Guidelines

The Flow Cytometry Unit provides flow cytometry analysis and cell sorting services as well as user training for new users of the instruments. Access to the flow cytometers and their reservation calenders for individual users is given after a training session. Training for new users is arranged regularly.

New users should contact the corresponding unit's personnel and discuss their experimental setup before their first reservation. When making a contact, please provide the following information: Full name, e-mail address and phone number, Department or research group, Principal investigator or supervisor (name and address), Billing information (profit center and WBS codes).

New independent users should be acquainted to basic flow cytometry before the user training. We provide all users with practical guidance for working in the unit. Appropriate training for proper use of the instruments is obligatory for all new users. Contact the corresponding unit´s personnel for further information and reservations for training.

Reservation system is still available based on current procedures (check individual infrastructure web pages). A centralised online reservation system,  will be lauched soon. Userprofiles will be granted by the personnel.

The guidelines might vary between the units. Please contact the corresponding unit's personnel and/or web pages for more information.

Reservation: Remeber to do reservations prior to instrument usage and possible cancellations as early as possible. Uncancelled reservations will be charged (see user fees).

Instrument: The user should ensure that the instrument is ready to use with sufficient liquids and waste. Take care that all necessary start up and shut down procedures are done according to instrument guidelines if needed. If you make any special changes to the instrument settings, change filters etc., please return the setup to the starting state. Remember to fill the logbook.

The unit: In case of problems or need of help, contact the personnel. Please, inform the personnel if you notice any of the solutions or disposables running low in the lab. Remember to take copies of all you data (including the fcs files). Virus scanning must be done every time before you may attach your external memory to any computer.

Laboratory Safety:  Normal laboratory safety procedures should be followed. No eating or drinking in the laboratory. 

Flow Cytometer safety:  Flow book

Samples: Contact the unit for more precis instructions. The general rules are:

  • No infectious or pathogenic samples are allowed
  • Human blood samples should be tested.
  • No viruses,  all cell lines transducted with virus (e.g. lentiviral vectors) has to be certified RCV negative. (you can visit FuGu)
  • Bacteria: discuss with personnel.
  • iPS: discuss with personnel.

Acknowledgements: Please note that we expect you to acknowledge the unit when publishing results using any of the Flow unit instruments or services. e.g. “The flow cytometry analysis was performed at the HiLife Flow Cytometry Unit, University of Helsinki.” Kindly inform the Flow unit personnel about your publication.

 

INSTRUMENTS

€ / h

Training

Additional fees

ANALYZERS

Beckman Coulter Gallios (Biomedicum) 25€ 200€ 30€/h for service run
BD Accuri (Biomedicum) 25€ 150€ 30€/h for service run
BD LSRFortessa (Biocenter) 25€  60€/h 10€ start up, 30€/h for service
BD LSRII (Biocenter) 20€ 60€/h 30€/h for service run

SORTERS

FACSAria II (Biomedicum) 30€ 400€ 30€/h for service run
FACSAria II (Viikki) 30€ 60€/h 30€/h for service run
Sony SH800z (Biomedicum) 30€ 200€ 30€/h for service run
BD Influx (Biomedicum) 60€* -  

OTHER

FlowJo software 5€ -  
GentleMACS Dissociator (Biomedicum)      tubes are invoiced

* price includes the operator, the instrument can only be used by Flow cytometry units personnel.

  • Prices valid for University of Helsinki customers.
  • For HUS users an addition of VAT 24 % will be applied.
  • For other academic and non-academic user fees, please, contact the corresponding facility.

Cancellation policy

  • Cancellations of sorters 24h before the starting time are not charged. Later cancellations are charged according to the booked time.
  • Cancellations of analyzators 12 h before the starting time are not charged. Later cancellations are charged according to the booked time.