Instruct-HiLIFE Applications

Biocomplex provides purification technologies and expertise specializing in the ultracentrifugation techniques, monolithic chromatography and asymmetrical flow field-flow fractionation (AF4). Provided techniques can be used in different combinations to yield high quality samples in quantity.

  • Separation and purification of cells, organelles, macromolecules, macromolecular complexes, vesicles and viruses etc.
  • Ultracentrifugation combined with swing-out and fixed-angle rotors of different capacities;
  • Monolithic ion exchange chromatography;
  • Asymmetrical flow field-flow fractionation (AF4);
  • User training, assisted use of instruments, open access for trained users, specialist support and maintenance;
  • Project planning;
  • Full service packages on sample purification (including analysis of the quality and quantity);
  • Collaborative projects
  • Sample preparation for crystallisation
  • Pre-crystallisation tests
  • Screens for protein stability
  • Temperature dependence of three-dimensional crystal formation of protein
  • Protein crystallisation, membrane proteins especially
  • Screens for crystallisation conditions: random, grid and targeted screens
  • Protein optimisation using fluorescence-based thermal shift assay
  • Optimisation of crystallisation hits
  • Collaborative projects
  • Education and training in protein crystallography
  • Help with the structure determination and analysis

Cryo-EM is applicable for projects in e.g. structural biology, nanotechnology, time-resolved assembly and phase transitions. Depending on the approach used, subnanometer resolution can be achieved.

  • Single particle averaging and three dimensional (3D) reconstructions
  • Electron tomography and 3D-reconstructions
  • Measurements and statistical analyses
  • Study of biological macromolecular complexes and other electron-beam sensitive materials in aqueous solutions
  • Particle sizes that range from ~10 to 300 nm in diameter
  • Project planning and assessment
  • Sample optimisation and preparation
  • Plunge freezing services (BSL2)
  • Advice on image reconstruction
  • Collaborative projects
  • Courses in structural biology, cryo electron microscopy and image reconstruction
  • Structure determination of a protein up to 30 kDa in native liquid state
  • Protein-ligand and protein-protein interactions in liquid state
  • Dynamic of a macromolecule characterization over a wide range of time scales
  • Macromolecule size determination with Diffusion spectroscopy
  • Protein backbone dynamics analysis
  • Effects of a mutation to structure
  • Small molecule NMR