Biomedicum Stem Cell Center

The Biomedicum Stem Cell Center (BSCC) core facility is a comprehensive provider of human pluripotent stem cell services at the Meilahti campus of the University of Helsinki. iPSC-derived, disease specific cell models serve as an opportunity to study pathophysiological mechanisms and can lead to development of new treatments. We provide locally derived human pluripotent stem cell lines and generate induced pluripotent stem cell (iPSC) lines from somatic cells provided by the client. BSCC operates on a fee-for-service basis in providing custom iPSC derivation using the latest technologies. BSCC is a part of the life science infrastructure of HiLife GoEditStem platform and the Stem Cells and Biomaterials consortium, national Biocenter Finland technology platform.

BSCC is equipped with basic cell culture equipment such as laminars, incubators, centrifuges and microscopes. For further information, please visit https://www.helsinki.fi/en/researchgroups/pluripotency-and-disease-modeling/bscc-core-facility

Contact person: BSCC Director Timo Otonkoski, timo.otonkoski@helsinki.fi or BSCC Coordinator Ras Trokovic, ras.trokovic@helsinki.fi

Location: Biomedicum Helsinki, Biomedicum Stem Cell Center, 5th floor, Haartmaninkatu 8, Meilahti Campus

Services

  • Cell distribution
  • Derivation and characterization of iPSC lines
  • User training and education
  • Miscellaneous stem cell research services
  • Genome editing, on demand
  • Differentiation into endodermal lineages, on demand

Instruments

  • Thermo Forma Water Jacketed CO2 Incubator (2 x)
  • Heraeus Multifuge 3S-R (w/ tube and plate rotor)
  • Grant waterbath
  • Sartorius Biohit MidiPlus pipettors
  • Stuart vortex
  • Kojair Biowizard Biosafety cabinet
  • 4D-Nucleofector™
  • Eppendorf Centrifuge 5702

Applications

  • Distribution of locally derived human pluripotent stem cell lines
  • Non-integrative technologies for reprogramming of client provided somatic cells
  • Customized training and education

User fees

The research infrastructure is supported by University of Helsinki (HiLIFE) and Biocenter Finland. The prices are listed below. 

Product Volume University of Helsinki price (VAT 0) eur Academic price (not included VAT) eur Non-academic price (not incl. VAT) eur
Use of Cell IQ 1 plate/h  2 -  
Frozen hPSC 1 ampoule 120 -  
Thawed hPSC 1 plate 200 -  
Culture of hPSC passage 50 -  
hiPSC generation from fibroblasts 2 characterized clones, frozen at passage ten and 2-4 uncharacterized clones frozen at passage 3 2200 2983 3233
hiPSC generation from blood 2 characterized clones, frozen at passage ten and 2-4 uncharacterized clones frozen at passage 3 2800 3720 4005
Early hiPSC generation from fibroblasts 4-6 uncharacterized clones frozen at passage 3 1000    
Early hiPSC generation from blood 4-6 uncharacterized clones frozen at passage 3 1800    
Fibroblast culture from skin biopsy for hiPSC generation   300 -  
DNA Fingerprint analysis 1 cell line 120 -  
Miscellaneous stem cell research services 1 hour 44 44 60
Genome editing Generation of knockouts within GoEditStem platform (HiLIFE)  On demand    
Differentiation into endodermal lineages   On demand