Vizcaíno-Castillo A, Kotila T, Kogan K, Yanase R, Como J, Antenucci L, Michelot A, Sunter JD, Lappalainen P. Leishmania profilin interacts with actin through an unusual structural mechanism to control cytoskeletal dynamics in parasites. J Biol Chem. 2024 Feb 9;300(3):105740.
Ashwini Kedari, Rommel Iheozor-Ejiofor, Lev Levanov, Kalle Saksela, Olli Vapalahti, Ilona Rissanen. Structural insight into rabies virus neutralization revealed by an engineered antibody scaffold. bioRxiv 2023.10.30.564668; doi: https://doi.org/10.1101/2023.10.30.564668.
Eronen A, Nieger M, Kajander TA, Repo T. Stereospecific Synthesis of Cyclohexenone Acids by [3,3]-Sigmatropic Rearrangement Route. J Org Chem. 2023 Sep 15;88(18):12914-12923. doi: 10.1021/acs.joc.3c00757. Epub 2023 Sep 1. PMID: 37656942.
Liu Y, De Castro Ribeiro O, Haapanen O, Craven GB, Sharma V, Muench SP, Goldman A. Unexpected structures formed by the kinase RET C634R mutant extracellular domain suggest potential oncogenic mechanisms in MEN2A. J Biol Chem. 2022 Oct;298(10):102380. doi: 10.1016/j.jbc.2022.102380. Epub 2022 Aug 17. PMID: 35985422; PMCID: PMC9490035.
Kogan K, Haapasalo K, Kotila T, Moore R, Lappalainen P, Goldman A, Meri T. Mechanism of Borrelia immune evasion by FhbA-related proteins. PLoS Pathog. 2022 Mar 18;18(3):e1010338. doi: 10.1371/journal.ppat.1010338. PMID: 35303742; PMCID: PMC8967061.
Beyer HM, Iwaï H. Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins. Front Mol Biosci. 2022 Mar 16;9:855511. doi: 10.3389/fmolb.2022.855511. PMID: 35372505; PMCID: PMC8966425.
Kim J, Kim S, Kim H, Hwang IW, Bae S, Karki S, Kim D, Ogelman R, Bang G, Kim JY, Kajander T, Um JW, Oh WC, Ko J.MDGA1 negatively regulates amyloid precursor protein-mediated synapse inhibition in the hippocampus. Proc Natl Acad Sci U S A. 2022 Jan 25;119(4):e2115326119.
Karki S, Kajander T. Crystallization and low-resolution structure solution of the SALM3-PTPσ synaptic adhesion complex. Acta Crystallogr F Struct Biol. 2022 Jan 1;78(Pt 1):39-44.
Hiltunen MK, Beyer HM, Iwaï H. Mini-Intein Structures from Extremophiles Suggest a Strategy for Finding Novel Robust Inteins. Microorganisms. 2021 Jun 5;9(6):1226. doi: 10.3390/microorganisms9061226. PMID: 34198729.
Colombo J, Antkowiak A, Kogan K, Kotila T, Elliott J, Guillotin A, Lappalainen P, Michelot A. A functional family of fluorescent nucleotide analogues to investigate actin dynamics and energetics. Nat Commun. 2021 Jan 22;12(1):548. doi: 10.1038/s41467-020-20827-4. PMID: 33483497.
Hsu SD, Lee YC, Mikula KM, Backlund SM, Tascón I, Goldman A, Iwaï H. Tying up the Loose Ends: A Mathematically Knotted Protein. Front Chem. 2021 May 24;9:663241. doi: 10.3389/fchem.2021.663241. PMID: 34109153; PMCID: PMC8182377.
Beyer HM, Virtanen SI, Aranko AS, Mikula KM, Lountos GT, Wlodawer A, Ollila OHS, Iwaï H. The Convergence of the Hedgehog/Intein Fold in Different Protein Splicing Mechanisms. Int J Mol Sci. 2020 Nov 7;21(21):8367. doi: 10.3390/ijms21218367. PMID: 33171880; PMCID: PMC7664689.
Leppänen VM, Brouillard P, Korhonen EA, Sipilä T, Jha SK, Revencu N, Labarque V, Fastré E, Schlögel M, Ravoet M, Singer A, Luzzatto C, Angelone D, Crichiutti G, D'Elia A, Kuurne J, Elamaa H, Koh GY, Saharinen P, Vikkula M, Alitalo K. Characterization of ANGPT2 mutations associated with primary lymphedema. Sci Transl Med. 2020 Sep 9;12(560):eaax8013. doi: 10.1126/scitranslmed.aax8013. PMID: 32908006.
