Technology uses infrared-lasers to create precise microscale temperature gradient within glass capillaries and molecules move along these temperature gradients. When molecules bind or dissociate, the change in their primary, secondary, tertiary and/or quaternary structure changes the hydration shell of the biomolecule and affects the thermophoretic movement. This movement is used to determine binding from single metal ions to the formation of protein complexes with a dynamic range from mM to pM affinities.
Possible MST applications are:
Our instrument is equipped with one Nano detector with two fluorescent channels, Nano-Blue/Red.
Monolith NT.115 uses MO Control software for data aquisition and MO Affinity Analysis software for data analysis. Manuals for labelling proteins or antibodies are included in the labeling kits, instrument and software specific manuals are listed below.
Below some application notes from Nanotemper to help you with experiment design
Customers buy all needed consumables (tips, labelling kits, capillaries etc.). We keep a small stock of standard and special treated capillaries which customers are able to test. Ordering of consumables is done through Nanotemper webpages.