ViPS Invited Seminar November 2020

Miguel Aranda Regulés, Spanish National Research Council, Spain

Date: 18th November 2020

Time: 13:00

Title: Loss-of-susceptibility to pepino mosaic virus in tomato

Location: Remotely via Zoom

Host: Kristiina Mäkinen

Abstract: Pepino mosaic virus (PepMV) causes an important disease in tomato crops worldwide. Sources of partial resistance to PepMV have been identified in the natural diversity of Solanum spp., but they seem to be controlled by complex genetics and/or are specific to the viral strain. We thus have addressed alternative strategies for the development of tomato varieties resistant to PepMV, including the screening of an EMS mutagenized collection of tomato lines, and the targeting of genes encoding potential susceptibility factors. With the first strategy we identified a mutant line showing loss-of-susceptibility to PepMV. A mapping-by-sequencing approach pointed to a genetic interval which encompasses four candidate genes carrying single nucleotide polymorphisms. For the second strategy, we focussed on genes encoding tomato proteins interacting with PepMV proteins in a yeast-two-hybrid screening. Generation of mutants using the CRISPR/Cas9 technology enabled the identification of GSTU38 as a proviral factor for this virus.

Research in Miguel Aranda´s group is mainly in genetic resistant to agronomically important plant viruses.

Read more about Miguel's work

Related publications:

P. GÓMEZ, R.N. SEMPERE, M.A. ARANDA (2012). Pepino Mosaic Virus and Tomato Torrado Virus: Two Emerging Viruses Affecting Tomato Crops in the Mediterranean Basin. Advances In Virus Research, 84:505-532.

X. AGIRREZABALA, E. MÉNDEZ-LÓPEZ, G. LASSO, M.A. SÁNCHEZ-PINA, M.A. ARANDA, M. VALLE (2015). The near-atomic cryoEM structure of a flexible filamentous plant virus shows homology of its coat protein with nucleoproteins of animal viruses. eLIFE, 4:e11795.

J. AGUERO, C. GOMEZ-AIX, R.N. SEMPERE, J. GARCIA-VILLALBA, J. GARCIA-NUNEZ, Y. HERNANDO, M.A. ARANDA (2018). Stable and Broad Spectrum Cross-Protection Against Pepino Mosaic Virus Attained by Mixed Infection. Frontiers in Microbiology 9, 1810.

H.T. LE DUC, E. MENDEZ-LOPEZ, C. WANG, U. COMMANDEUR, M.A. ARANDA, N.F. STEINMETZ (2019). Biodistribution of Filamentous Plant Virus Nanoparticles: Pepino Mosaic Virus versus Potato Virus X. Biomacromolecules 20:469-477

F. RUIZ-RAMÓN, R.N. SEMPERE, E. MÉNDEZ-LÓPEZ, M.A. SÁNCHEZ-PINA, M.A. ARANDA (2019). Second generation of pepino mosaic virus vectors: Improved stability in tomato and a wide range of reporter genes. Plant Methods, 15:58.