FIMM Genomics Transcriptomics sample delivery

RNA samples

• Samples should be delivered in a skirted 96-well plate in column wise orientation (A1, B1, C1...) marked with a date and the project name (sample batch ID in iLab) 
  • There should be no empty wells or columns between samples. Full reaction cost will be charged for empty wells.
  • Recommended plate: skirted Eppendorf™ twin.tec™ 96 Well LoBind PCR Plate (Catalog no. 0030129512)
  • Plates must be sealed carefully with a PCR plate seal 
  • Sample delivery on dry ice
  • Sample information including sample ID, well, concentration in ng/ul, volume, source material, and possible batch information through iLab
  • Reference genome information
• We have no capacity for long time sample storage so delivered RNA samples are for one-time use only.

Illumina Stranded Total RNA Prep with Ribo-Zero Plus

• DNase I -treated total RNA samples in at least 20 µl of nuclease free-water
  • Minimum concentration of 5 ng/µl of good quality (RIN ≥ 8, Agilent Bioanalyzer) total RNA
  • DNA contamination of the samples should be less than 10%
  • Library prep input amount should be 10 - 1000 ng 

Illumina Stranded mRNA Prep 

• DNase I -treated total RNA samples in at least 30 µl of nuclease free-water 
  • Minimum concentration of 5 ng/µl of good quality (RIN ≥ 8, Agilent Bioanalyzer) total RNA
  • DNA contamination of the samples should be less than 10%
  • Library prep input amount should be 50 - 1000 ng (recommended input 500 ng)
  • The kit does not accept purified mRNA as input

Quantabio sparQ RNA-Seq HMR kit

• Total RNA samples in 20 µl nuclease-free water
  • Minimum concentration of 0.2 ng/µl of good quality (RIN ≥ 7, Agilent Bioanalyzer) total RNA. For degraded and/or FFPE RNA 2 ng/µl of total RNA.
  • DNA contamination of the samples should be less than 10%
  • Library prep input amount should be 1 - 1000 ng for high-quality total RNA and 10-1000 ng for degraded and/or FFPE RNA 

Watchmaker RNA Library Prep Kit with Polaris™ Depletion

• Total RNA samples in 30 µl nuclease-free water
  • Minimum concentration of 0.1 ng/µl of good quality (RIN ≥ 7, Agilent Bioanalyzer) total RNA. For degraded and/or FFPE RNA 1 ng/µl of total RNA.
  • DNA contamination of the samples should be less than 10%
  • Library prep input amount 1 - 1000 ng for high-quality total RNA or 10 – 1000 ng for partially degraded and/or FFPE RNA

Lexogen Small RNA-seq library prep

• DNase I -treated total RNA samples with miRNA fraction in 20 µl of nuclease-free water
  • Minimum concentration of 5 ng/µl of good quality (RIN ≥ 8, Agilent Bioanalyzer) total RNA
  • DNA contamination of the samples should be less than 10%
  • Library prep input amount should be 50 pg – 1000 ng (recommended input ≥100ng)

SMART-Seq mRNA 

• Total RNA samples in 20 µl nuclease-free water
  • Minimum concentration of 0.1 ng/µl of good quality (RIN ≥ 8, Agilent Bioanalyzer) total RNA
  • DNA contamination of the samples should be less than 10%
  • Library prep input amount should be 10 pg – 100 ng 

PacBio Kinnex full-length RNA kit

• Total RNA samples in 20 µl nuclease-free water
  • Minimum concentration of 50 ng/µl of good quality (RIN ≥ 7, Agilent Bioanalyzer) total RNA. 
  • Library prep input amount 300 ng

Oxford Nanopore cDNA-PCR Sequencing V14 library prep

• Total RNA samples in 20 µl nuclease-free water
  • Minimum concentration of 50 ng/µl of good quality (RIN ≥ 9, Agilent Bioanalyzer) total RNA. 
  • Library prep input amount 500 ng

When your iLab service request has been approved, you can contact core staff and schedule sample delivery.

If you are sending samples by mail or courier service, use the following address:

FIMM Technology Centre

Genomics NGS Sequencing

Biomedicum 2U, 2nd floor, F-wing

Tukholmankatu 8

00290 Helsinki

RNA samples should always be delivered on dry ice.