Karki S, Shkumatov AV, Bae S, Kim H, Ko J, Kajander T. Structural basis of SALM3 dimerization and synaptic adhesion complex formation with PTPσ. Sci Rep. 2020 Jul 14;10(1):11557. doi: 10.1038/s41598-020-68502-4. PMID: 32665594; PMCID: PMC7360590.
Beyer HM, Mikula KM, Li M, Wlodawer A, Iwaï H. The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins. FEBS J. 2020 May;287(9):1886-1898. doi: 10.1111/febs.15113.
Kotila, T., Wioland, H., Enkavi, G., Kogan, K., Vattulainen, I., Jégou, A., Romet-Lemonne, G., Lappalainen, P. Mechanism of synergistic actin filament pointed end depolymerization by cyclase-associated protein and cofilin. Nat Commun 10, 5320 (2019) doi:10.1038/s41467-019-13213-2.
Mikula KM, Kolodziejczyk R, Goldman A. Structure of the UspA1 protein fragment from Moraxella catarrhalis responsible for C3d binding. J Struct Biol. 2019 Aug 7. pii: S1047-8477(19)30172-8. doi: 10.1016/j.jsb.2019.08.002.
Beyer HM, Mikula KM, Kudling TV, Iwaï H. Crystal structures of CDC21-1 inteins from hyperthermophilic archaea reveal the selection mechanism for the highly conserved homing endonuclease insertion site. Extremophiles. 2019 Jul 30. doi:10.1007/s00792-019-01117-4.
Vidilaseris K, Kiriazis A, Turku A, Khattab A, Johansson NG, Leino TO, Kiuru PS, Boije Af Gennäs G, Meri S, Yli-Kauhaluoma J, Xhaard H, Goldman A. Asymmetry in catalysis by Thermotoga maritima membrane-bound pyrophosphatase demonstrated by a nonphosphorus allosteric inhibitor. Sci Adv. 2019 May 22;5(5):eaav7574. doi:10.1126/sciadv.aav7574. eCollection 2019 May.
Wang L, Yan Z, Vihinen H, Eriksson O, Wang W, Soliymani R, Lu Y, Xue Y, Jokitalo E, Li J, Zhao H. FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function. J Cell Biol. 2019 Jan 7;218(1):97-111.
Medarametla P, Gatta V, Kajander T, Laitinen T, Tammela P, Poso A. Structure-Based Virtual Screening of LsrK Kinase Inhibitors to Target Quorum Sensing. ChemMedChem. 2018 Nov 20;13(22):2400-2407. doi: 10.1002/cmdc.201800548. Epub 2018 Oct 30.
Richardson D, Itkonen J, Nievas J, Urtti A, Casteleijn MG. Accelerated pharmaceutical protein development with integrated cell free expression, purification, and bioconjugation. Sci Rep. 2018 Aug 10 ; 8(1):11967.
Kotila T, Kogan K, Enkavi G, Guo S, Vattulainen I, Goode BL, Lappalainen P. Structural basis of actin monomer re-charging by cyclase-associated protein. Nat Commun. 2018 May 14;9(1):1892. doi: 10.1038/s41467-018-04231-7.
Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T. The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition. ProteinEng Des Sel. 2018 May 1;31(5):147-157. doi: 10.1093/protein/gzy012.
Kolodziejczyk R, Mikula KM, Kotila T, Postis VLG, Jokiranta TS, Goldman A, Meri T. Crystal structure of a tripartite complex between C3dg, C-terminal domains of factor H and OspE of Borrelia burgdorferi. PLoS One. 2017 Nov 30;12(11):e0188127. doi: 10.1371/journal.pone.0188127.
Iwaï H, Mikula KM, Oeemig JS, Zhou D, Li M, Wlodawer A. Structural Basis for the Persistence of Homing Endonucleases in Transcription Factor IIB Inteins. J Mol Biol. 2017 Dec 8;429(24):3942-3956. doi: 10.1016/j.jmb.2017.10.016.
Leppänen VM, Saharinen P, Alitalo K. Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization. Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):4376-4381. doi: 10.1073/pnas.1616166114.
Li KM, Wilkinson C, Kellosalo J, Tsai JY, Kajander T, Jeuken LJ, Sun YJ, Goldman A. Membrane pyrophosphatases from Thermotoga maritima and Vigna radiata suggest a conserved coupling mechanism. Nat Commun. 2016 Dec 6;7:13596. doi: 10.1038/ncomms13596.
Paatero A, Rosti K, Shkumatov AV, Sele C, Brunello C, Kysenius K, Singha P, Jokinen V, Huttunen H, Kajander T. Crystal Structure of an Engineered LRRTM2 Synaptic Adhesion Molecule and a Model for Neurexin Binding. Biochemistry. 2016 Feb 16;55(6):914-26. doi: 10.1021/acs.biochem.5b00971. Epub 2016 Feb 3.
