Acknowledgements and publications

Our performance evaluation is based on the quality and quantity of research publications that our work has supported. Thus, to help us secure the support and maintain the services also in the future, we kindly ask you to acknowledge us in publications resulting from work done at our units. Please remember to acknowledge us also for the work done in multi-unit pipelines and big consortia projects.

How to acknowledge the HiPREP Core

How to acknowledge the HiPREP Core for nucleic acid or cell services?

"The authors would like to thank HiPREP Core at FIMM Technology Centre supported by HiLIFE and Biocenter Finland for [type of] services."

"[type of] service was performed at HiPREP Core in FIMM Technology Centre supported by HiLIFE and Biocenter Finland."

How to acknowledge the EV and HiPREP Core for EV services?

"We acknowledge services of the University of Helsinki: EV Core in Viikki for [type of] service and HiPREP Core in FIMM Technology Centre supported by HiLIFE and Biocenter Finland for [type of] service."

In case of EM services for EVs:

"We acknowledge services of the University of Helsinki: HiPREP Core in FIMM Technology Centre for electron microscopy work and Electron Microscopy Unit of the Institute of Biotechnology for providing the facilities supported by HiLIFE and Biocenter Finland.”

Selected scientific publications

Including authors of the HiPREP Core or collaborating EV Core

2025

EATRIS-Plus Multi-omics working group and Stakeholder, Alonso-Andres, P., Baldazzi, D., Chen, Q., Donner, K., Ghimire, B., Hannula, S., Lindgren, H., Mattila, P., Nazir, K., Nieminen, A.I., Palva, A., Puhka, M., Quintero, I., Scherer, A., Yadav, B. & Zizkovicova, P., 2025. Multi-omics quality assessment in personalized medicine through European infrastructure for translational medicine (EATRIS): An overview. Phenomics, 15, pp. 1–15.

Joo, S., Dhaygude, K., Krebs, R., Holmström, E., Puhka, M., Laitinen, S., Dellgren, G., Magnusson, J., Holmberg, E.C., Svahn, J., Lund, T.K., Leuckfeld, I., Raivio, P., Helanterä, I., Åberg, F., Syrjälä, S.O., Nykänen, A.I. and Lemström, K.B., 2025. Extracellular vesicles in lung donor plasma: Potential indicators of donor organ quality. Journal of Thoracic and Cardiovascular Surgery [online ahead of print PMID: 40118141].

Varik, I., Saretok, K.J., Rosenberg, K., Quintero, I., Puhka, M., Volkova, N., Trošin, A., Guazzi, P. and Velthut-Meikas, A., 2025. Small and large extracellular vesicles from human preovulatory follicular fluid display distinct ncRNA cargo profile and differential effect on granulosa cell line KGN. bioRxiv. https://doi.org/10.1101/2025.02.13.638014

2024

Barreiro, K., Karttunen, J., Valo, E., Viippola, E., Quintero, I., Käräjämäki, A., Rannikko, A., Holthöfer, H., Ganna, A., Sandholm, N., Thorn, L.M., Groop, P.H., Tuomi, T., Dwivedi, O.P. and Puhka, M., 2024. Selected miRNAs in urinary extracellular vesicles show promise for non-invasive diagnostics of diabetic kidney disease. medRxiv. https://doi.org/10.1101/2024.09.12.24312889

Clotet-Freixas, S., Zaslaver, O., Kotlyar, M., Pastrello, C., Quaile, A.T., McEvoy, C.M., Saha, A.D., Farkona, S., Boshart, A., Zorcic, K., Neupane, S., Manion, K., Allen, M., Chan, M., Chen, X., Arnold, A.P., Sekula, P., Steinbrenner, I., Köttgen, A., Dart, A.B., Wicklow, B., McGavock, J.M., Blydt-Hansen, T.D., Barrios, C., Riera, M., Soler, M.J., Isenbrandt, A., Lamontagne-Proulx, J., Pradeloux, S., Coulombe, K., Soulet, D., Rajasekar, S., Zhang, B., John, R., Mehrotra, A., Gehring, A., Puhka, M., Jurisica, I., Woo, M., Scholey, J.W., Röst, H. and Konvalinka, A., 2024. Sex differences in kidney metabolism may reflect sex-dependent outcomes in human diabetic kidney disease. Science Translational Medicine, 16(737), eabm2090. https://doi.org/10.1126/scitranslmed.abm2090

