Monolith NT.115
Microscale Thermophoresis (MST) detects changes in the hydration shell of molecules and measures biomolecular interactions under close to native conditions.

Technology uses infrared-lasers to create precise microscale temperature gradient within glass capillaries and molecules move along these temperature gradients. When molecules bind or dissociate, the change in their primary, secondary, tertiary and/or quaternary structure changes the hydration shell of the biomolecule and affects the thermophoretic movement. This movement is used to determine binding from single metal ions to the formation of protein complexes with a dynamic range from mM to pM affinities.

Possible MST applications are:

  • Protein-Protein/Peptide
  • Protein-Nucleic Acid
  • Protein-Compound
  • Protein-Vesicle
  • Nucleic Acid-Nucleic Acid
  • Ribosome-Protein, Ribosome-Small molecule
  • Protein modifications as phosphorylation
Technical specifications

Our instrument is equipped with one Nano detector with two fluorescent channels, Nano-Blue/Red.

  • affinity range 1 nM to mM
  • volume per measurement < 4 µl
  • concentration of fluorescent molecule 10-9 - 10-3 M
  • samples per run 16
  • molecular weight range (Da) 101 - 107
  • temperature control 22 - 45°C

Fluorescense exitations and emissions, pdf

Manuals

Monolith NT.115 uses MO Control software for data aquisition and MO Affinity Analysis software for data analysis. Manuals for labelling proteins or antibodies are included in the labeling kits, instrument and software specific manuals are listed below.

Monolith NT.115 Instrument, pdf

Getting started, pdf

MO.Control, pdf

MO.Affinity Analysis, pdf

Consumables

Customers buy all needed consumables (tips, labelling kits, capillaries etc.). We keep a small stock of standard and special treated capillaries which customers are able to test. Ordering of consumables is done through Nanotemper webpages.