Instrument | prime time (9-17) | Other time | Night (22-08) |
---|---|---|---|
Leica TCS SP8 STED | 22€/h | 17€/h | 13€/h |
Leica TCS SP8 Upright | 20€/h | 16€/h | 12€/h |
Leica Stellaris 8 FALCON | 22€/h | 17€/h | 13€/h |
Leica DM 6000B | 5€/h | 3€/h | - |
Zeiss LightSheet Z.1 | 18€/h | 14€/h | 10€/h |
Light sheet Image processing | 2€/h | 2€/h | 1€/h |
3I Marianas with lasers | 18€/h | 14€/h | 10€/h |
3I Marianas without lasers | 9€/h | 7€/h | 5€/h |
3i Marianas Aurox Clarity | 9€/h | 7€/h | 5€/h |
Celldiscoverer7 with LSM900 | 18€/h | 14€/h | - |
Celldiscoverer 7 widefield | 9€/h | 7€/h | - |
Olympus FV4000 | 22€/h | 17€/h | 13€/h |
ONI Nanoimager S | 18€/h | 14€/h | 10€/h |
Aurox Clarity | 9€/h | 7€/h | 5€/h |
GE Deltavision Ultra | 9€/h | 7€/h | 5€/h |
MD Image Express Micro | 9€/h | 7€/h | 5€/h |
Cell-IQ | 2€/h | 2€/h | - |
3D workstation | 5€/h | 3€/h | - |
LS workstation | 5€/h | 3€/h | - |
HELMI 3D VM | 5€/h | 3€/h | - |
HCA workstation | 3€/h | 1€/h | - |
2D workstation | 3€/h | 1€/h | - |
Operator | 80€/h | - | - |
Prolong Diamond antifade reagent | 75€/2mL |
Slowfade Diamond antifade reagent | 55€/2mL |
Mattek dish (uncoated, #1.5) | 25€/sleeve |
Mattek dish (uncoated, #0) | 20€/sleeve |
Mattek dish (collagen coated) | 25€/sleeve |
Mattek dish (polylysine coated) | 25€/sleeve |
Mattek dish (gridded, uncoated) | 40€/sleeve |
Mattek dish (high precision, uncoated) | 25€/sleeve |
Greiner 35mm dish, glass bottom | 40€/sleeve |
Greiner 35mm dish, glass bottom, 4 compartments | 80€/sleeve |
Greiner Cellview 96 well, glass bottom | 50€/plate |
Greiner CellView 10 well slide, Advanced TC, coverglass bottom | 19€/slide |
PerkinElmer PhenoPlate 96 Ultra TC, 190um bottom, cyclic olefin | 10€/plate |
Ibidi µ-plate 24 well, Ibidi treat, 180um bottom | 22€/plate |
VisionPlate 24 well, 190um polystyrene TC | 10€/plate |
SPL culture plate 6 well, 13mm #1 glass | 13€/plate |
High precision cover slips 18x18mm (200 pcs/box) | 9€/box |
High precision cover slips round 18mm (100 pcs/box) | 28€/box |
High precision cover slips round 13mm (100 pcs/box) | 17€/box |
High precision cover slips round 10mm (100 pcs/box) | 15€/box |
Gridded and high precision Mattek dishes have 5 dishes per sleeve, the others have 10 dishes per sleeve.
