Mitochondrial function depends on the intricate shape, membrane ultrastructure, and dynamics of the organelle. Abnormalities in these aspects can lead to organelle dysfunction and ultimately result in cell death. Our research focuses on the molecular mechanisms governing mitochondrial membrane architecture and the factors that regulate mitochondrial shape and dynamics. We employ a comprehensive array of cell biology techniques, advanced proteomics, biochemistry/biophysics, and structural biology methods to identify and characterize novel proteins essential for mitochondrial morphogenesis and dynamics. Our goal is to elucidate the molecular mechanisms underlying these processes and their functional links to mitochondrial diseases. Additionally, our lab investigates how proteins shape diverse membrane structures within cells and how signaling lipids regulate protein functions, with a particular emphasis on the connection between membrane morphogenesis and central cellular processes.