FIMM Genomics

FIMM Genomics provides open-access DNA/RNA sequencing and genotyping services for researchers in Finland and abroad. Our primary focus is in medical genomics and transcriptomics including primary analysis of the sequencing data.
We help you design experiments and provide tailored solutions for basic and translational research. Our experienced staff is ready to implement novel technologies and assays to match your needs.

Contacts:

Head of sequencing unit Pekka Ellonen+358 50 415 4481
pekka.ellonen(ät)fimm.fi

NGS Transcriptomics Pirkko Mattila+358 50 317 5434
pirkko.mattila(ät)fimm.fi

Head of genotyping unit Kati Donner+358 50 318 5677
kati.donner(ät)fimm.fi

Sequencing laboratory website

Genotyping laboratory website

Location:

Biomedicum Helsinki 2, Tukholmankatu 8, Meilahti campus, Helsinki
2nd floor, F-wing

Services:

Exome Sequencing
Whole Genome Sequencing
Transcriptome sequencing
Gene Panels & Custom Gene panels
Tailored NGS amplicons / biomarkers
NGS library preparation
T-cell receptor sequencing (hsTCRB)
NGS target enrichment / custom gene panels

Custom NGS assay development - ask more

Whole Genome Genotyping
Whole Genome Methylation analysis
Targeted SNP Genotyping
Targeted Methylation analysis
Cell line authentication
Mouse speed congenics
Illumina NovaSeq6000 sequencing
Illumina HiSeq2500 sequencing
Illumina MiSeq sequencing

Sanger Sequencing & Fragment Analysis (ABI3730xl DNA Analyzer)

Consultation and project planning

Instruments:

Illumina NovaSeq6000
Illumina HiSeq2500 
Illumina HiSeq2500 
Illumina HISeq1500
Illumina MiSeq

ABI3730xl DNA Analyzer

10XGenomics Chromium Controller
Illumina iScan
Agena Bioscience

2100 Agilent Bioanalyzer
LabChip GXII Touch HT
Sage Science BluePippin
Covaris E220e AFA sonicator

User fees:

The research infrastructure is supported by University of Helsinki (HiLIFE) and Biocenter Finland. Service pricing follows University of Helsinki pricing guidelines.

Below some examplary prices are shown (University of Helsinki internal pricing, VAT0%). We will tailor NGS and genotyping services for your needs and pricing will vary accordingly. Please contact us for experiment design and pricing details.

Exome examples:

Exome sequencing, germline starting from 770e/sample mean coverage ~50X, 90% target bases with at least 20X coverage Price varies depending on the desired sequencing depth and preferred exome kit Primary analysis included (FASTQ, BAM, VCF files)
Exome sequencing, somatic starting from 1124 €/sample mean coverage ~130X,90% target bases with at least 50X coverage Price varies depending on the desired sequencing depth and preferred exome kit Primary analysis included (FASTQ, BAM, VCF files)

NGS library preparations for DNA  are about 50..100€/sample. Price varies depending on NGS application, kit vendor, sample type and amount of DNA available

Transcriptome 165.61€ Scriptseqv2 ~60M reads of sequencing required, see sequencing run modes below Amount of sequencing can be adjusted to project needs
Gene expression /3´counting 51.64 € Lexogen QuantSeq ~5M reads of sequencing required, see sequencing run modes below Amount of sequencing can be adjusted to project needs
smallRNA-seq / miRnome 136.72 € TruSeq smallRNA ~15M reads of sequencing required, see sequencing run modes below Amount of sequencing can be adjusted to project needs

Sequencing

Frequently requested sequencing run modes:

Platform

SBS version Mode Reads total (M) Typical Yield (Gb) Yield after barcode removal (Gb) HY price / lane Cost/1M reads
Illumina HiSeq HO v4 v4 PE100 475 47.5 43.7 €2,037.33 4.05€
Illumina HiSeq Rapid Rapid v2 PE100 532 55 50.7 €3,291.20 6.18€
Illumina HiSeq Rapid Rapid v2 PE250 532 133 128.7 5 925.20 € 11.13€
Illumina MiSeq v2 PE150 v2 32 4.7 4.4 1 249.12 € 42.10€
Illumina MiSeq v2 PE250 v2 32 8 7.7 €1,367.12 45.78€
Illumina MiSeq v3 PE300 v3 50 15 14.6 €1,754.12 45.78€

For list of all available sequencing modes please browse sequencing instruments above or contact laboratory staff.

Yields are average yield from manufacturer's specifications. Real life performance varies depending on sample and library type and quality.

Genotyping

Global screening array 20-32 € per sample ~640 000 markers

For projects with ≤4608 samples the price is 32 € per sample. For projects with >4608 samples the price is 29 € per sample.