Hörtenhuber, M., Hytönen, M.K., Mukarram, A.K., Arumilli, M., Araujo, C.L., Quintero, I., Syrjä, P., Airas, N., Kaukonen, M., Kyöstilä, K., Niskanen, J., Jokinen, T.S., Mottaghitalab, F., Takan, I., Salokorpi, N., Raman, A., Stevens, I., Iivanainen, A., Yoshihara, M., Gusev, O., Bannasch, D., Sukura, A., Schoenebeck, J.J., DoGA Consortium, Ezer, S., Katayama, S., Daub, C.O., Kere, J. and Lohi, H., 2024. The DoGA consortium expression atlas of promoters and genes in 100 canine tissues. Nature Communications, 15(1), p.9082. https://doi.org/10.1038/s41467-024-52798-1

Khan, M., Islam, M.K., Rahman, M., Dhondt, B., Quintero, I., Puhka, M., Jaakkola, P.M., Lamminmäki, U. and Leivo, J., 2024. Tetraspanin immunoassay for the detection of extracellular vesicles and renal cell carcinoma. NanoSelect. [online first] https://doi.org/10.1002/nano.202400123 (Note: Insert correct DOI if available.)

Nieuwland, R. and Siljander, P.R., 2024. A beginner's guide to study extracellular vesicles in human blood plasma and serum. Journal of Extracellular Vesicles, 13(1), e12400. https://doi.org/10.1002/jev2.12400

Palviainen, M., Puutio, J., Østergaard, R.H., Eble, J.A., Maaninka, K., Butt, U., Ndika, J., Kari, O.K., Kamali-Moghaddam, M., Kjaer-Sorensen, K., Oxvig, C., Aransay, A.M., Falcon-Perez, J.M., Federico, A., Greco, D., Laitinen, S., Hayashi, Y. and Siljander, P.R., 2024. Beyond basic characterization and omics: Immunomodulatory roles of platelet-derived extracellular vesicles unveiled by functional testing. Journal of Extracellular Vesicles, 13(10), e12513. https://doi.org/10.1002/jev2.12513

Tavukcuoglu, Z., Butt, U., Faria, A.V.S., Oesterreicher, J., Holnthoner, W., Laitinen, S., Palviainen, M. and Siljander, P.R., 2024. Platelet-derived extracellular vesicles induced through different activation pathways drive melanoma progression by functional and transcriptional changes. Cell Communication and Signaling, 22(1), p.601. https://doi.org/10.1186/s12964-024-01973-4

2023

Barreiro, K., Dwivedi, O.P., Rannikko, A., Holthöfer, H., Tuomi, T., Groop, P.H. and Puhka, M., 2023. Capturing the kidney transcriptome by urinary extracellular vesicles—from pre-analytical obstacles to biomarker research. Genes (Basel), 14(7), p.1415. https://doi.org/10.3390/genes14071415

Barreiro, K., Lay, A.C., Leparc, G., Tran, V.D.T., Rosler, M., Dayalan, L., Burdet, F., Ibberson, M., Coward, R.J.M., Huber, T.B., Krämer, B.K., Delic, D. and Holthöfer, H., 2023. An in vitro approach to understand contribution of kidney cells to human urinary extracellular vesicles. Journal of Extracellular Vesicles, 12(2), e12304. https://doi.org/10.1002/jev2.12304

Dwivedi, O.P., Barreiro, K., Käräjämäki, A., Valo, E., Giri, A.K., Prasad, R.B., Roy, R.D., Thorn, L.M., Rannikko, A., Holthöfer, H., Gooding, K.M., Sourbron, S., Delic, D., Gomez, M.F., iBEAt study, Groop, P.H., Tuomi, T., Forsblom, C., Groop, L. and Puhka, M., 2023. Genome-wide mRNA profiling in urinary extracellular vesicles reveals stress gene signature for diabetic kidney disease. iScience, 26(5), p.106686. https://doi.org/10.1016/j.isci.2023.106686

Joo, S., Dhaygude, K., Westerberg, S., Krebs, R., Puhka, M., Holmström, E., Syrjälä, S., Nykänen, A.I. and Lemström, K., 2023. Transcriptomic landscape of circulating extracellular vesicles in heart transplant ischemia–reperfusion. Genes, 14(11), p.2101. https://doi.org/10.3390/genes14112101

Maaninka, K., Neuvonen, M., Kerkelä, E., Hyvärinen, K., Palviainen, M., Kamali-Moghaddam, M., Federico, A., Greco, D., Laitinen, S., Öörni, K. and Siljander, P.R., 2023. OxLDL sensitizes platelets for increased formation of extracellular vesicles capable of finetuning macrophage gene expression. European Journal of Cell Biology, 102(2), p.151311. https://doi.org/10.1016/j.ejcb.2023.151311

2022

Kyykallio, H., Faria, A.V.S., Hartmann, R., Capra, J., Rilla, K. and Siljander, P.R., 2022. A quick pipeline for the isolation of 3D cell culture-derived extracellular vesicles. Journal of Extracellular Vesicles, 11(10), e12273. https://doi.org/10.1002/jev2.12273