Instrument | prime time (9-17) | Other time | Night (22-08) |
---|---|---|---|
Leica TCS SP8 STED | 132€/h | 102€/h | 78€/h |
Leica TCS SP8 Upright | 120€/h | 96€/h | 72€/h |
Leica Stellaris 8 FALCON | 132€/h | 102€/h | 78€/h |
Leica DM 6000B | 30€/h | 18€/h | - |
Zeiss LightSheet Z.1 | 108€/h | 84€/h | 60€/h |
LightSheet image processing | 12€/h | 12€/h | 6€/h |
3I Marianas with lasers | 108€/h | 84€/h | 60€/h |
3I Marianas without lasers | 54€/h | 42€/h | 30€/h |
3i Marianas Aurox Clarity | 54€/h | 42€/h | 30€/h |
Celldiscoverer 7 with LSM900 | 108€/h | 84€/h | |
Celldiscoverer 7 widefield | 54€/h | 42€/h | |
Olympus FV4000 | 132€/h | 102€/h | 78€/h |
ONI Nanoimager S | 108€/h | 84€/h | 60€/h |
Aurox Clarity | 54€/h | 42€/h | 30€/h |
GE Deltavision Ultra | 54€/h | 42€/h | 30€/h |
MD ImageExpress Micro | 54€/h | 42€/h | 30€/h |
Cell-IQ | 12€/h | 12€/h | - |
3D workstation | 30€/h | 18€/h | - |
LS workstation | 30€/h | 18€/h | - |
HELMI 3D VM | 30€/h | 18€/h | - |
HCA workstation | 18€/h | 6€/h | - |
2D workstation | 18€/h | 6€/h | - |
Operator | 80€/h | - | - |
Prolong Diamond antifade reagent | 150€/2mL |
Slowfade Diamond antifade reagent | 110€/2mL |
Mattek dish (uncoated, #1.5) | 50€/sleeve |
Mattek dish (uncoated, #0) | 40€/sleeve |
Mattek dish (collagen coated) | 50€/sleeve |
Mattek dish (polylysine coated) | 50€/sleeve |
Mattek dish (gridded, uncoated) | 80€/sleeve |
Mattek dish (high precision, uncoated) | 50€/sleeve |
Greiner 35mm dish, glass bottom | 80€/sleeve |
Greiner 35mm dish, glass bottom, 4 compartments | 160€/sleeve |
Greiner Cellview 96 well, glass bottom | 100€/plate |
Greiner CellView 10 well slide, advanced TC, coverglass bottom | 38€/slide |
PerkinElmer PhenoPlate 96 Ultra TC, 190um cyclic olefin bottom | 20€/plate |
Ibidi µ-plate 24 well, Ibidi treat, 180um bottom | 44€/plate |
VisionPlate 24 well, 190um polystyrene TC | 20€/plate |
SPL culture plate 6 well, 13mm #1 glass bottom | 26€/plate |
High precision cover slips 18x18mm (200 pcs/box) | 18€/box |
High precision cover slips round 18mm (100 pcs/box) | 56€/box |
High precision cover slips round 13mm (100 pcs/box) | 34€/box |
High precision cover slips round 10mm (100 pcs/box) | 30€/box |
Gridded and high precision Mattek dishes have 5 dishes per sleeve, the others have 10 dishes per sleeve.
All users are welcome to use LMU instruments. To gain access you must first register your imaging project in the Open IRIS reservation system and arrange a short meeting with us (see Open IRIS instructions on our Wiki). A training session will be arranged based on the meeting, and after the training you will be granted reservation rights for the instrument. You can contact us by e-mail (lmu-staff at helsinki dot fi) or by phone.
The above-mentioned meeting is compulsory. You are required to prepare a short presentation (~5 min) about your project so that we can discuss specifics and possible problems. This allows us to choose the right instrument and tailor the training according to your needs. If you do not have previous experience with confocals the training is typically a 3-hour session, depending on your samples. User training is charged according to the duration of the training session.
An active imaging project registered in Open IRIS is required for continued reservation rights. The default duration of an imaging project is one year. After one year the project must be updated, though if you know you are finishing the project soon, we can extended the end date a few months. If you start a new project, you should register the new project in Open IRIS.
Users from outside of the Institute of Biotechnology also need to fill in the "LMU key activation form" to gain access to LMU facilities. A link to the form is on LMU home page.
If you are doing live imaging with GMO samples, the microscope rooms have to be included in your GMO work application. Human pathogens are not allowed on LMU premises. You are not allowed to touch the microscope controls, door handles, the keyboard or the screen while wearing gloves. If you need to use gloves to put in your sample, you must remove your gloves before touching anything else.
Visualization of live BSL2 samples, such as virus-infected cells, is, at the moment, possible only with the 3I Marianas system and always requires prior approval and discussions with the scientific director of LMU, Maria Vartiainen (maria.vartiainen@helsinki.fi).
Samples must be prepared in your own lab using the appropriate safety procedures.
Samples should be prepared on SBS format multiwell plates (instead of e.g. Mattek-dishes) to minimize the chance of spilling the sample inside the microscope. Plates should be sealed with parafilm and the outside of the plate wiped with an appropriate disinfectant.
