Zeiss PALM MicroBeam laser microdissection instrument is located at the EE building (Agnes Sjöbergin katu 2), 3rd floor, lab 3121.
It can be used to dissect and collect samples from histological sections and cell cultures, down to one-cell level and even below. A typical downstream application is RNA or DNA isolation.
The instrument is built on the Axio Observer.Z1 body. It has a basic epifluorescence equipment, two digital cameras and a hood. For complete specifications, please see the list below.
How to get started
The instrument is available to all users, after a short initial training. An hourly fee of 30€ (University of Helsinki users) / 60€ (other users, VAT(alv) 0%) is applied to cover for the running costs. Basic disposables such as membrane slides and capture tubes can be purchased from the laboratory on cost recovery basis; please note that we are not routinely stocking all disposables so contact us in advance).
To apply for access, please contact the administrator by email to lcm-admin(at)helsinki(dot)fi, specifying the following details:
- Your name, email, phone number and work address
- Your 8-letter username in the university IT system
- Billing address and contact details for the person who will pay the instrument usage fees
- Your unit code (tulosyksikkö)
- Invoice checker's name (laskun asiatarkastajan nimi)
- Project code / acronym for cost assignment (maksuprojektin koodi/akronyymi)
- What kind of samples you are planning to dissect
Manuals and protocols are available in the Zeiss PALM MicroBeam Moodle area:
Booking the instrument
The instrument can be booked using a Google Calendar account to which you are granted access upon registering as a user. Please include a short description of what kind of samples you are processing.
Existing bookings can be publicly viewed here.
Users' mailing list
To receive notifications concerning MicroBeam users, subscribe to our users' mailing list by sending an email to firstname.lastname@example.org, with no subject, and "subscribe lcm-viikki (at) Helsinki(dot)fi" as the message body.
The MicroBeam team
Mikael Niku and Krista Weber (Division of Veterinary Biosciences) are currently responsible for running the instrument.