funding) of the workflow is evaluated before accepting the service request.
Requires consultation and evaluation of the project and analysis suitability, in many projects requires intellectual input from core unit.
Sample types (minimum amount)
Biofluids (10µl)
Tissues
Cells (30000)
Organoids
Spheres
Extracellular vesicles
MS imaging of small molecules
New projects are evaluated for feasibility including funding, sample type, metabolites of interest.
Contact:
Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.
Compounds
NOTE: Folate samples must be stored in amber coloured vials.
Sample types (minimum amount)
Whole blood (60 µl)
Tissues (20 mg)
Cells (1x10e6)
CSF (15 µl)
Identification and absolute quantitation with internal standards using high resolution orbitrap mass spectrometer.
Throughput approximately 150 samples per week. Service available from iLAB.
Compounds.
Reduced prices are available if analyzing only NAD and NADH or NAD, NADH, NADP and NADPH.
Sample types (minimum amount)
Whole blood (60 µl)
Identification and absolute quantitation with internal standards using high resolution orbitrap mass spectrometer.
Throughput approximately 150 samples per week.
Compounds.
Sample types (minimum amount)
Biofluids
Serum (110 µl)
Plasma
Whole blood
Tissues
Cecum (20 mg)
Others
Feces (10 mg)
Cells
Organoids
Spheres
Bacteria
Extracellular vesicles
Quantitative LC-MS concentration measurement using orbitrap
Compounds.
Sample types (minimum amount)
Biofluids (serum, plasma) (110 µl)
Tissues (15 mg) (under development)
Cells (1.5x10e6) (under development)
Analysis of thousand lipid molecular species in a single method using the Lipidyzer platform.
More details
Sample types (minimum amount)
Biofluid (25 µl)
Tissues (15 mg)
Cells (1x10e6), Nanovesicles (25ul)
For further information iLAB or contact core
Select metabolites from 450+ list. Direct orders via iLAB or for further information contact
We are open to develop via collaboration new metabolomics analytical methods both for precision metabolomics and research purposes. Contact the head of the unit.
We are open to develop via collaboration new metabolomics analytical methods both for precision metabolomics and research purposes. Contact the head of the unit.
As an example, these compounds have been quantitated from customer samples.
Data analysis xx€/hour via iLAB
1. Missing Value imputationData normalisation (log transformation and auto scaling)
2. Univariate analysis methods: Fold Change Analysis, T-tests, Volcano Plot, One-way ANOVA and post-hoc analysis
3. Multivariate analysis methods: Principal Component Analysis (PCA), Partial Least Squares - Discriminant Analysis (PLS-DA)
4. Clustering Analysis: Hierarchical Clustering, Heatmap
We offer also via collaboration help for metabolomics data analysis.
Contact the director of the unit.
Opened spring 2025
Quantitative LC-MS concentration measurement using internal standard with triple quadrupole or high-resolution orbitrap mass spectrometer.
Compounds.
Sample types (minimum amount)
Biofluids (110 µl)
Quantitative LC-MS concentration measurement using internal standard with MRM
Suitable for tissues, cells, and biofluids
Sample types (minimum amount)
Biofluids (110 µl)
Tissues (15 mg)
Cells (1x10e6)
We are open for consulting patient and clinical metabolomics work, teaching, seminar and conference talks.
Contact the head of the unit.
Multiomics data workflows:
HiLIFE bioinformatics multi-omics (Metabolomics, genomics, trasncriptomics, proteomics) data analysis service workflow is under development during 2025-2026
further information:
Multiomics analytical workflows
From same sample: Metabolomics + Proteomics
From separate samples: bulk Metabolomics + bulk RNA seq + bulk proteomics
further information:
The compounds measured with the methods are listed under each method. If the sample amount is very low and/or the matrix is new the detectability of the compounds of interest must be checked with a test sample before running the actual samples.
Results are delivered in an excel file. Fluxomics and 450+ metabolites methods are relatively quantitative and the results are given as peak areas. In all other quantitative methods the results are given in µM or other suitable concentration unit.
The raw files are also available if required.
Leftover samples are discarded unless otherwise agreed.
Raw data and all processing files are stored for 3 months after reporting.