Mitochondrial function relies on the complex mitochondrial shape, membrane ultrastructure and dynamics, which abnormalities lead to organelle dysfunction and eventually cell death. Our work focus on the molecular mechanism of mitochondrial membrane architectural organization and factors involved in the regulation of mitochondrial shape and dynamics. We combine a wide array of cell biology, advanced proteomics, biochemistry/biophysics, and structural biology methods to identify and characterize novel proteins critical for mitochondrial morphogenesis and dynamics, to address the underlying molecular mechanisms and their functional link to mitochondrial diseases. In addition, our lab is interested in elucidating how proteins sculpt divergent membrane structures in cells and how signal lipids regulate protein functions, most importantly how membrane morphogenesis is linked to central cellular processes.