DPBM-210-2023
DPBM-210 Light mi­cro­scopy From ba­sics to ad­vanced
Light microscopy course organized by BIU

On-site lectures  09. - 10.2.2023 + demos 13. - 17.2.2023

 

Registration: https://elomake.helsinki.fi/lomakkeet/121818/lomake.html AND https://studies.helsinki.fi/courses/cur/hy-opt-cur-2223-c1a91580-6159-4…

If you want to earn credits, please register in elomake AND Sisu

 

Learning Outcomes:

After completing this course the students should be able to

  • explain the basic principles of light microscopy and microscopic image acquisition
  • understand the most important steps of sample preparation for microscopy
  • compare and contrast transmitted light and fluorescence methods
  • compare and contrast widefield and optical sectioning microscopy methods
  • know some basic and advanced research applications for the microscopy methods covered
  • evaluate the suitability of covered microscopy methods for different research applications

 

Credits:

2 ECTS for completing the course (2 lecture days, 3 demos, home assignment)

 

Course Contents:

 

Lectures (everybody welcome)

9.2.2023

Lecture Hall 1

9:30-11:00 Introduction to light microscopy and image acquisition

11:15-12:00 Confocal microscopy

12:00-12:30 Scaling up your imaging (speed, resolution and throughput)

Seminar Room 1-2

14:00-14:45 Lightsheet microscopy and sample clearing

15:00-15:45 Multiphoton, intravital, and label-free imaging

15.2.2023

Lecture Hall 2

16:00-16:45 Fluorescence Lifetime imaging and FRET

 

For course participants:

10.2.2023

Optics interactive lecture in smaller groups (2h each)

 

13. - 17.2.2023

Demos  (3x  2-3h)

Students should choose one of the options from each demo from the followings:

Demo 1:

  •     Widefield brightfield and fluorescence (basic)
  •     Widefield imaging of pre-set points, mosaic (tile) images, autofocus and focus maps
  •     3D widefield imaging with “optical clearing”
  •     Automated widefield imaging of well plates/slides with the Pico

Demo 2:

  •     Scanning confocal microscopy (basic)
  •     Improving confocal resolution by Lightning
  •     Improving confocal resolution by Airyscan

Demo 3:

  •     Widefield imaging of pre-set points, mosaic (tile) images, autofocus and focus maps
  •     3D widefield imaging with “optical clearing”
  •     Automated widefield imaging of well plates/slides with the Pico
  •     Improving confocal resolution by Lightning
  •     Improving confocal resolution by Airyscan
  •     Digital light sheet for smaller samples (for example organoids, zebrafish, drosophila embryos)
  •     Light sheet for solvent-cleared bigger samples (for example mouse organs like brain, kidney, lung)
  •     FLIM/FRET
  •     Intravital microscopy of skin vasculature