Nieuwland, R., Siljander, P.R., Falcón-Pérez, J.M. and Witwer, K.W., 2022. Reproducibility of extracellular vesicle research. European Journal of Cell Biology, 101(3), p.151226. https://doi.org/10.1016/j.ejcb.2022.151226

Puhka, M., Thierens, L., Nicorici, D., Forsman, T., Mirtti, T., af Hällström, T., Serkkola, E. and Rannikko, A., 2022. Exploration of extracellular vesicle miRNAs, targeted mRNAs and pathways in prostate cancer: Relation to disease status and progression. Cancers, 14(3), p.532. https://doi.org/10.3390/cancers14030532

Saraswat, M., Przybyla, B., Joenvaara, S., Tohmola, T., Strandin, T., Puhka, M., Jouppila, A., Lassila, R. and Renkonen, R., 2022. Urinary extracellular vesicles carry multiple activators and regulators of coagulation. Frontiers in Cell and Developmental Biology, 10, 967482. https://doi.org/10.3389/fcell.2022.967482

Winzely, M., Jouppila, A., Ramer, G., Lux, L., Lendl, B., Barreiro, K., Lassila, R. and Friedbacher, G., 2022. AFM investigation of APAC (antiplatelet and anticoagulant heparin proteoglycan). Analytical and Bioanalytical Chemistry, 414(2), pp.1029–1038. https://doi.org/10.1007/s00216-021-03765-y

2021

Barok, M., Puhka, M., Yazdi, N. and Joensuu, H., 2021. Extracellular vesicles as modifiers of antibody-drug conjugate efficacy. Journal of Extracellular Vesicles, 10(4), e12070. https://doi.org/10.1002/jev2.12070

Barreiro, K., Dwivedi, O.P., Rannikko, A., Holthöfer, H., Tuomi, T., Groop, P.H. and Puhka, M., 2023. Capturing the kidney transcriptome by urinary extracellular vesicles: From pre-analytical obstacles to biomarker research. Genes, 14(7), 1415. https://doi.org/10.3390/genes14071415

Barreiro, K., Dwivedi, O.P., Valkonen, S., Groop, P.H., Tuomi, T., Holthofer, H., Rannikko, A., Yliperttula, M., Siljander, P., Laitinen, S., Serkkola, E., af Hällström, T., Forsblom, C., Groop, L. and Puhka, M., 2021. Urinary extracellular vesicles: Assessment of pre-analytical variables and development of a quality control with focus on transcriptomic biomarker research. Journal of Extracellular Vesicles, 10(12), e12158. https://doi.org/10.1002/jev2.12158

Barreiro, K., Huber, T.B. and Holthofer, H., 2021. Urinary extracellular vesicles: Magic particles for biomarker discovery. In: Advances in Experimental Medicine and Biology, 1306, pp.29–40. https://doi.org/10.1007/978-3-030-63908-2_3

Clotet-Freixas, S., Zaslaver, O., Kotlyar, M., Pastrello, C., Quaile, A.T., McEvoy, C.M., Saha, A.D., Farkona, S., Boshart, A., Zorcic, K., Neupane, S., Manion, K., Allen, M., Chan, M., Chen, X., Arnold, A.P., Sekula, P., Steinbrenner, I., Köttgen, A., Dart, A.B., Wicklow, B., McGavock, J.M., Blydt-Hansen, T.D., Barrios, C., Riera, M., Soler, M.J., Isenbrandt, A., Lamontagne-Proulx, J., Pradeloux, S., Coulombe, K., Soulet, D., Rajasekar, S., Zhang, B., John, R., Mehrotra, A., Gehring, A., Puhka, M., Jurisica, I., Woo, M., Scholey, J.W., Röst, H. and Konvalinka, A., 2024. Sex differences in kidney metabolism may reflect sex-dependent outcomes in human diabetic kidney disease. Science Translational Medicine, 16(737), eabm2090. https://doi.org/10.1126/scitranslmed.abm2090

Maaninka, K., Neuvonen, M., Kerkelä, E., Hyvärinen, K., Palviainen, M., Kamali-Moghaddam, M., Federico, A., Greco, D., Laitinen, S., Öörni, K. & Siljander, P.R., 2023. OxLDL sensitizes platelets for increased formation of extracellular vesicles capable of finetuning macrophage gene expression. European Journal of Cell Biology, 102(2), p.151311. https://doi.org/10.1016/j.ejcb.2023.151311

Nevalainen, T., Autio, A., Puhka, M., Jylhä, M. and Hurme, M., 2021. Composition of the whole transcriptome in the human plasma: Cellular source and modification by aging. Experimental Gerontology, 143, 111119. https://doi.org/10.1016/j.exger.2020.111119

