Instruct-HiLIFE Applications

Biocomplex provides purification technologies and expertise specializing in the ultracentrifugation techniques, monolithic chromatography and asymmetrical flow field-flow fractionation (AF4). Provided techniques can be used in different combinations to yield high quality samples in quantity.

• Separation and purification of cells, organelles, macromolecules, macromolecular complexes, vesicles and viruses etc.
• Ultracentrifugation combined with swing-out and fixed-angle rotors of different capacities;
• Monolithic ion exchange chromatography;
• Asymmetrical flow field-flow fractionation (AF4);
• User training, assisted use of instruments, open access for trained users, specialist support and maintenance;
• Project planning;
• Full service packages on sample purification (including analysis of the quality and quantity);
• Collaborative projects

• Sample preparation for crystallisation
• Pre-crystallisation tests
• Screens for protein stability
• Temperature dependence of three-dimensional crystal formation of protein
• Protein crystallisation, membrane proteins especially
• Screens for crystallisation conditions: random, grid and targeted screens
• Protein optimisation using fluorescence-based thermal shift assay
• Optimisation of crystallisation hits
• Collaborative projects
• Education and training in protein crystallography
• Help with the structure determination and analysis

Cryo-EM is applicable for projects in e.g. structural biology, nanotechnology, time-resolved assembly and phase transitions. Depending on the approach used, subnanometer resolution can be achieved.

• Single particle averaging and three dimensional (3D) reconstructions
• Electron tomography and 3D-reconstructions
• Measurements and statistical analyses
• Study of biological macromolecular complexes and other electron-beam sensitive materials in aqueous solutions
• Particle sizes that range from ~10 to 300 nm in diameter
• Project planning and assessment
• Sample optimisation and preparation
• Plunge freezing services (BSL2)
• Advice on image reconstruction
• Collaborative projects
• Courses in structural biology, cryo electron microscopy and image reconstruction

• Structure determination of a protein up to 30 kDa in native liquid state
• Protein-ligand and protein-protein interactions in liquid state
• Dynamic of a macromolecule characterization over a wide range of time scales
• Macromolecule size determination with Diffusion spectroscopy
• Protein backbone dynamics analysis
• Effects of a mutation to structure
• Small molecule NMR