Guryanov SG, Liljeroos L, Kasaragod P, Kajander T, Butcher SJ. Crystal Structure of the Measles Virus Nucleoprotein Core in Complex with an N-Terminal Region of Phosphoprotein. J Virol. 2015 Dec 30;90(6):2849-57. doi: 10.1128/JVI.02865-15.
Shakeel S., Westerhuis B.M., Ora, A., Koen, G., Bakker, A.Q., Claassen, Y., Wagner, K., Beaumont, T., Wolthers, K.C., Butcher, S.J. (2015) Structural basis of human parechovirus neutralization by human monoclonal antibodies. J. Virol. 89:9571-80. doi: 10.1128/JVI.01429-15.
Bhattacharjee A, Reuter S, Trojnár E, Kolodziejczyk R, Seeberger H, Hyvärinen S, Uzonyi B, Szilágyi Á, Prohászka Z, Goldman A, Józsi M, Jokiranta TS. The major autoantibody epitope on factor H in atypical hemolytic uremic syndrome is structurally different from its homologous site in factor H-related protein 1, supporting a novel model for induction of autoimmunity in this disease. J Biol Chem. 2015 Apr 10;290(15):9500-10. doi: 10.1074/jbc.M114.630871. Epub 2015.
Rosti K, Goldman A, Kajander T. Solution structure and biophysical characterization of the multifaceted signalling effector protein growth arrest specific-1. BMC Biochem. 2015 Feb 28;16:8. doi: 10.1186/s12858-015-0037-6.
Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwaï H. Structure-based engineering and comparison of novel split inteins for protein ligation. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
Aranko AS, Oeemig JS, Kajander T, Iwaï H. Intermolecular domain swapping induces intein-mediated protein alternative splicing. Nat Chem Biol. 2013 Oct;9(10):616-22. doi: 10.1038/nchembio.1320. Epub 2013 Aug 25.
Happonen LJ, Oksanen E, Liljeroos L, Goldman A, Kajander T, Butcher SJ. The structure of the NTPase that powers DNA packaging into Sulfolobus turreted icosahedral virus 2. J Virol. 2013 Aug;87(15):8388-98. doi: 10.1128/JVI.00831-13. Epub 2013 May 22.
Bhattacharjee A, Oeemig JS, Kolodziejczyk R, Meri T, Kajander T, Lehtinen MJ, Iwaï H, Jokiranta TS, Goldman A. Structural basis for complement evasion by Lyme disease pathogen Borrelia burgdorferi. J Biol Chem. 2013 Jun 28;288(26):18685-95. doi: 10.1074/jbc.M113.459040.
Leppänen VM, Tvorogov D, Kisko K, Prota AE, Jeltsch M, Anisimov A, Markovic-Mueller S, Stuttfeld E, Goldie KN, Ballmer-Hofer K, Alitalo K. Structural and mechanistic insights into VEGF receptor 3 ligand binding and activation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):12960-5. doi: 10.1073/pnas.1301415110.
Pihlajamaa T, Kajander T, Knuuti J, Horkka K, Sharma A, Permi P. Structure of Plasmodium falciparum TRAP (thrombospondin-related anonymous protein) A domain highlights distinct features in apicomplexan von Willebrand factor A homologues. Biochem J. 2013 Mar 15;450(3):469-76. doi: 10.1042/BJ20121058.
Kellosalo J, Kajander T, Honkanen R, Goldman A. Crystallization and preliminary X-ray analysis of membrane-bound pyrophosphatases. Mol Membr Biol. 2013 Feb;30(1):64-74. doi: 10.3109/09687688.2012.712162. Epub 2012 Aug 13.
Kellosalo J, Kajander T, Kogan K, Pokharel K, Goldman A. The structure and catalytic cycle of a sodium-pumping pyrophosphatase. Science. 2012 Jul 27;337(6093):473-6. doi: 10.1126/science.1222505.
Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwaï H. NMR and crystal structures of the Pyrococcus horikoshii RadA intein guide a strategy for engineering a highly efficient and promiscuous intein. J Mol Biol. 2012 Aug 3;421(1):85-99. doi: 10.1016/j.jmb.2012.04.029. Epub 2012 May 2.
Kajander T, Kuja-Panula J, Rauvala H, Goldman A. Crystal structure and role of glycans and dimerization in folding of neuronal leucine-rich repeat protein AMIGO-1. J Mol Biol. 2011 Nov 11;413(5):1001-15. doi: 10.1016/j.jmb.2011.09.032. Epub 2011 Sep 29.