Nieuwland, R., Siljander, P.R., Falcón-Pérez, J.M. and Witwer, K.W., 2022. Reproducibility of extracellular vesicle research. European Journal of Cell Biology, 101(3), 151226. https://doi.org/10.1016/j.ejcb.2022.151226

Vestad, B., Nyman, T.A., Hove-Skovsgaard, M., Stensland, M., Hoel, H., Trøseid, A.S., Aspelin, T., Aass, H.C.D., Puhka, M., Hov, J.R., Nielsen, S.D., Øvstebø, R. and Trøseid, M., 2021. Plasma extracellular vesicles in people living with HIV and type 2 diabetes are related to microbial translocation and cardiovascular risk. Scientific Reports, 11(1), 21936. https://doi.org/10.1038/s41598-021-01334-y

2020

Barreiro, K., Dwivedi, O.P., Leparc, G., Rolser, M., Delic, D., Forsblom, C., Groop, P.H., Groop, L., Huber, T.B., Puhka, M. and Holthofer, H., 2020. Comparison of urinary extracellular vesicle isolation methods for transcriptomic biomarker research in diabetic kidney disease. Journal of Extracellular Vesicles, 10(2), e12038. https://doi.org/10.1002/jev2.12038

Barreiro, K., Huber, T.B. and Holthofer, H., 2020. Isolating urinary extracellular vesicles as biomarkers for diabetic disease. In: Methods in Molecular Biology, 2067, pp.175–188. https://doi.org/10.1007/978-1-4939-9841-8_13

Koponen, A., Kerkelä, E., Rojalin, T., Lázaro-Ibáñez, E., Suutari, T., Saari, H.O., Siljander, P., Yliperttula, M., Laitinen, S. and Viitala, T., 2020. Label-free characterization and real-time monitoring of cell uptake of extracellular vesicles. Biosensors and Bioelectronics, 168, 112510. https://doi.org/10.1016/j.bios.2020.112510

Palviainen, M., Laukkanen, K., Tavukcuoglu, Z., Velagapudi, V., Kärkkäinen, O., Hanhineva, K., Auriola, S., Ranki, A. and Siljander, P., 2020. Cancer alters the metabolic fingerprint of extracellular vesicles. Cancers (Basel), 12(11), 3292. https://doi.org/10.3390/cancers12113292

Palviainen, M., Saraswat, M., Varga, Z., Kitka, D., Neuvonen, M., Puhka, M., Joenväärä, S., Renkonen, R., Nieuwland, R., Takatalo, M. and Siljander, P.M.-R., 2020. Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery. PLOS ONE, 15(8), e0236439. https://doi.org/10.1371/journal.pone.0236439

Saari, H., Turunen, T., Lõhmus, A., Turunen, M., Jalasvuori, M., Butcher, S.J., Ylä-Herttuala, S., Viitala, T., Cerullo, V., Siljander, P.R.M. and Yliperttula, M., 2020. Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery. Journal of Extracellular Vesicles, 9(1), 1747206. https://doi.org/10.1080/20013078.2020.1747206

Syrjä, P., Palviainen, M., Jokinen, T., Kyöstilä, K., Lohi, H., Roosje, P., Anderegg, L., Leeb, T., Sukura, A. and Eskelinen, E.L., 2020. Altered basal autophagy affects extracellular vesicle release in cells of Lagotto Romagnolo dogs with a variant ATG4D. Veterinary Pathology, 57(6), pp.926–935. https://doi.org/10.1177/0300985820959243

2019

Arasu, U.T., Deen, A.J., Pasonen-Seppänen, S., Heikkinen, S., Lalowski, M., Kärnä, R., Härkönen, K., Mäkinen, P., Lázaro-Ibáñez, E., Siljander, P.R.-M., Oikari, S., Levonen, A.-L. and Rilla, K., 2019. HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells. Cellular and Molecular Life Sciences. https://doi.org/10.1007/s00018-019-03399-5

Barreiro, K.A., Tulamo, R., Jouppila, A., Albäck, A. and Lassila, R., 2019. Novel locally acting dual antiplatelet and anticoagulant (APAC) targets multiple sites of vascular injury in an experimental porcine model. European Journal of Vascular and Endovascular Surgery, 58(6), pp.903–911. https://doi.org/10.1016/j.ejvs.2019.05.019

Clayton, A., Boilard, E., Buzás, E.I., Cheng, L., Falcón-Pérez, J.M., Gardiner, C., Gustafson, D., Gualerzi, A., Hendrix, A., Hoffman, A., Jones, J., Lässer, C., Lawson, C., Lenassi, M., Nazarenko, I., O’Driscoll, L., Pink, R., Siljander, P.R.-M., Soekmadji, C., Wauben, M., Welsh, J.A., Witwer, K., Zheng, L. and Nieuwland, R., 2019. Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles. Journal of Extracellular Vesicles, 8(1), 1647027. https://doi.org/10.1080/20013078.2019.1647027

