FIMM HiPREP Single Cell Sample Preparation

FIMM HiPREP Single Cell Sample Preparation is a service of the FIMM Technology Centre.

We offer a well-equipped laboratory for researchers to prepare their own single cell suspension samples and run quality control before single cell analytics (bookings through ). We are part of HiLIFE Single Cell Omics platform and work in close collaboration with . For spatial transcriptomics quality control (Visium), we collaborate with  (request services via this ). In addition, we collaborate with . 

 

New customers or projects

For more information, prices and quotes, please see below and contact the  /

 

Access to the single cell sample preparation laboratory 

The single cell sample preparation laboratory is located next to FIMM Single-Cell analytics core enabling quick access to sequencing after sample preparation. New customers can apply for a usership and book the laboratory through the . Instructions to the OpenIRIS can be found here (). HiPREP personnel trains all new users before starting. Access key to the room can be obtained from the HiPREP core and should be returned after the work is finished. 

 

Spatial transcriptomics quality control service

Spatial transcriptomics (Visium) is a high-end technique that requires stringent quality control before starting the actual experiment. For performing the quality control of fresh frozen or FFPE tissues, you can request sectioning, RNA-isolation and quality control services via this . Sectioning is performed at the , with direct delivery of the sections for RNA isolation and quality control at the FIMM HiPREP Core.
Equipment

gentleMACS Octo Dissociator

  • Standardized, fully automated and heated (cooling) dissociation or homogenization of tissues
  • Designed to obtain highly viable single-cells or single-nuclei suspensions
  • Capacity 8 samples in one run

 

Luna FL Cell Counter (Logos Biosystems)

  • Automated cell counting and cell viability analysis
  • Suitable for different cell types and sizes
  • Dual fluorescence and brightfield optics

 

 

LeviCell 1.0 (Levitasbio)

  • Separation of live single cells from dead cells and debris
  • Single-use cartridges
  • Low pressure fluidics with low shear stress
  • Incorporated image capture and analysis software

 

Digital stereomicroscope DM-143-FBGG-C

  • Sample cutting and cell visualization
  • Binocular head
  • Built-in digital camera
  • Magnifications from 1X to 4X

 

 

Olympus BH-2 microscope

  • Cell visualization
  • Digital camera from  stereomicroscope can be attached
  • Magnifications 10X, 20X, 40X, 50X, 100X

 

 

Leitz Labovert inverted microscope

  • Cell visualization
  • Magnifications 6.3X, 10X, 20X, 32X

 

 

Kajor Biosafety cabinet

  • Class II cabinet available for sample preparation 

 

 

 

Roto-shake Genie

  • Rocking, rotating and 3-D orbital shaker
  • Rocking tray accommodates eppendorfs, 15-50 ml falcon tubes and larger open containers

 

 

Eppendorf 5425 R centrifuge

  • -10°C - +40°C
  • 21 300 x g
  • 0.5-1.5-2.0 ml tubes

 

 

Eppendorf 5804 centrifuge (swing-out bucket)

  • 15°C-35°C
  • 20 913 x g
  • 15 ml and 50 ml tubes

 

 

Grant Thermal BeadBath

  • Heating up to 95°C
  • Bottles, 15 ml and 50 ml tubes, microcentrifuge tubes

 

 

 

Eppendorf ThermoMixer C thermoblock

  • mixing, heating, cooling
  • SmartBlocks: 1.5-2.0 ml microcentrifuge tubes, 15 ml falcons

 

 

Other equipment

  • Orbital shaker (Rotamax 120, Heidolph)
  • Microcentrifuge (1,5 ml and 2 ml microcenrifuge tubes)
  • Vortex