Samples must be carried to LMU in a closed secondary container with absorbent materials in the bottom of the container.
After imaging, remove the sample from the microscope and wipe the microscope stage with tissue soaked in 70% ethanol (or another appropriate disinfectant). Any spills must be cleaned immediately and thoroughly with an appropriate disinfectant. All spills must be reported immediately to the LMU staff.
All samples and other material including used tissues must be taken back to the host lab for disposal, transported in a secondary container as above.
Reservations for LMU microscopes and workstations are made in Open IRIS (see instructions on our Wiki). LMU instrument computers and workstations with Windows 10 or Windows 11 operating systems are running Open IRIS Agent software that requires you to log in to Open IRIS. The booking has to be for the same user who is using the instrument. The Agent records the time when you log in and log out, modifying the end time of the session automatically. You can also manually extend the session as long as there are no conflicts to other reservations. You may cancel a booking only when a start time of the booking is more than 2 hours in future. Similarly, start point of reservation cannot be moved into the future, nor end time towards the present if the reservation would start within 2 hours of the current time. Reservations can only be modified by the person who has made them or by an administrator. If you want to shorten your reservation but can't, contact LMU. All extra usage that has not been properly reported to us within two working days will be charged 100% extra. No-shows will be billed as normal. We ask you to make reservations so that prime time is used efficiently - i.e. avoid leaving one hour gaps unnecessarily.
Minimum reservation time is 1h except for workstations, Leica DM6000 and MD Nano where minimum is 30 minutes. You are not allowed to use equipment without reservation. Pricing and usage limits are based on the following: Prime Time (workdays 9-17), Night Time (every day 22-08) and Other Time (not Prime or Night Time). Night Time pricing is not available on all systems.
Reservations have time limits as shown below (longer reservations by separate agreement):
However, these limits are not applied if you are doing the booking for the current day.
Reservations can be made up to 21 days in advance. If you need to reserve any of our equipment further in advance, please contact us.
When making a reservation you must book the lasers you are going to use (Add-On in OpenIRIS) . This is important information in order to let the previous user know which lasers can be shut down to extend their life time.
Depending on the system, +29C and +37C heating options are available. Reservations with heating are indicated with color in Open IRIS and Open IRIS automatically enforces warm-up and cool-down periods between bookings in different temperatures (shown by gray in Open IRIS). If you still want to use the microscope during the warm up or cooling down, ask LMU staff to make a reservation for you, though we strongly advice against this. Notably LightSheet microscope does not take extra time to change temperature.
In general cancellations must be done 2h before the reservation starts. We can cancel a reservation after this if there is a pressing reason.
Before finishing your session, always check in the reservation system what the next user will do, and conclude your session accordingly.
Billing is based on a time the user has been logged in at computer at a resource. Price list for the resources. Accumulated bills will be sent out to the groups approximately four times per year. Time spent solving hardware problems is not charged if the time exceeds 30 minutes.
LMU staff provides basic training and troubleshooting during prime time at no extra cost. More advanced and customized projects can be done as scientific collaboration. In exceptional cases, LMU staff can also image your samples for you as fee for service. On scientific collaboration we charge the used equipment time and on fee for service the equipment time + operator cost.
LMU has two email lists:
Messages to the email lists are also put into the Yammer group Light Microscopy at Viikki. List membership is mandatory for the users.
You may store microscopy data on lmu_active disk for two years. After that the data is moved into another space for a half year. Data from users without active project is similarly removed to separate space for a half year. Older data will be moved semi-automatically to L:\lmu-archive and this transfer is based on modification time. Please note that this may break your datasets. Importantly, the user is responsible for real archival of the data.
LMU retains the right to manage user data on LMU network storage and LMU administrated computers (including HY-data and LocalData folders) in order to free diskspace as necessary by moving data therein to external non-secure media.
LMU should be mentioned in the acknowledgements in research publications containing imaging data acquired in or with support of LMU, or when LMU staff contributed significantly to the image analysis. Here are examples of a sentences that can be used:
We kindly ask to send a notification about your LMU-supported publication to the head of the imaging facility: Kimmo.tanhuanpaa@helsinki.fi