Multia, E., Tear, J.C.Y., Palviainen, M., Siljander, P. and Riekkola, M.L., 2019. Fast isolation of highly specific population of platelet-derived extracellular vesicles from blood plasma by affinity monolithic column, immobilized with anti-human CD61 antibody. Analytica Chimica Acta, 1091, pp.160–168. https://doi.org/10.1016/j.aca.2019.08.064

Palviainen, M., Saari, H., Kärkkäinen, O., Pekkinen, J., Auriola, S., Yliperttula, M., Puhka, M., Hanhineva, K. and Siljander, P.R.-M., 2019. Metabolic signature of extracellular vesicles depends on the cell culture conditions. Journal of Extracellular Vesicles, 8(1), 1596669. https://doi.org/10.1080/20013078.2019.1596669

Théry, C., Witwer, K.W., Aikawa, E., Alcaraz, M.J., Anderson, J.D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin-Smith, G.K., ... Siljander, P. et al., 2018. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. Journal of Extracellular Vesicles, 7(1), 1535750. https://doi.org/10.1080/20013078.2018.1535750

Williams, C., Palviainen, M., Reichardt, N.C., Siljander, P.R. and Falcón-Pérez, J.M., 2019. Metabolomics applied to the study of extracellular vesicles. Metabolites, 9(11), 276. https://doi.org/10.3390/metabo9110276

2018

Barok M, Puhka M, Vereb G, Szollosi J, Isola J, Joensuu H. Cancer-derived exosomes from HER2-positive cancer cells carry trastuzumab-emtansine into cancer cells leading to growth inhibition and caspase activation, BMC Cancer. 2018; 18: 504. Published online 2018 May 2. doi: 10.1186/s12885-018-4418-2 PMCID: PMC5930687. (IF 3.288)´

Kornilov R, Puhka M, Mannerström B, Hiidenmaa H, Peltoniemi H, Siljander P, Seppänen-Kaijansinkko R, Kaur S. Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum. J Extracell Vesicles. 2018 Jan 21;7(1):1422674. doi: 10.1080/20013078.2017.1422674. eCollection 2018. (IF 14.976)

2017

Coumans, F.A.W., Brisson, A.R., Buzás, E.I., Dignat-George, F., Drees, E.E.E., El-Andaloussi, S., Emanueli, C., Gasecka, A., Hendrix, A., Hill, A.F., Lacroix, R., Lee, Y., van Leeuwen, T.G., Mackman, N., Mäger, I., Nolan, J.P., van der Pol, E., Pegtel, D.M., Sahoo, S., Siljander, P., Sturk, G., de Wever, O., Nieuwland, R., 2017. Methodological guidelines to study extracellular vesicles. Circulation Research, 120(10), pp.1632–1648. https://doi.org/10.1161/CIRCRESAHA.117.309419

Lázaro-Ibáñez, E., Neuvonen, M., Takatalo, M., Arasu, U.T., Capasso, C., Cerullo, V., Rhim, J.S., Rilla, K., Yliperttula, M. and Siljander, P., 2017. Metastatic state of parent cells influences the uptake and functionality of prostate cancer cell-derived extracellular vesicles. Journal of Extracellular Vesicles, 6(1), 1354645. https://doi.org/10.1080/20013078.2017.1354645

Puhka, M., Takatalo, M., Nordberg, M.-E., Valkonen, S., Nandania, J., Aatonen, M., Yliperttula, M., Laitinen, S., Velagapudi, V., Mirtti, T., Kallioniemi, O., Rannikko, A., Siljander, P.R.-M. and Af Hällström, T.M., 2017. Metabolomic profiling of extracellular vesicles and alternative normalization methods reveal enriched metabolites and strategies to study prostate cancer-related changes. Theranostics, 7(16), pp.3824–3841. https://doi.org/10.7150/thno.19890

Puhka, M., Nordberg, M.E., Valkonen, S., Rannikko, A., Kallioniemi, O., Siljander, P. and Af Hällström, T.M., 2017. KeepEX, a simple dilution protocol for improving extracellular vesicle yield from urine. European Journal of Pharmaceutical Sciences, 98, pp.30–39. https://doi.org/10.1016/j.ejps.2016.11.011

Customer scientific publications

2025

Apostolov, A., Mladenović, D., Tilk, K., Lõhmus, A., Baev, V., Yahubyan, G., Sola-Leyva, A., Bergamelli, M., Görgens, A., Zhao, C., Andaloussi, S.E.L., Kalinina, A., Acharya, G., Lanner, F., Saare, M., Peters, M., Piomboni, P., Luddi, A., Salumets, A. and Aleksejeva, E., 2025. Multi-omics analysis of uterine fluid extracellular vesicles reveals a resemblance with endometrial tissue across the menstrual cycle: biological and translational insights. Human Reproduction Open, 2025(2), hoaf010. https://doi.org/10.1093/hropen/hoaf010

