High throughput Arabidopsis Analysis

We have taken a genomic approach to identify flower related Ubiquitin Proteasome System components from the RING type ubiquitin E3 ligase protein family.

The Arabidopsis RING E3 ligases were retrieved and verified in the Arabidopsis genome v11 (Pavicic et al., 2017). They were categorized into flower organs or developmental stages based on their differential gene expression at the Genevestigator database (ref?). Representative mutants were HTP screened for growth and morphological phenotypes. Simultaneously, a workflow was developed for high throughput phenotypic screening of growth, morphology and flowering of nearly a thousand Arabidopsis plants in one experimental round (Pavicic et al., 2017).