Software

We develop software tools for image processing of cryo-EM data. All software is open source and made available via our GitHub repository. 

Cryogenic electron microscopy (cryoEM) can yield near-atomic resolution structures of highly ordered macromolecular complexes. Often however some subunits bind in a flexible manner, have different symmetry from the rest of the complex, or are present in sub-stoichiometric amounts, limiting the attainable resolution. We have developed a general method for the localized three-dimensional reconstruction of such subunits earlier (Ilca et al. 2015 Nature Commun). In this project we implement Localized Reconstruction as a plugin (Locarec) for Scipion.