Arvola, O., Stigzelius, V., Ampuja, M. and Kivelä, R., 2025. Neural progenitor cell-derived exosomes in ischemia/reperfusion injury in cardiomyoblasts. BMC Neuroscience, 26(1), p.11. https://doi.org/10.1186/s12868-025-00931-1

Hirvonen, O.P. and Kainulainen, H., 2025. D-glyceric acid enhances the maintenance of glycerophospholipid membranes in 50-60-year-old healthy humans. PREPRINT (Version 1) available at Research Square. doi: 10.21203/rs.3.rs-6128316/v1.

2024

Ilvonen, P., Pusa, R., Härkönen, K., Laitinen, S. and Impola, U., 2024. Distinct targeting and uptake of platelet and red blood cell-derived extracellular vesicles into immune cells. Journal of Extracellular Biology, 3(1), e130. https://doi.org/10.1002/jex2.130

Kankaanpää, S., Nurmi, M., Lampimäki, M., Leskinen, H., Nieminen, A., Samoylenko, A., Vainio, S.J., Mäkinen, S., Ahonen, L., Kangasluoma, J., Petäjä, T. and Viitala, S., 2024. Comparative analysis of the effects of different purification methods on the yield and purity of cow milk extracellular vesicles. Journal of Extracellular Biology, 3(4), e149. https://doi.org/10.1002/jex2.149

Kankaanpää, S., Väisänen, E., Goeminne, G., Soliymani, R., Desmet, S., Samoylenko, A., Vainio, S., Wingsle, G., Boerjan, W., Vanholme, R. and Kärkönen, A., 2024. Extracellular vesicles of Norway spruce contain precursors and enzymes for lignin formation and salicylic acid. Plant Physiology, 196(2), pp.788–809. https://doi.org/10.1093/plphys/kiae287

Lautaoja-Kivipelto, J.H., Karvinen, S., Korhonen, T.M., O'Connell, T.M., Tiirola, M., Hulmi, J.J. and Pekkala, S., 2024. Interaction of the C2C12 myotube contractions and glucose availability on transcriptome and extracellular vesicle microRNAs. American Journal of Physiology-Cell Physiology, 326(2), pp.C348–C361. https://doi.org/10.1152/ajpcell.00401.2023

Liukkonen, M., Heloterä, H., Siintamo, L., Ghimire, B., Mattila, P., Kivinen, N., Kostanek, J., Watala, C., Hytti, M., Hyttinen, J., Koskela, A., Blasiak, J. and Kaarniranta, K., 2024. Oxidative stress and inflammation-related mRNAs are elevated in serum of a Finnish wet AMD cohort. Investigative Ophthalmology & Visual Science, 65(13), p.30. https://doi.org/10.1167/iovs.65.13.30

Pridgeon, C.S., Airavaara, K., Monola, J., Jokela, A., Palmer, D., Yliperttula, M. and Harjumäki, R., 2024. Chronic hypoxia for the adaptation of extracellular vesicle phenotype. Scientific Reports, 14(1), p.25189. https://doi.org/10.1038/s41598-024-73453-1

Punovuori, K., Bertillot, F., Miroshnikova, Y.A., Binner, M.I., Myllymäki, S.M., Follain, G., Kruse, K., Routila, J., Huusko, T., Pellinen, T., Hagström, J., Kedei, N., Ventelä, S., Mäkitie, A., Ivaska, J. and Wickström, S.A., 2024. Multiparameter imaging reveals clinically relevant cancer cell-stroma interaction dynamics in head and neck cancer. Cell, 187(25), pp.7267–7284.e20. https://doi.org/10.1016/j.cell.2024.09.046

2023

Kangas, P., Nyman, T.A., Metsähonkala, L., Burns, C., Tempest, R., Williams, T., Karttunen, J. and Jokinen, T.S., 2023. Towards optimised extracellular vesicle proteomics from cerebrospinal fluid. Scientific Reports, 13(1), p.9564. https://doi.org/10.1038/s41598-023-36706-z

Karvinen, S., Korhonen, T.M., Sievänen, T., Karppinen, J.E., Juppi, H.K., Jakoaho, V., Kujala, U.M., Laukkanen, J.A., Lehti, M. and Laakkonen, E.K., 2023. Extracellular vesicles and high-density lipoproteins: Exercise and oestrogen-responsive small RNA carriers. Journal of Extracellular Vesicles, 12(2), e12308. https://doi.org/10.1002/jev2.12308

Luoto, J.C., Coelho-Rato, L.S., Jungarå, C., Bengs, S.H., Roininen, J., Eriksson, J.E., Sistonen, L. and Henriksson, E., 2023. Cancer cell invasion alters the protein profile of extracellular vesicles. Journal of Extracellular Biology, 2, e124. https://doi.org/10.1002/jex2.124

Saari, H., Pusa, R., Marttila, H., Yliperttula, M. and Laitinen, S., 2023. Development of tandem cation exchange chromatography for high purity extracellular vesicle isolation: The effect of ligand steric availability. Journal of Chromatography A, 1707, 464293. https://doi.org/10.1016/j.chroma.2023.464293

2022

Hiippala, K., Khan, I., Ronkainen, A., Boulund, F., Vähä-Mäkilä, H., Suutarinen, M., Seifert, M., Engstrand, L. and Satokari, R., 2022. Novel strain of Pseudoruminococcus massiliensis possesses traits important in gut adaptation and host-microbe interactions. Gut Microbes, 14(1), p.2013761. doi: 10.1080/19490976.2021.2013761.

Hirvonen, O.P., Lehti, M., Kyröläinen, H. and Kainulainen, H., 2022. Heme Oxygenase-1 and Blood Bilirubin Are Gradually Activated by Oral D-Glyceric Acid. Antioxidants (Basel), 11(12), p.2319. doi: 10.3390/antiox11122319.

Höglund, N., Koho, N., Rossi, H., Karttunen, J., Mustonen, A.M., Nieminen, P., Rilla, K. and Oikari, S., 2022. Isolation of Extracellular Vesicles From the Bronchoalveolar Lavage Fluid of Healthy and Asthmatic Horses. Frontiers in Veterinary Science, 9, p.894189. doi: 10.3389/fvets.2022.894189.

Mustonen, A.M., Lehmonen, N., Oikari, S., Capra, J., Raekallio, M., Mykkänen, A., Paakkonen, T., Rilla, K., Niemelä, T. and Nieminen, P., 2022. Counts of hyaluronic acid-containing extracellular vesicles decrease in naturally occurring equine osteoarthritis. Scientific Reports, 12(1), p.17550. doi: 10.1038/s41598-022-21398-8.

Mustonen, A.M., Paakkonen, T., Matilainen, J., Rilla, K., Käkelä, R., Malinen, M., Takabe, P., Oikari, S., Capra, J., Sihvo, S.P., Ryökäs, P. and Nieminen, P., 2022. Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes. International Journal of Molecular Sciences, 23(10), p.5613. doi: 10.3390/ijms23105613.

Zini, J., Saari, H., Ciana, P., Viitala, T., Lõhmus, A., Saarinen, J. and Yliperttula, M., 2022. Infrared and Raman spectroscopy for purity assessment of extracellular vesicles. European Journal of Pharmaceutical Sciences, 172, p.106135. doi: 10.1016/j.ejps.2022.106135.

2021

Dichlberger, A., Zhou, K., Bäck, N., Nyholm, T., Backman, A., Mattjus, P., Ikonen, E. and Blom, T., 2021. LAPTM4B controls the sphingolipid and ether lipid signature of small extracellular vesicles. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1866(2), p.158855. doi: 10.1016/j.bbalip.2020.158855.

Hirvonen, O.P., Kyröläinen, H., Lehti, M. and Kainulainen, H., 2021. Randomized Trial: D-Glyceric Acid Activates Mitochondrial Metabolism in 50-60-Year-Old Healthy Humans. Frontiers in Aging, 2, p.752636. doi: 10.3389/fragi.2021.752636.

Hirvonen, O.P., Kyröläinen, H. and Kainulainen, H., 2021. Double Blinded Clinical Study on D-Glyceric Acid, a Potential Novel Energy Metabolic Signaling Metabolite. Preprint. doi: 10.21203/rs.3.rs-156795/v1.

Parvanian, S., Zha, H., Su, D., Xi, L., Jiu, Y., Chen, H., Eriksson, J.E. and Cheng, F., 2021. Exosomal Vimentin from Adipocyte Progenitors Protects Fibroblasts against Osmotic Stress and Inhibits Apoptosis to Enhance Wound Healing. International Journal of Molecular Sciences, 22(9), p.4678. doi: 10.3390/ijms22094678.

2020

Hiippala, K., Barreto, G., Burrello, C., Diaz-Basabe, A., Suutarinen, M., Kainulainen, V., Bowers, J.R., Lemmer, D., Engelthaler, D.M., Eklund, K.K., Facciotti, F. and Satokari, R., 2020. Novel Odoribacter splanchnicus Strain and Its Outer Membrane Vesicles Exert Immunoregulatory Effects in vitro. Frontiers in Microbiology, 11, p.575455. doi: 10.3389/fmicb.2020.575455.

Kari, O.K., Tavakoli, S., Parkkila, P., Baan, S., Savolainen, R., Ruoslahti, T., Johansson, N.G., Ndika, J., Alenius, H., Viitala, T., Urtti, A. and Lajunen, T., 2020. Light-Activated Liposomes Coated with Hyaluronic Acid as a Potential Drug Delivery System. Pharmaceutics, 12(8), p.763. doi: 10.3390/pharmaceutics12080763.

Karvinen, S., Sievänen, T., Karppinen, J.E., Hautasaari, P., Bart, G., Samoylenko, A., Vainio, S.J., Ahtiainen, J.P., Laakkonen, E.K. and Kujala, U.M., 2020. MicroRNAs in extracellular vesicles in sweat change in response to endurance exercise. Frontiers in Physiology, 11, p.676. doi: 10.3389/fphys.2020.00676.

Laukkanen, K., Saarinen, M., Mallet, F., Aatonen, M., Hau, A. and Ranki, A., 2020. Cutaneous T-Cell Lymphoma (CTCL) Cell Line-Derived Extracellular Vesicles Contain HERV-W-Encoded Fusogenic Syncytin-1. Journal of Investigative Dermatology, 140(7), pp.1466-1469.e4. doi: 10.1016/j.jid.2019.11.021.

Schneegans, S., Lück, L., Besler, K., Bluhm, L., Stadler, J.-C., Staub, J., Greinert, R., Volkmer, B., Kubista, M., Gebhardt, C., Sartori, A., Irwin, D., Serkkola, E., Af Hällström, T., Lianidou, E., Sprenger-Haussels, M., Hussong, M., Mohr, P., Schneider, S.W., Shaffer, J., Pantel, K. and Wikman, H., 2020. Pre-analytical Factors Affecting the Establishment of a Single Tube Assay for Multiparameter Liquid Biopsy Detection in Melanoma Patients. Molecular Oncology, 14(5), pp.1001-1015. doi: 10.1002/1878-0261.12669.

2019

Kloten, V., Neumann, M.H.D., Di Pasquale, F., Sprenger-Haussels, M., Shaffer, J.M., Schlumpberger, M., Herdean, A., Betsou, F., Ammerlaan, W., Af Hällström, T., Serkkola, E., Forsman, T., Lianidou, E., Sjöback, R., Kubista, M., Bender, S., Lampignano, R., Krahn, T. and Schlange, T., 2019. Multicenter Evaluation of Circulating Plasma MicroRNA Extraction Technologies for the Development of Clinically Feasible Reverse Transcription Quantitative PCR and Next-Generation Sequencing Analytical Work Flows. Clinical Chemistry, 65(9), pp.1132-1140. doi: 10.1373/clinchem.2019.303271.

Mannerström, B., Kornilov, R., Abu-Shahba, A.G., Chowdhury, I.M., Sinha, S., Seppänen-Kaijansinkko, R. and Kaur, S., 2019. Epigenetic alterations in mesenchymal stem cells by osteosarcoma-derived extracellular vesicles. Epigenetics, 14(4), pp.352-364. doi: 10.1080/15592294.2019.1585177.

2018

Hoornstra, D., Vesterlin, J., Pärnänen, P., Al-Samadi, A., Zlotogorski-Hurvitz, A., Vered, M. and Salo, T., 2018. Fermented Lingonberry Juice Inhibits Oral Tongue Squamous Cell Carcinoma Invasion In Vitro Similarly to Curcumin. In Vivo, 32(5), pp.1089-1095. doi: 10.21873/invivo.11350.

Gebraad, A., Kornilov, R., Kaur, S., Miettinen, S., Haimi, S., Peltoniemi, H., Mannerström, B. and Seppänen-Kaijansinkko, R., 2018. Monocyte-derived extracellular vesicles stimulate cytokine secretion and gene expression of matrix metalloproteinases by mesenchymal stem/stromal cells. FEBS Journal, 285(12), pp.2337-2359. doi: 10.1111/febs.14485.

Kaur, S., Abu-Shahba, A.G., Paananen, R.O., Hongisto, H., Hiidenmaa, H., Skottman, H., Seppänen-Kaijansinkko, R. and Mannerström, B., 2018. Small non-coding RNA landscape of extracellular vesicles from human stem cells. Scientific Reports, 8(1). doi: 10.1038/s41598-018-33899-6.

2017

Al-Samadi, A., Awad, S.A., Tuomainen, K., Zhao, Y., Salem, A., Parikka, M. and Salo, T., 2017. Crosstalk between tongue carcinoma cells, extracellular vesicles, and immune cells in in vitro and in vivo models. Oncotarget, 8(36), pp.60123-60134. doi: 10.18632/oncotarget.17768.